Experiment: Formate with Nitrofurantoin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04970 and MMP_RS04975 are separated by 85 nucleotides MMP_RS04975 and MMP_RS04980 are separated by 340 nucleotides
MMP_RS04970: MMP_RS04970 - F420-dependent thioredoxin reductase, at 947,275 to 948,177
_RS04970
MMP_RS04975: MMP_RS04975 - hypothetical protein, at 948,263 to 949,270
_RS04975
MMP_RS04980: MMP_RS04980 - DUF2193 domain-containing protein, at 949,611 to 951,110
_RS04980
Position (kb) 948
949
950 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 947.273 kb on - strand at 947.293 kb on - strand at 947.392 kb on + strand, within MMP_RS04970 at 947.434 kb on + strand, within MMP_RS04970 at 947.488 kb on - strand, within MMP_RS04970 at 947.635 kb on - strand, within MMP_RS04970 at 947.653 kb on - strand, within MMP_RS04970 at 947.674 kb on - strand, within MMP_RS04970 at 947.675 kb on + strand, within MMP_RS04970 at 947.675 kb on + strand, within MMP_RS04970 at 947.694 kb on - strand, within MMP_RS04970 at 947.695 kb on + strand, within MMP_RS04970 at 947.706 kb on - strand, within MMP_RS04970 at 947.706 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.708 kb on - strand, within MMP_RS04970 at 947.786 kb on + strand, within MMP_RS04970 at 947.828 kb on + strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.847 kb on - strand, within MMP_RS04970 at 947.863 kb on - strand, within MMP_RS04970 at 947.863 kb on - strand, within MMP_RS04970 at 947.864 kb on + strand, within MMP_RS04970 at 947.866 kb on - strand, within MMP_RS04970 at 947.883 kb on + strand, within MMP_RS04970 at 947.925 kb on - strand, within MMP_RS04970 at 947.930 kb on + strand, within MMP_RS04970 at 948.172 kb on - strand at 948.202 kb on - strand at 948.203 kb on + strand at 948.220 kb on + strand at 948.221 kb on - strand at 948.223 kb on - strand at 948.223 kb on - strand at 948.223 kb on - strand at 948.228 kb on - strand at 948.228 kb on - strand at 948.229 kb on + strand at 948.272 kb on + strand at 948.305 kb on + strand at 948.461 kb on - strand, within MMP_RS04975 at 948.602 kb on - strand, within MMP_RS04975 at 948.602 kb on - strand, within MMP_RS04975 at 948.604 kb on - strand, within MMP_RS04975 at 948.752 kb on - strand, within MMP_RS04975 at 948.791 kb on - strand, within MMP_RS04975 at 948.791 kb on - strand, within MMP_RS04975 at 948.792 kb on + strand, within MMP_RS04975 at 948.798 kb on - strand, within MMP_RS04975 at 948.798 kb on - strand, within MMP_RS04975 at 948.798 kb on - strand, within MMP_RS04975 at 948.799 kb on + strand, within MMP_RS04975 at 948.799 kb on + strand, within MMP_RS04975 at 948.799 kb on + strand, within MMP_RS04975 at 948.803 kb on - strand, within MMP_RS04975 at 948.803 kb on - strand, within MMP_RS04975 at 948.803 kb on - strand, within MMP_RS04975 at 948.803 kb on - strand, within MMP_RS04975 at 948.804 kb on + strand, within MMP_RS04975 at 948.806 kb on + strand, within MMP_RS04975 at 948.806 kb on + strand, within MMP_RS04975 at 948.806 kb on + strand, within MMP_RS04975 at 948.826 kb on - strand, within MMP_RS04975 at 948.831 kb on - strand, within MMP_RS04975 at 948.831 kb on - strand, within MMP_RS04975 at 948.832 kb on + strand, within MMP_RS04975 at 948.840 kb on + strand, within MMP_RS04975 at 948.904 kb on + strand, within MMP_RS04975 at 948.904 kb on + strand, within MMP_RS04975 at 948.904 kb on + strand, within MMP_RS04975 at 948.928 kb on + strand, within MMP_RS04975 at 948.938 kb on - strand, within MMP_RS04975 at 948.939 kb on + strand, within MMP_RS04975 at 948.943 kb on - strand, within MMP_RS04975 at 948.987 kb on - strand, within MMP_RS04975 at 949.010 kb on - strand, within MMP_RS04975 at 949.022 kb on - strand, within MMP_RS04975 at 949.039 kb on - strand, within MMP_RS04975 at 949.040 kb on + strand, within MMP_RS04975 at 949.040 kb on + strand, within MMP_RS04975 at 949.048 kb on + strand, within MMP_RS04975 at 949.048 kb on + strand, within MMP_RS04975 at 949.048 kb on + strand, within MMP_RS04975 at 949.093 kb on - strand, within MMP_RS04975 at 949.097 kb on + strand, within MMP_RS04975 at 949.188 kb on - strand at 949.225 kb on - strand at 949.225 kb on - strand at 949.225 kb on - strand at 949.225 kb on - strand at 949.225 kb on - strand at 949.225 kb on - strand at 949.226 kb on + strand at 949.231 kb on - strand at 949.232 kb on + strand at 949.239 kb on + strand at 949.313 kb on - strand at 949.313 kb on - strand at 949.314 kb on + strand at 949.335 kb on - strand at 949.336 kb on + strand at 949.382 kb on + strand at 949.384 kb on - strand at 949.384 kb on - strand at 949.384 kb on - strand at 949.384 kb on - strand at 949.384 kb on - strand at 949.385 kb on + strand at 949.385 kb on + strand at 949.385 kb on + strand at 949.385 kb on + strand at 949.386 kb on - strand at 949.387 kb on + strand at 949.387 kb on + strand at 949.387 kb on + strand at 949.387 kb on + strand at 949.387 kb on + strand at 949.387 kb on + strand at 949.390 kb on - strand at 949.420 kb on - strand at 949.431 kb on - strand at 949.445 kb on - strand at 949.460 kb on - strand at 949.491 kb on - strand at 949.491 kb on - strand at 949.493 kb on - strand at 949.493 kb on - strand at 949.494 kb on + strand at 949.494 kb on + strand at 949.494 kb on + strand at 949.494 kb on + strand at 949.494 kb on + strand at 949.497 kb on + strand at 949.498 kb on - strand at 949.498 kb on - strand at 949.498 kb on - strand at 949.499 kb on + strand at 949.499 kb on + strand at 949.499 kb on + strand at 949.499 kb on + strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.501 kb on - strand at 949.502 kb on + strand at 949.502 kb on + strand at 949.502 kb on + strand at 949.502 kb on + strand at 949.502 kb on + strand at 949.502 kb on + strand at 949.502 kb on + strand at 949.518 kb on + strand at 949.526 kb on - strand at 949.526 kb on - strand at 949.527 kb on + strand at 949.545 kb on - strand at 949.546 kb on + strand at 949.622 kb on + strand at 949.679 kb on - strand at 949.718 kb on - strand at 949.729 kb on - strand at 949.843 kb on - strand, within MMP_RS04980 at 949.889 kb on - strand, within MMP_RS04980 at 949.890 kb on + strand, within MMP_RS04980 at 949.891 kb on - strand, within MMP_RS04980 at 949.891 kb on - strand, within MMP_RS04980 at 949.901 kb on + strand, within MMP_RS04980 at 950.060 kb on - strand, within MMP_RS04980 at 950.061 kb on + strand, within MMP_RS04980 at 950.092 kb on - strand, within MMP_RS04980 at 950.158 kb on + strand, within MMP_RS04980 at 950.239 kb on - strand, within MMP_RS04980
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nitrofurantoin 0.25 mM remove 947,273 - -0.2 947,293 - +0.5 947,392 + MMP_RS04970 0.13 +0.1 947,434 + MMP_RS04970 0.18 +0.5 947,488 - MMP_RS04970 0.24 +0.2 947,635 - MMP_RS04970 0.40 -1.8 947,653 - MMP_RS04970 0.42 -0.9 947,674 - MMP_RS04970 0.44 -0.5 947,675 + MMP_RS04970 0.44 -0.7 947,675 + MMP_RS04970 0.44 -0.3 947,694 - MMP_RS04970 0.46 +0.9 947,695 + MMP_RS04970 0.47 +0.7 947,706 - MMP_RS04970 0.48 +0.1 947,706 - MMP_RS04970 0.48 -0.6 947,708 - MMP_RS04970 0.48 +0.3 947,708 - MMP_RS04970 0.48 +0.5 947,708 - MMP_RS04970 0.48 -0.2 947,786 + MMP_RS04970 0.57 -0.7 947,828 + MMP_RS04970 0.61 +0.0 947,847 - MMP_RS04970 0.63 -0.0 947,847 - MMP_RS04970 0.63 -1.0 947,847 - MMP_RS04970 0.63 -0.3 947,847 - MMP_RS04970 0.63 +0.2 947,863 - MMP_RS04970 0.65 +1.1 947,863 - MMP_RS04970 0.65 -0.4 947,864 + MMP_RS04970 0.65 -0.0 947,866 - MMP_RS04970 0.65 -0.3 947,883 + MMP_RS04970 0.67 -0.1 947,925 - MMP_RS04970 0.72 -0.6 947,930 + MMP_RS04970 0.73 -1.1 948,172 - -0.6 948,202 - -0.6 948,203 + -1.1 948,220 + -0.2 948,221 - +0.9 948,223 - +1.3 948,223 - +0.4 948,223 - -0.4 948,228 - +0.1 948,228 - +0.7 948,229 + +1.7 948,272 + +0.2 948,305 + -0.1 948,461 - MMP_RS04975 0.20 +1.3 948,602 - MMP_RS04975 0.34 -0.5 948,602 - MMP_RS04975 0.34 +0.1 948,604 - MMP_RS04975 0.34 -0.5 948,752 - MMP_RS04975 0.49 -0.2 948,791 - MMP_RS04975 0.52 -0.7 948,791 - MMP_RS04975 0.52 +3.5 948,792 + MMP_RS04975 0.52 -0.3 948,798 - MMP_RS04975 0.53 +0.7 948,798 - MMP_RS04975 0.53 +0.8 948,798 - MMP_RS04975 0.53 -0.1 948,799 + MMP_RS04975 0.53 -0.3 948,799 + MMP_RS04975 0.53 +0.6 948,799 + MMP_RS04975 0.53 +0.4 948,803 - MMP_RS04975 0.54 +0.3 948,803 - MMP_RS04975 0.54 +1.0 948,803 - MMP_RS04975 0.54 +2.0 948,803 - MMP_RS04975 0.54 +0.5 948,804 + MMP_RS04975 0.54 +0.6 948,806 + MMP_RS04975 0.54 +1.8 948,806 + MMP_RS04975 0.54 +1.4 948,806 + MMP_RS04975 0.54 +1.0 948,826 - MMP_RS04975 0.56 -0.6 948,831 - MMP_RS04975 0.56 -1.1 948,831 - MMP_RS04975 0.56 +0.3 948,832 + MMP_RS04975 0.56 +1.2 948,840 + MMP_RS04975 0.57 +0.0 948,904 + MMP_RS04975 0.64 -0.1 948,904 + MMP_RS04975 0.64 -0.3 948,904 + MMP_RS04975 0.64 +0.0 948,928 + MMP_RS04975 0.66 -0.2 948,938 - MMP_RS04975 0.67 +0.5 948,939 + MMP_RS04975 0.67 +2.0 948,943 - MMP_RS04975 0.67 +0.2 948,987 - MMP_RS04975 0.72 -0.4 949,010 - MMP_RS04975 0.74 -0.2 949,022 - MMP_RS04975 0.75 -0.6 949,039 - MMP_RS04975 0.77 +1.7 949,040 + MMP_RS04975 0.77 -0.6 949,040 + MMP_RS04975 0.77 -0.7 949,048 + MMP_RS04975 0.78 +1.7 949,048 + MMP_RS04975 0.78 +1.7 949,048 + MMP_RS04975 0.78 +0.8 949,093 - MMP_RS04975 0.82 +1.4 949,097 + MMP_RS04975 0.83 +0.2 949,188 - +0.5 949,225 - +0.5 949,225 - +1.9 949,225 - +0.7 949,225 - -0.7 949,225 - +0.6 949,225 - +0.5 949,226 + -0.6 949,231 - -0.7 949,232 + -0.4 949,239 + +1.0 949,313 - -0.1 949,313 - -0.4 949,314 + -1.3 949,335 - +0.8 949,336 + -0.2 949,382 + +0.8 949,384 - +0.1 949,384 - -0.7 949,384 - +1.3 949,384 - -0.3 949,384 - +1.7 949,385 + -0.2 949,385 + -1.1 949,385 + -0.2 949,385 + +1.1 949,386 - -0.0 949,387 + -0.4 949,387 + -0.0 949,387 + -0.0 949,387 + +0.7 949,387 + +0.5 949,387 + +0.6 949,390 - -0.6 949,420 - +0.1 949,431 - -1.9 949,445 - -0.2 949,460 - +0.3 949,491 - +1.1 949,491 - +0.6 949,493 - +0.2 949,493 - +0.7 949,494 + -0.6 949,494 + -1.0 949,494 + -0.6 949,494 + -0.9 949,494 + +1.5 949,497 + -1.1 949,498 - +0.2 949,498 - +0.3 949,498 - -0.3 949,499 + -0.2 949,499 + -0.3 949,499 + +1.3 949,499 + +0.8 949,501 - +0.1 949,501 - +0.9 949,501 - +0.6 949,501 - -2.2 949,501 - -0.2 949,501 - +0.3 949,501 - +0.6 949,501 - -0.2 949,501 - +0.9 949,502 + -0.6 949,502 + +0.1 949,502 + -0.0 949,502 + -0.5 949,502 + +1.1 949,502 + -0.1 949,502 + -0.7 949,518 + +0.7 949,526 - -0.1 949,526 - +0.1 949,527 + -0.2 949,545 - +0.4 949,546 + +2.1 949,622 + +0.7 949,679 - +0.4 949,718 - -0.3 949,729 - +1.8 949,843 - MMP_RS04980 0.15 +0.3 949,889 - MMP_RS04980 0.19 +0.4 949,890 + MMP_RS04980 0.19 -0.7 949,891 - MMP_RS04980 0.19 +0.5 949,891 - MMP_RS04980 0.19 +1.4 949,901 + MMP_RS04980 0.19 +1.0 950,060 - MMP_RS04980 0.30 +0.1 950,061 + MMP_RS04980 0.30 +0.3 950,092 - MMP_RS04980 0.32 -0.4 950,158 + MMP_RS04980 0.36 -0.8 950,239 - MMP_RS04980 0.42 +0.6
Or see this region's nucleotide sequence