Experiment: Formate with Nitrofurantoin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03545 and MMP_RS03550 are separated by 95 nucleotides MMP_RS03550 and MMP_RS03555 are separated by 122 nucleotides MMP_RS03555 and MMP_RS03560 are separated by 102 nucleotides
MMP_RS03545: MMP_RS03545 - ATP-binding protein, at 663,550 to 664,947
_RS03545
MMP_RS03550: MMP_RS03550 - DUF2124 domain-containing protein, at 665,043 to 665,519
_RS03550
MMP_RS03555: MMP_RS03555 - transcriptional regulator, at 665,642 to 666,622
_RS03555
MMP_RS03560: MMP_RS03560 - Na+/H+ antiporter NhaC family protein, at 666,725 to 668,302
_RS03560
Position (kb) 665
666
667 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 664.683 kb on + strand, within MMP_RS03545 at 664.726 kb on - strand, within MMP_RS03545 at 664.726 kb on - strand, within MMP_RS03545 at 664.726 kb on - strand, within MMP_RS03545 at 664.728 kb on - strand, within MMP_RS03545 at 664.751 kb on - strand, within MMP_RS03545 at 664.752 kb on + strand, within MMP_RS03545 at 664.809 kb on - strand at 664.855 kb on - strand at 664.859 kb on + strand at 664.942 kb on - strand at 664.953 kb on - strand at 664.982 kb on + strand at 664.984 kb on - strand at 665.278 kb on + strand, within MMP_RS03550 at 665.353 kb on - strand, within MMP_RS03550 at 665.478 kb on - strand at 665.546 kb on + strand at 665.552 kb on - strand at 665.552 kb on - strand at 665.553 kb on + strand at 665.553 kb on + strand at 665.553 kb on + strand at 665.556 kb on + strand at 665.556 kb on + strand at 665.578 kb on + strand at 665.754 kb on - strand, within MMP_RS03555 at 665.785 kb on + strand, within MMP_RS03555 at 665.785 kb on + strand, within MMP_RS03555 at 665.788 kb on - strand, within MMP_RS03555 at 666.107 kb on - strand, within MMP_RS03555 at 666.165 kb on - strand, within MMP_RS03555 at 666.166 kb on + strand, within MMP_RS03555 at 666.166 kb on + strand, within MMP_RS03555 at 666.166 kb on + strand, within MMP_RS03555 at 666.166 kb on + strand, within MMP_RS03555 at 666.397 kb on - strand, within MMP_RS03555 at 666.404 kb on + strand, within MMP_RS03555 at 666.621 kb on + strand at 666.727 kb on + strand at 666.756 kb on - strand at 666.935 kb on + strand, within MMP_RS03560 at 666.935 kb on + strand, within MMP_RS03560 at 666.969 kb on - strand, within MMP_RS03560 at 666.969 kb on - strand, within MMP_RS03560 at 666.969 kb on - strand, within MMP_RS03560 at 666.970 kb on + strand, within MMP_RS03560 at 666.970 kb on + strand, within MMP_RS03560 at 666.970 kb on + strand, within MMP_RS03560 at 666.971 kb on - strand, within MMP_RS03560 at 666.972 kb on + strand, within MMP_RS03560 at 667.084 kb on - strand, within MMP_RS03560 at 667.085 kb on + strand, within MMP_RS03560 at 667.085 kb on + strand, within MMP_RS03560 at 667.112 kb on - strand, within MMP_RS03560 at 667.115 kb on - strand, within MMP_RS03560 at 667.124 kb on - strand, within MMP_RS03560 at 667.176 kb on - strand, within MMP_RS03560 at 667.226 kb on - strand, within MMP_RS03560 at 667.227 kb on + strand, within MMP_RS03560 at 667.228 kb on - strand, within MMP_RS03560 at 667.229 kb on + strand, within MMP_RS03560 at 667.237 kb on - strand, within MMP_RS03560 at 667.315 kb on - strand, within MMP_RS03560 at 667.321 kb on + strand, within MMP_RS03560 at 667.331 kb on + strand, within MMP_RS03560 at 667.337 kb on - strand, within MMP_RS03560 at 667.346 kb on - strand, within MMP_RS03560 at 667.466 kb on - strand, within MMP_RS03560
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nitrofurantoin 0.25 mM remove 664,683 + MMP_RS03545 0.81 +0.9 664,726 - MMP_RS03545 0.84 -0.0 664,726 - MMP_RS03545 0.84 +0.5 664,726 - MMP_RS03545 0.84 -0.2 664,728 - MMP_RS03545 0.84 +0.7 664,751 - MMP_RS03545 0.86 +0.6 664,752 + MMP_RS03545 0.86 -0.9 664,809 - +2.0 664,855 - +0.5 664,859 + -0.4 664,942 - +1.0 664,953 - +1.4 664,982 + -0.7 664,984 - +0.1 665,278 + MMP_RS03550 0.49 +0.2 665,353 - MMP_RS03550 0.65 +0.7 665,478 - -0.3 665,546 + -0.1 665,552 - -0.6 665,552 - -0.9 665,553 + -0.5 665,553 + -0.2 665,553 + +0.2 665,556 + +1.4 665,556 + +0.8 665,578 + -1.2 665,754 - MMP_RS03555 0.11 -1.5 665,785 + MMP_RS03555 0.15 -0.1 665,785 + MMP_RS03555 0.15 -1.3 665,788 - MMP_RS03555 0.15 -0.5 666,107 - MMP_RS03555 0.47 -2.1 666,165 - MMP_RS03555 0.53 -0.8 666,166 + MMP_RS03555 0.53 -1.3 666,166 + MMP_RS03555 0.53 -1.6 666,166 + MMP_RS03555 0.53 +1.8 666,166 + MMP_RS03555 0.53 -2.5 666,397 - MMP_RS03555 0.77 -0.3 666,404 + MMP_RS03555 0.78 -1.2 666,621 + -0.8 666,727 + +0.1 666,756 - -3.1 666,935 + MMP_RS03560 0.13 -1.5 666,935 + MMP_RS03560 0.13 -1.0 666,969 - MMP_RS03560 0.15 -0.0 666,969 - MMP_RS03560 0.15 +0.6 666,969 - MMP_RS03560 0.15 +2.4 666,970 + MMP_RS03560 0.16 +0.2 666,970 + MMP_RS03560 0.16 -0.3 666,970 + MMP_RS03560 0.16 -0.7 666,971 - MMP_RS03560 0.16 -1.4 666,972 + MMP_RS03560 0.16 -0.1 667,084 - MMP_RS03560 0.23 +0.1 667,085 + MMP_RS03560 0.23 -0.4 667,085 + MMP_RS03560 0.23 +0.5 667,112 - MMP_RS03560 0.25 -0.5 667,115 - MMP_RS03560 0.25 +2.2 667,124 - MMP_RS03560 0.25 -0.8 667,176 - MMP_RS03560 0.29 -1.2 667,226 - MMP_RS03560 0.32 -0.2 667,227 + MMP_RS03560 0.32 +0.5 667,228 - MMP_RS03560 0.32 -1.3 667,229 + MMP_RS03560 0.32 -1.0 667,237 - MMP_RS03560 0.32 -0.8 667,315 - MMP_RS03560 0.37 +1.0 667,321 + MMP_RS03560 0.38 -0.7 667,331 + MMP_RS03560 0.38 -1.1 667,337 - MMP_RS03560 0.39 -1.2 667,346 - MMP_RS03560 0.39 +0.9 667,466 - MMP_RS03560 0.47 -1.0
Or see this region's nucleotide sequence