Experiment: Formate with Nitrofurantoin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03245 and MMP_RS03250 are separated by 105 nucleotides MMP_RS03250 and MMP_RS03255 are separated by 10 nucleotides MMP_RS03255 and MMP_RS03260 are separated by 22 nucleotides MMP_RS03260 and MMP_RS03265 are separated by 19 nucleotides MMP_RS03265 and MMP_RS03270 are separated by 46 nucleotides
MMP_RS03245: MMP_RS03245 - DNA repair and recombination protein RadB, at 612,707 to 613,357
_RS03245
MMP_RS03250: MMP_RS03250 - MJ1244 family protein, at 613,463 to 613,792
_RS03250
MMP_RS03255: MMP_RS03255 - DUF134 domain-containing protein, at 613,803 to 614,198
_RS03255
MMP_RS03260: MMP_RS03260 - methyl coenzyme M reductase system component A2, at 614,221 to 615,816
_RS03260
MMP_RS03265: MMP_RS03265 - sugar phosphate isomerase/epimerase, at 615,836 to 616,645
_RS03265
MMP_RS03270: MMP_RS03270 - ADP-ribosylglycohydrolase family protein, at 616,692 to 617,600
_RS03270
Position (kb) 614
615
616 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 613.250 kb on + strand, within MMP_RS03245 at 613.253 kb on + strand, within MMP_RS03245 at 613.376 kb on - strand at 613.405 kb on - strand at 613.409 kb on - strand at 613.410 kb on + strand at 613.428 kb on + strand at 613.428 kb on + strand at 613.702 kb on + strand, within MMP_RS03250 at 613.740 kb on + strand, within MMP_RS03250 at 613.741 kb on - strand, within MMP_RS03250 at 613.742 kb on + strand, within MMP_RS03250 at 614.274 kb on + strand at 614.286 kb on + strand at 614.533 kb on + strand at 614.537 kb on + strand at 614.537 kb on + strand at 614.896 kb on + strand at 615.296 kb on - strand at 615.669 kb on - strand at 615.672 kb on + strand at 615.672 kb on + strand at 615.871 kb on - strand at 615.875 kb on - strand at 615.876 kb on + strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.880 kb on - strand at 615.884 kb on - strand at 615.913 kb on - strand at 615.915 kb on - strand at 615.915 kb on - strand at 615.916 kb on + strand at 615.916 kb on + strand at 615.916 kb on + strand at 615.917 kb on - strand, within MMP_RS03265 at 615.930 kb on + strand, within MMP_RS03265 at 615.958 kb on - strand, within MMP_RS03265 at 615.981 kb on + strand, within MMP_RS03265 at 616.193 kb on - strand, within MMP_RS03265 at 616.279 kb on - strand, within MMP_RS03265 at 616.280 kb on + strand, within MMP_RS03265 at 616.409 kb on + strand, within MMP_RS03265 at 616.409 kb on + strand, within MMP_RS03265 at 616.454 kb on - strand, within MMP_RS03265 at 616.454 kb on - strand, within MMP_RS03265 at 616.454 kb on - strand, within MMP_RS03265 at 616.455 kb on + strand, within MMP_RS03265 at 616.455 kb on + strand, within MMP_RS03265 at 616.455 kb on + strand, within MMP_RS03265 at 616.456 kb on - strand, within MMP_RS03265 at 616.457 kb on + strand, within MMP_RS03265 at 616.460 kb on - strand, within MMP_RS03265 at 616.460 kb on - strand, within MMP_RS03265 at 616.461 kb on + strand, within MMP_RS03265 at 616.466 kb on - strand, within MMP_RS03265 at 616.466 kb on - strand, within MMP_RS03265 at 616.496 kb on - strand, within MMP_RS03265 at 616.496 kb on - strand, within MMP_RS03265 at 616.497 kb on + strand, within MMP_RS03265 at 616.506 kb on + strand, within MMP_RS03265 at 616.581 kb on + strand at 616.581 kb on + strand at 616.582 kb on - strand at 616.582 kb on - strand at 616.583 kb on + strand at 616.587 kb on + strand at 616.606 kb on + strand at 616.656 kb on - strand at 616.656 kb on - strand at 616.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nitrofurantoin 0.25 mM remove 613,250 + MMP_RS03245 0.83 +1.8 613,253 + MMP_RS03245 0.84 -0.3 613,376 - +0.6 613,405 - +0.2 613,409 - -3.0 613,410 + -3.1 613,428 + -1.7 613,428 + -4.6 613,702 + MMP_RS03250 0.72 -2.0 613,740 + MMP_RS03250 0.84 -3.0 613,741 - MMP_RS03250 0.84 -4.5 613,742 + MMP_RS03250 0.85 -2.0 614,274 + -2.1 614,286 + -4.0 614,533 + -0.9 614,537 + -2.2 614,537 + -2.5 614,896 + -1.6 615,296 - -2.9 615,669 - -2.0 615,672 + -2.8 615,672 + -2.1 615,871 - +0.8 615,875 - -0.4 615,876 + -0.3 615,880 - -0.7 615,880 - -0.7 615,880 - +3.4 615,884 - -1.3 615,913 - -0.9 615,915 - +0.4 615,915 - +1.0 615,916 + -0.3 615,916 + +0.6 615,916 + -0.2 615,917 - MMP_RS03265 0.10 +0.7 615,930 + MMP_RS03265 0.12 -0.9 615,958 - MMP_RS03265 0.15 -0.7 615,981 + MMP_RS03265 0.18 -0.6 616,193 - MMP_RS03265 0.44 +1.0 616,279 - MMP_RS03265 0.55 -1.0 616,280 + MMP_RS03265 0.55 -1.5 616,409 + MMP_RS03265 0.71 +0.2 616,409 + MMP_RS03265 0.71 -1.6 616,454 - MMP_RS03265 0.76 -0.7 616,454 - MMP_RS03265 0.76 -0.9 616,454 - MMP_RS03265 0.76 +1.0 616,455 + MMP_RS03265 0.76 +0.4 616,455 + MMP_RS03265 0.76 -0.2 616,455 + MMP_RS03265 0.76 +0.5 616,456 - MMP_RS03265 0.77 +0.7 616,457 + MMP_RS03265 0.77 -0.0 616,460 - MMP_RS03265 0.77 -0.9 616,460 - MMP_RS03265 0.77 -0.8 616,461 + MMP_RS03265 0.77 -0.9 616,466 - MMP_RS03265 0.78 +0.0 616,466 - MMP_RS03265 0.78 -0.0 616,496 - MMP_RS03265 0.81 +0.1 616,496 - MMP_RS03265 0.81 -0.7 616,497 + MMP_RS03265 0.82 -1.0 616,506 + MMP_RS03265 0.83 -0.6 616,581 + +0.1 616,581 + +1.0 616,582 - -0.0 616,582 - +1.4 616,583 + -0.4 616,587 + -0.9 616,606 + -0.9 616,656 - -0.2 616,656 - -1.1 616,672 - +0.0
Or see this region's nucleotide sequence