Experiment: Formate with Nitrofurantoin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01020 and MMP_RS01025 are separated by 120 nucleotides MMP_RS01025 and MMP_RS01030 are separated by 108 nucleotides MMP_RS01030 and MMP_RS01035 are separated by 14 nucleotides MMP_RS01035 and MMP_RS01040 are separated by 69 nucleotides
MMP_RS01020: MMP_RS01020 - 3 4-dihydroxy-2-butanone-4-phosphate synthase, at 192,407 to 193,150
_RS01020
MMP_RS01025: MMP_RS01025 - DUF120 domain-containing protein, at 193,271 to 193,654
_RS01025
MMP_RS01030: MMP_RS01030 - MTAP family purine nucleoside phosphorylase, at 193,763 to 194,524
_RS01030
MMP_RS01035: MMP_RS01035 - threonylcarbamoyl-AMP synthase, at 194,539 to 195,159
_RS01035
MMP_RS01040: MMP_RS01040 - phosphomethylpyrimidine synthase, at 195,229 to 196,509
_RS01040
Position (kb)
193
194
195 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 193.106 kb on - strand at 193.107 kb on + strand at 193.165 kb on + strand at 193.170 kb on - strand at 193.193 kb on + strand at 193.198 kb on - strand at 193.198 kb on - strand at 193.198 kb on - strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.199 kb on + strand at 193.200 kb on - strand at 193.200 kb on - strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.201 kb on + strand at 193.203 kb on + strand at 193.229 kb on - strand at 193.305 kb on + strand at 193.431 kb on + strand at 193.672 kb on - strand at 193.672 kb on - strand at 193.674 kb on - strand at 193.677 kb on + strand at 193.677 kb on + strand at 193.677 kb on + strand at 193.698 kb on + strand at 193.711 kb on + strand at 193.714 kb on - strand at 193.740 kb on + strand at 193.760 kb on - strand at 193.857 kb on - strand, within MMP_RS01030 at 193.989 kb on - strand, within MMP_RS01030 at 193.989 kb on - strand, within MMP_RS01030 at 194.124 kb on - strand, within MMP_RS01030 at 194.212 kb on + strand, within MMP_RS01030 at 194.407 kb on + strand, within MMP_RS01030 at 194.458 kb on - strand at 194.918 kb on - strand at 195.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nitrofurantoin 0.25 mM remove 193,106 - -2.0 193,107 + -2.9 193,165 + -1.2 193,170 - -1.5 193,193 + -1.5 193,198 - -0.6 193,198 - -1.1 193,198 - +1.5 193,199 + +0.0 193,199 + -0.3 193,199 + +1.0 193,199 + +0.7 193,199 + +1.4 193,200 - -0.8 193,200 - -0.3 193,201 + +0.1 193,201 + +0.2 193,201 + +0.3 193,201 + +1.6 193,201 + -0.1 193,201 + -0.3 193,203 + +1.2 193,229 - +0.4 193,305 + -3.3 193,431 + -0.5 193,672 - -2.9 193,672 - -0.3 193,674 - -2.3 193,677 + -2.8 193,677 + -4.0 193,677 + +1.2 193,698 + -0.4 193,711 + -0.9 193,714 - -0.5 193,740 + -0.5 193,760 - +0.8 193,857 - MMP_RS01030 0.12 -1.1 193,989 - MMP_RS01030 0.30 -0.5 193,989 - MMP_RS01030 0.30 -1.2 194,124 - MMP_RS01030 0.47 -1.0 194,212 + MMP_RS01030 0.59 -1.4 194,407 + MMP_RS01030 0.85 -0.5 194,458 - +1.0 194,918 - -2.9 195,337 + -1.8
Or see this region's nucleotide sequence