Experiment: Formate with Nitrofurantoin 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00310 and MMP_RS00315 are separated by 130 nucleotides MMP_RS00315 and MMP_RS00320 overlap by 4 nucleotides MMP_RS00320 and MMP_RS00325 are separated by 13 nucleotides
MMP_RS00310: MMP_RS00310 - CBS domain-containing protein, at 67,114 to 68,025
_RS00310
MMP_RS00315: MMP_RS00315 - Nif3-like dinuclear metal center hexameric protein, at 68,156 to 68,896
_RS00315
MMP_RS00320: MMP_RS00320 - class I SAM-dependent methyltransferase, at 68,893 to 69,501
_RS00320
MMP_RS00325: MMP_RS00325 - RNA-binding protein, at 69,515 to 69,994
_RS00325
Position (kb) 68
69 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 67.162 kb on - strand at 67.163 kb on + strand at 67.174 kb on + strand at 67.269 kb on + strand, within MMP_RS00310 at 67.295 kb on - strand, within MMP_RS00310 at 67.297 kb on - strand, within MMP_RS00310 at 67.369 kb on - strand, within MMP_RS00310 at 67.370 kb on + strand, within MMP_RS00310 at 67.439 kb on + strand, within MMP_RS00310 at 67.476 kb on - strand, within MMP_RS00310 at 67.476 kb on - strand, within MMP_RS00310 at 67.476 kb on - strand, within MMP_RS00310 at 67.476 kb on - strand, within MMP_RS00310 at 67.476 kb on - strand, within MMP_RS00310 at 67.477 kb on + strand, within MMP_RS00310 at 67.478 kb on - strand, within MMP_RS00310 at 67.478 kb on - strand, within MMP_RS00310 at 67.478 kb on - strand, within MMP_RS00310 at 67.479 kb on + strand, within MMP_RS00310 at 67.479 kb on + strand, within MMP_RS00310 at 67.479 kb on + strand, within MMP_RS00310 at 67.496 kb on + strand, within MMP_RS00310 at 67.544 kb on + strand, within MMP_RS00310 at 67.568 kb on + strand, within MMP_RS00310 at 67.568 kb on + strand, within MMP_RS00310 at 67.569 kb on - strand, within MMP_RS00310 at 67.570 kb on + strand, within MMP_RS00310 at 67.570 kb on + strand, within MMP_RS00310 at 67.815 kb on + strand, within MMP_RS00310 at 67.834 kb on + strand, within MMP_RS00310 at 67.883 kb on + strand, within MMP_RS00310 at 67.996 kb on - strand at 68.011 kb on - strand at 68.011 kb on - strand at 68.017 kb on + strand at 68.017 kb on + strand at 68.026 kb on + strand at 68.049 kb on - strand at 68.060 kb on - strand at 68.074 kb on - strand at 68.074 kb on - strand at 68.074 kb on - strand at 68.074 kb on - strand at 68.075 kb on + strand at 68.076 kb on - strand at 68.076 kb on - strand at 68.076 kb on - strand at 68.076 kb on - strand at 68.077 kb on + strand at 68.079 kb on + strand at 68.099 kb on - strand at 68.103 kb on + strand at 68.109 kb on + strand at 68.198 kb on - strand at 68.202 kb on - strand at 68.271 kb on + strand, within MMP_RS00315 at 68.339 kb on - strand, within MMP_RS00315 at 68.343 kb on - strand, within MMP_RS00315 at 68.343 kb on - strand, within MMP_RS00315 at 68.343 kb on - strand, within MMP_RS00315 at 68.613 kb on - strand, within MMP_RS00315 at 68.711 kb on - strand, within MMP_RS00315 at 68.711 kb on - strand, within MMP_RS00315 at 68.754 kb on + strand, within MMP_RS00315 at 69.287 kb on + strand, within MMP_RS00320 at 69.302 kb on + strand, within MMP_RS00320 at 69.446 kb on - strand at 69.447 kb on + strand at 69.448 kb on - strand at 69.454 kb on + strand at 69.749 kb on - strand, within MMP_RS00325 at 69.750 kb on + strand, within MMP_RS00325
Per-strain Table
Position Strand Gene LocusTag Fraction Formate with Nitrofurantoin 0.25 mM remove 67,162 - +0.1 67,163 + -0.6 67,174 + -1.1 67,269 + MMP_RS00310 0.17 +0.4 67,295 - MMP_RS00310 0.20 +0.4 67,297 - MMP_RS00310 0.20 +1.0 67,369 - MMP_RS00310 0.28 -2.0 67,370 + MMP_RS00310 0.28 -0.3 67,439 + MMP_RS00310 0.36 +0.8 67,476 - MMP_RS00310 0.40 -0.5 67,476 - MMP_RS00310 0.40 -1.5 67,476 - MMP_RS00310 0.40 -0.2 67,476 - MMP_RS00310 0.40 -0.8 67,476 - MMP_RS00310 0.40 -0.1 67,477 + MMP_RS00310 0.40 +0.6 67,478 - MMP_RS00310 0.40 -0.7 67,478 - MMP_RS00310 0.40 -0.4 67,478 - MMP_RS00310 0.40 -0.5 67,479 + MMP_RS00310 0.40 +0.8 67,479 + MMP_RS00310 0.40 -0.8 67,479 + MMP_RS00310 0.40 -0.3 67,496 + MMP_RS00310 0.42 +0.5 67,544 + MMP_RS00310 0.47 -0.9 67,568 + MMP_RS00310 0.50 -0.5 67,568 + MMP_RS00310 0.50 -0.4 67,569 - MMP_RS00310 0.50 -1.8 67,570 + MMP_RS00310 0.50 -0.3 67,570 + MMP_RS00310 0.50 -1.6 67,815 + MMP_RS00310 0.77 -0.9 67,834 + MMP_RS00310 0.79 -1.1 67,883 + MMP_RS00310 0.84 -0.3 67,996 - -1.5 68,011 - +0.2 68,011 - +1.2 68,017 + +1.2 68,017 + -0.1 68,026 + +0.0 68,049 - +0.5 68,060 - -0.5 68,074 - +0.5 68,074 - -0.2 68,074 - +0.4 68,074 - -0.6 68,075 + +0.5 68,076 - +0.2 68,076 - -0.9 68,076 - -0.2 68,076 - -0.8 68,077 + +0.1 68,079 + -0.1 68,099 - +0.6 68,103 + -1.3 68,109 + -0.3 68,198 - +0.6 68,202 - -2.0 68,271 + MMP_RS00315 0.16 -1.8 68,339 - MMP_RS00315 0.25 -1.0 68,343 - MMP_RS00315 0.25 -0.2 68,343 - MMP_RS00315 0.25 +0.0 68,343 - MMP_RS00315 0.25 -0.2 68,613 - MMP_RS00315 0.62 -1.3 68,711 - MMP_RS00315 0.75 -1.6 68,711 - MMP_RS00315 0.75 -0.5 68,754 + MMP_RS00315 0.81 -1.1 69,287 + MMP_RS00320 0.65 -0.3 69,302 + MMP_RS00320 0.67 -1.7 69,446 - +0.2 69,447 + -1.2 69,448 - -0.5 69,454 + -1.1 69,749 - MMP_RS00325 0.49 -0.7 69,750 + MMP_RS00325 0.49 +3.1
Or see this region's nucleotide sequence