Experiment: LB_Anaerobic_18HR_ZN20A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_04860 and CSW01_04865 are separated by 6 nucleotides CSW01_04865 and CSW01_04870 are separated by 2 nucleotides CSW01_04870 and CSW01_04875 are separated by 68 nucleotides CSW01_04875 and CSW01_04880 are separated by 5 nucleotides CSW01_04880 and CSW01_04885 are separated by 176 nucleotides
CSW01_04860: CSW01_04860 - penicillin-binding protein 2, at 1,012,954 to 1,014,870
_04860
CSW01_04865: CSW01_04865 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,014,877 to 1,015,347
_04865
CSW01_04870: CSW01_04870 - ribosome silencing factor, at 1,015,350 to 1,015,667
_04870
CSW01_04875: CSW01_04875 - DNA polymerase III subunit delta, at 1,015,736 to 1,016,764
_04875
CSW01_04880: CSW01_04880 - luciferase, at 1,016,770 to 1,017,384
_04880
CSW01_04885: CSW01_04885 - leucine--tRNA ligase, at 1,017,561 to 1,020,137
_04885
Position (kb)
1015
1016
1017 Strain fitness (log2 ratio)
-1
0
1 at 1015.066 kb on - strand, within CSW01_04865 at 1015.156 kb on - strand, within CSW01_04865 at 1015.156 kb on - strand, within CSW01_04865 at 1015.322 kb on - strand at 1015.488 kb on - strand, within CSW01_04870 at 1015.554 kb on - strand, within CSW01_04870 at 1015.684 kb on - strand at 1015.772 kb on - strand at 1017.534 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Anaerobic_18HR_ZN20A remove 1,015,066 - CSW01_04865 0.40 +0.5 1,015,156 - CSW01_04865 0.59 -0.3 1,015,156 - CSW01_04865 0.59 +0.3 1,015,322 - +0.1 1,015,488 - CSW01_04870 0.43 -0.1 1,015,554 - CSW01_04870 0.64 +1.1 1,015,684 - -0.2 1,015,772 - -0.5 1,017,534 - +0.4
Or see this region's nucleotide sequence