Experiment: m.b. Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2857 and Dshi_2858 are separated by 146 nucleotides Dshi_2858 and Dshi_2859 overlap by 4 nucleotides Dshi_2859 and Dshi_2860 overlap by 4 nucleotides Dshi_2860 and Dshi_2861 are separated by 92 nucleotides
Dshi_2857: Dshi_2857 - hypothetical protein (RefSeq), at 3,016,278 to 3,016,748
_2857
Dshi_2858: Dshi_2858 - purine nucleoside phosphorylase (RefSeq), at 3,016,895 to 3,017,599
_2858
Dshi_2859: Dshi_2859 - hypothetical protein (RefSeq), at 3,017,596 to 3,018,039
_2859
Dshi_2860: Dshi_2860 - hypothetical protein (RefSeq), at 3,018,036 to 3,018,221
_2860
Dshi_2861: Dshi_2861 - succinate dehydrogenase and fumarate reductase iron-sulfur protein (RefSeq), at 3,018,314 to 3,019,093
_2861
Position (kb)
3017
3018
3019 Strain fitness (log2 ratio)
-1
0
1 at 3016.622 kb on - strand, within Dshi_2857 at 3016.863 kb on + strand at 3016.871 kb on - strand at 3017.113 kb on - strand, within Dshi_2858 at 3017.144 kb on + strand, within Dshi_2858 at 3017.358 kb on - strand, within Dshi_2858 at 3017.530 kb on + strand at 3017.589 kb on - strand at 3017.644 kb on - strand, within Dshi_2859 at 3017.758 kb on - strand, within Dshi_2859 at 3018.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.004 mg/ml remove 3,016,622 - Dshi_2857 0.73 -0.6 3,016,863 + +0.8 3,016,871 - -0.1 3,017,113 - Dshi_2858 0.31 +1.2 3,017,144 + Dshi_2858 0.35 +0.3 3,017,358 - Dshi_2858 0.66 +0.7 3,017,530 + -0.2 3,017,589 - +0.3 3,017,644 - Dshi_2859 0.11 +0.8 3,017,758 - Dshi_2859 0.36 +0.8 3,018,308 - -0.1
Or see this region's nucleotide sequence