Experiment: m.b. Sisomicin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2134 and Dshi_2135 are separated by 122 nucleotides Dshi_2135 and Dshi_2136 are separated by 99 nucleotides Dshi_2136 and Dshi_2137 overlap by 4 nucleotides Dshi_2137 and Dshi_2138 are separated by 31 nucleotides
Dshi_2134: Dshi_2134 - BioY protein (RefSeq), at 2,264,629 to 2,265,222
_2134
Dshi_2135: Dshi_2135 - short-chain dehydrogenase/reductase SDR (RefSeq), at 2,265,345 to 2,266,010
_2135
Dshi_2136: Dshi_2136 - stationary-phase survival protein SurE (RefSeq), at 2,266,110 to 2,266,895
_2136
Dshi_2137: Dshi_2137 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 2,266,892 to 2,267,542
_2137
Dshi_2138: Dshi_2138 - peptidase M23B (RefSeq), at 2,267,574 to 2,268,803
_2138
Position (kb)
2266
2267 Strain fitness (log2 ratio)
-2
-1
0
1 at 2265.205 kb on - strand at 2265.323 kb on - strand at 2265.329 kb on + strand at 2265.455 kb on + strand, within Dshi_2135 at 2266.051 kb on + strand at 2266.089 kb on + strand at 2266.101 kb on + strand at 2266.106 kb on + strand at 2266.711 kb on - strand, within Dshi_2136
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.004 mg/ml remove 2,265,205 - +0.0 2,265,323 - -0.3 2,265,329 + -0.3 2,265,455 + Dshi_2135 0.17 -1.3 2,266,051 + -0.7 2,266,089 + -0.3 2,266,101 + -0.9 2,266,106 + -1.6 2,266,711 - Dshi_2136 0.76 -2.3
Or see this region's nucleotide sequence