Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2057 and Shew_2058 are separated by 159 nucleotides Shew_2058 and Shew_2059 are separated by 72 nucleotides Shew_2059 and Shew_R0071 are separated by 125 nucleotides Shew_R0071 and Shew_R0072 are separated by 31 nucleotides Shew_R0072 and Shew_R0073 are separated by 40 nucleotides Shew_R0073 and Shew_2060 are separated by 293 nucleotides
Shew_2057: Shew_2057 - diguanylate cyclase/phosphodiesterase with PAS/PAC and GAF sensor(s) (RefSeq), at 2,375,454 to 2,377,643
_2057
Shew_2058: Shew_2058 - hypothetical protein (RefSeq), at 2,377,803 to 2,378,090
_2058
Shew_2059: Shew_2059 - hypothetical protein (RefSeq), at 2,378,163 to 2,378,651
_2059
Shew_R0071: Shew_R0071 - tRNA-Tyr (RefSeq), at 2,378,777 to 2,378,861
_R0071
Shew_R0072: Shew_R0072 - tRNA-Tyr (RefSeq), at 2,378,893 to 2,378,977
_R0072
Shew_R0073: Shew_R0073 - tRNA-Tyr (RefSeq), at 2,379,018 to 2,379,102
_R0073
Shew_2060: Shew_2060 - pyridoxal-5'-phosphate-dependent enzyme, beta subunit (RefSeq), at 2,379,396 to 2,380,457
_2060
Position (kb)
2378
2379 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2377.802 kb on + strand at 2378.333 kb on - strand, within Shew_2059 at 2378.371 kb on - strand, within Shew_2059 at 2378.499 kb on - strand, within Shew_2059 at 2378.569 kb on - strand, within Shew_2059 at 2379.431 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 2,377,802 + -1.6 2,378,333 - Shew_2059 0.35 -2.8 2,378,371 - Shew_2059 0.43 -5.1 2,378,499 - Shew_2059 0.69 -3.4 2,378,569 - Shew_2059 0.83 -3.1 2,379,431 + -0.1
Or see this region's nucleotide sequence