Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1503 and Shew_1504 are separated by 131 nucleotides Shew_1504 and Shew_1505 are separated by 71 nucleotides Shew_1505 and Shew_1506 are separated by 232 nucleotides Shew_1506 and Shew_1507 are separated by 2 nucleotides
Shew_1503: Shew_1503 - oxidoreductase FAD-binding subunit (RefSeq), at 1,726,005 to 1,726,997
_1503
Shew_1504: Shew_1504 - GCN5-related N-acetyltransferase (RefSeq), at 1,727,129 to 1,727,560
_1504
Shew_1505: Shew_1505 - hypothetical protein (RefSeq), at 1,727,632 to 1,728,336
_1505
Shew_1506: Shew_1506 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 1,728,569 to 1,728,919
_1506
Shew_1507: Shew_1507 - 5-carboxymethyl-2-hydroxymuconate isomerase (RefSeq), at 1,728,922 to 1,729,269
_1507
Position (kb)
1727
1728
1729 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1727.004 kb on - strand at 1727.192 kb on + strand, within Shew_1504 at 1727.712 kb on - strand, within Shew_1505 at 1727.827 kb on - strand, within Shew_1505 at 1727.829 kb on + strand, within Shew_1505 at 1727.837 kb on - strand, within Shew_1505 at 1727.998 kb on - strand, within Shew_1505 at 1728.188 kb on - strand, within Shew_1505 at 1728.578 kb on + strand at 1728.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 1,727,004 - +0.4 1,727,192 + Shew_1504 0.15 +0.9 1,727,712 - Shew_1505 0.11 -0.4 1,727,827 - Shew_1505 0.28 -3.9 1,727,829 + Shew_1505 0.28 -3.7 1,727,837 - Shew_1505 0.29 -4.1 1,727,998 - Shew_1505 0.52 -3.1 1,728,188 - Shew_1505 0.79 -2.3 1,728,578 + -0.3 1,728,581 - +0.1
Or see this region's nucleotide sequence