Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliG and Shew_1368 overlap by 1 nucleotides Shew_1368 and fliI overlap by 29 nucleotides fliI and Shew_1370 are separated by 29 nucleotides Shew_1370 and Shew_1371 are separated by 380 nucleotides
Shew_1367: fliG - flagellar motor switch protein G (RefSeq), at 1,576,729 to 1,577,772
fliG
Shew_1368: Shew_1368 - flagellar assembly protein FliH (RefSeq), at 1,577,772 to 1,578,791
_1368
Shew_1369: fliI - flagellum-specific ATP synthase (RefSeq), at 1,578,763 to 1,580,103
fliI
Shew_1370: Shew_1370 - flagellar export protein FliJ (RefSeq), at 1,580,133 to 1,580,579
_1370
Shew_1371: Shew_1371 - flagellar hook-length control protein (RefSeq), at 1,580,960 to 1,582,666
_1371
Position (kb)
1578
1579
1580
1581 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1577.804 kb on + strand at 1577.956 kb on + strand, within Shew_1368 at 1577.981 kb on + strand, within Shew_1368 at 1578.043 kb on - strand, within Shew_1368 at 1578.061 kb on + strand, within Shew_1368 at 1578.565 kb on + strand, within Shew_1368 at 1578.565 kb on + strand, within Shew_1368 at 1578.920 kb on - strand, within fliI at 1579.284 kb on + strand, within fliI at 1579.292 kb on - strand, within fliI at 1579.866 kb on - strand, within fliI at 1580.254 kb on + strand, within Shew_1370 at 1580.598 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 1,577,804 + -5.9 1,577,956 + Shew_1368 0.18 -5.2 1,577,981 + Shew_1368 0.20 -6.2 1,578,043 - Shew_1368 0.27 -6.6 1,578,061 + Shew_1368 0.28 -6.5 1,578,565 + Shew_1368 0.78 -3.1 1,578,565 + Shew_1368 0.78 -2.2 1,578,920 - fliI Shew_1369 0.12 -7.2 1,579,284 + fliI Shew_1369 0.39 -4.9 1,579,292 - fliI Shew_1369 0.39 -4.6 1,579,866 - fliI Shew_1369 0.82 -6.9 1,580,254 + Shew_1370 0.27 -4.6 1,580,598 + +1.0
Or see this region's nucleotide sequence