Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1056 and Shew_1057 are separated by 128 nucleotides Shew_1057 and Shew_1058 are separated by 44 nucleotides Shew_1058 and Shew_1059 are separated by 266 nucleotides Shew_1059 and Shew_1060 are separated by 66 nucleotides
Shew_1056: Shew_1056 - DNA repair protein RecO (RefSeq), at 1,218,707 to 1,219,402
_1056
Shew_1057: Shew_1057 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 1,219,531 to 1,220,268
_1057
Shew_1058: Shew_1058 - holo-acyl-carrier-protein synthase (RefSeq), at 1,220,313 to 1,220,714
_1058
Shew_1059: Shew_1059 - hypothetical protein (RefSeq), at 1,220,981 to 1,221,523
_1059
Shew_1060: Shew_1060 - hypothetical protein (RefSeq), at 1,221,590 to 1,222,873
_1060
Position (kb)
1220
1221 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1219.380 kb on - strand at 1220.302 kb on + strand at 1220.419 kb on - strand, within Shew_1058 at 1220.421 kb on + strand, within Shew_1058 at 1220.576 kb on + strand, within Shew_1058 at 1220.644 kb on + strand, within Shew_1058 at 1221.033 kb on - strand at 1221.293 kb on - strand, within Shew_1059 at 1221.431 kb on - strand, within Shew_1059 at 1221.491 kb on + strand at 1221.602 kb on + strand at 1221.635 kb on - strand at 1221.673 kb on + strand at 1221.713 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 1,219,380 - +0.9 1,220,302 + +0.0 1,220,419 - Shew_1058 0.26 -5.6 1,220,421 + Shew_1058 0.27 -3.4 1,220,576 + Shew_1058 0.65 -3.8 1,220,644 + Shew_1058 0.82 -3.2 1,221,033 - -0.2 1,221,293 - Shew_1059 0.57 -0.5 1,221,431 - Shew_1059 0.83 -0.4 1,221,491 + -0.2 1,221,602 + -0.8 1,221,635 - -0.6 1,221,673 + +0.8 1,221,713 - -0.3
Or see this region's nucleotide sequence