Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0307 and Shew_0308 are separated by 8 nucleotides Shew_0308 and Shew_0309 are separated by 16 nucleotides Shew_0309 and Shew_0310 overlap by 1 nucleotides
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Shew_0308: Shew_0308 - hypothetical protein (RefSeq), at 334,532 to 335,239
_0308
Shew_0309: Shew_0309 - OmpA/MotB domain-containing protein (RefSeq), at 335,256 to 335,861
_0309
Shew_0310: Shew_0310 - TolC family type I secretion outer membrane protein (RefSeq), at 335,861 to 337,273
_0310
Position (kb)
334
335
336 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 333.753 kb on + strand, within Shew_0307 at 333.838 kb on + strand, within Shew_0307 at 334.028 kb on + strand, within Shew_0307 at 334.074 kb on + strand, within Shew_0307 at 334.405 kb on + strand at 334.409 kb on + strand at 334.498 kb on + strand at 334.669 kb on + strand, within Shew_0308 at 334.783 kb on - strand, within Shew_0308 at 334.911 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.940 kb on - strand, within Shew_0308 at 334.974 kb on + strand, within Shew_0308 at 335.049 kb on + strand, within Shew_0308 at 335.058 kb on - strand, within Shew_0308 at 335.173 kb on - strand at 335.173 kb on - strand at 335.394 kb on + strand, within Shew_0309 at 335.585 kb on + strand, within Shew_0309 at 335.873 kb on + strand at 335.894 kb on + strand at 335.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 333,753 + Shew_0307 0.60 -0.3 333,838 + Shew_0307 0.64 +0.1 334,028 + Shew_0307 0.74 +1.0 334,074 + Shew_0307 0.77 -0.4 334,405 + +0.1 334,409 + -1.1 334,498 + -0.5 334,669 + Shew_0308 0.19 -2.5 334,783 - Shew_0308 0.35 -1.4 334,911 - Shew_0308 0.54 -2.6 334,940 - Shew_0308 0.58 -3.3 334,940 - Shew_0308 0.58 -0.9 334,974 + Shew_0308 0.62 -2.4 335,049 + Shew_0308 0.73 -2.6 335,058 - Shew_0308 0.74 -2.1 335,173 - -3.5 335,173 - -2.2 335,394 + Shew_0309 0.23 -0.3 335,585 + Shew_0309 0.54 -0.1 335,873 + -3.1 335,894 + -2.4 335,954 - -1.0
Or see this region's nucleotide sequence