Experiment: Motility M4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0131 and Shew_0132 are separated by 107 nucleotides Shew_0132 and Shew_0133 overlap by 10 nucleotides Shew_0133 and Shew_0134 are separated by 47 nucleotides
Shew_0131: Shew_0131 - GCN5-related N-acetyltransferase (RefSeq), at 140,435 to 140,890
_0131
Shew_0132: Shew_0132 - TetR family transcriptional regulator (RefSeq), at 140,998 to 141,654
_0132
Shew_0133: Shew_0133 - phospholipid/glycerol acyltransferase (RefSeq), at 141,645 to 143,507
_0133
Shew_0134: Shew_0134 - tRNA 2-selenouridine synthase (RefSeq), at 143,555 to 144,673
_0134
Position (kb) 141
142
143
144 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 140.665 kb on + strand, within Shew_0131 at 140.756 kb on - strand, within Shew_0131 at 140.855 kb on + strand at 141.028 kb on - strand at 141.097 kb on - strand, within Shew_0132 at 141.099 kb on + strand, within Shew_0132 at 141.276 kb on - strand, within Shew_0132 at 141.342 kb on + strand, within Shew_0132 at 141.471 kb on + strand, within Shew_0132 at 141.536 kb on + strand, within Shew_0132 at 141.570 kb on + strand, within Shew_0132 at 141.583 kb on - strand, within Shew_0132 at 141.886 kb on + strand, within Shew_0133 at 141.997 kb on + strand, within Shew_0133 at 142.032 kb on - strand, within Shew_0133 at 142.069 kb on + strand, within Shew_0133 at 142.270 kb on - strand, within Shew_0133 at 142.528 kb on + strand, within Shew_0133 at 142.536 kb on - strand, within Shew_0133 at 142.548 kb on + strand, within Shew_0133 at 142.747 kb on - strand, within Shew_0133 at 142.766 kb on - strand, within Shew_0133 at 142.780 kb on - strand, within Shew_0133 at 142.859 kb on + strand, within Shew_0133 at 142.867 kb on - strand, within Shew_0133 at 142.944 kb on - strand, within Shew_0133 at 142.944 kb on - strand, within Shew_0133 at 142.944 kb on - strand, within Shew_0133 at 142.982 kb on - strand, within Shew_0133 at 142.996 kb on + strand, within Shew_0133 at 143.086 kb on - strand, within Shew_0133 at 143.105 kb on + strand, within Shew_0133 at 143.154 kb on - strand, within Shew_0133 at 143.350 kb on - strand at 143.382 kb on - strand at 143.386 kb on + strand at 143.601 kb on - strand at 143.635 kb on - strand at 143.704 kb on + strand, within Shew_0134 at 143.804 kb on - strand, within Shew_0134 at 143.973 kb on + strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.986 kb on - strand, within Shew_0134 at 144.048 kb on + strand, within Shew_0134 at 144.053 kb on + strand, within Shew_0134 at 144.255 kb on - strand, within Shew_0134 at 144.265 kb on + strand, within Shew_0134
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M4 remove 140,665 + Shew_0131 0.50 -0.9 140,756 - Shew_0131 0.70 -2.1 140,855 + +1.1 141,028 - +0.0 141,097 - Shew_0132 0.15 -2.3 141,099 + Shew_0132 0.15 +0.1 141,276 - Shew_0132 0.42 -0.1 141,342 + Shew_0132 0.52 +0.7 141,471 + Shew_0132 0.72 +0.0 141,536 + Shew_0132 0.82 -0.3 141,570 + Shew_0132 0.87 +0.3 141,583 - Shew_0132 0.89 +1.0 141,886 + Shew_0133 0.13 -0.1 141,997 + Shew_0133 0.19 -0.8 142,032 - Shew_0133 0.21 +1.0 142,069 + Shew_0133 0.23 +0.5 142,270 - Shew_0133 0.34 +0.1 142,528 + Shew_0133 0.47 +0.1 142,536 - Shew_0133 0.48 -1.7 142,548 + Shew_0133 0.48 -1.3 142,747 - Shew_0133 0.59 -0.2 142,766 - Shew_0133 0.60 -0.2 142,780 - Shew_0133 0.61 -0.5 142,859 + Shew_0133 0.65 -0.4 142,867 - Shew_0133 0.66 +0.6 142,944 - Shew_0133 0.70 +2.4 142,944 - Shew_0133 0.70 -2.8 142,944 - Shew_0133 0.70 +0.3 142,982 - Shew_0133 0.72 -2.1 142,996 + Shew_0133 0.73 -1.6 143,086 - Shew_0133 0.77 +0.8 143,105 + Shew_0133 0.78 +0.4 143,154 - Shew_0133 0.81 -1.3 143,350 - -0.4 143,382 - -0.1 143,386 + -1.5 143,601 - -0.4 143,635 - +0.2 143,704 + Shew_0134 0.13 -0.1 143,804 - Shew_0134 0.22 +0.3 143,973 + Shew_0134 0.37 -0.7 143,981 - Shew_0134 0.38 +1.2 143,981 - Shew_0134 0.38 -0.2 143,981 - Shew_0134 0.38 +0.2 143,981 - Shew_0134 0.38 +1.5 143,986 - Shew_0134 0.39 +0.4 144,048 + Shew_0134 0.44 -0.3 144,053 + Shew_0134 0.45 +0.1 144,255 - Shew_0134 0.63 +2.0 144,265 + Shew_0134 0.63 -0.5
Or see this region's nucleotide sequence