Experiment: m.b. Sisomicin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3815 and Dshi_3816 are separated by 2 nucleotides Dshi_3816 and Dshi_3817 are separated by 125 nucleotides Dshi_3817 and Dshi_3818 are separated by 17 nucleotides Dshi_3818 and Dshi_3819 overlap by 4 nucleotides
Dshi_3815: Dshi_3815 - monooxygenase FAD-binding (RefSeq), at 23,077 to 24,702
_3815
Dshi_3816: Dshi_3816 - hypothetical protein (RefSeq), at 24,705 to 24,890
_3816
Dshi_3817: Dshi_3817 - beta-ketoadipyl CoA thiolase (RefSeq), at 25,016 to 26,218
_3817
Dshi_3818: Dshi_3818 - phenylacetate-CoA oxygenase, PaaG subunit (RefSeq), at 26,236 to 27,213
_3818
Dshi_3819: Dshi_3819 - phenylacetate-CoA oxygenase, PaaH subunit (RefSeq), at 27,210 to 27,542
_3819
Position (kb)
25
26
27 Strain fitness (log2 ratio)
-1
0
1 at 24.135 kb on - strand, within Dshi_3815 at 24.137 kb on - strand, within Dshi_3815 at 24.139 kb on + strand, within Dshi_3815 at 24.157 kb on - strand, within Dshi_3815 at 24.165 kb on + strand, within Dshi_3815 at 24.165 kb on + strand, within Dshi_3815 at 24.173 kb on - strand, within Dshi_3815 at 24.259 kb on + strand, within Dshi_3815 at 24.267 kb on - strand, within Dshi_3815 at 24.439 kb on - strand, within Dshi_3815 at 24.632 kb on - strand at 24.721 kb on - strand at 24.721 kb on - strand at 25.144 kb on + strand, within Dshi_3817 at 25.153 kb on - strand, within Dshi_3817 at 25.313 kb on - strand, within Dshi_3817 at 25.409 kb on + strand, within Dshi_3817 at 25.410 kb on + strand, within Dshi_3817 at 25.430 kb on + strand, within Dshi_3817 at 25.695 kb on - strand, within Dshi_3817 at 25.928 kb on + strand, within Dshi_3817 at 25.978 kb on + strand, within Dshi_3817 at 26.002 kb on - strand, within Dshi_3817 at 26.019 kb on + strand, within Dshi_3817 at 26.111 kb on + strand at 26.144 kb on + strand at 26.196 kb on - strand at 26.212 kb on + strand at 26.325 kb on - strand at 26.365 kb on + strand, within Dshi_3818 at 26.532 kb on - strand, within Dshi_3818 at 26.561 kb on + strand, within Dshi_3818 at 26.611 kb on + strand, within Dshi_3818 at 26.619 kb on - strand, within Dshi_3818 at 26.649 kb on + strand, within Dshi_3818 at 26.722 kb on + strand, within Dshi_3818 at 26.770 kb on - strand, within Dshi_3818 at 26.823 kb on - strand, within Dshi_3818 at 26.874 kb on - strand, within Dshi_3818 at 26.880 kb on - strand, within Dshi_3818 at 26.956 kb on + strand, within Dshi_3818 at 26.963 kb on + strand, within Dshi_3818 at 26.997 kb on + strand, within Dshi_3818
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.002 mg/ml remove 24,135 - Dshi_3815 0.65 -0.1 24,137 - Dshi_3815 0.65 -0.0 24,139 + Dshi_3815 0.65 +0.2 24,157 - Dshi_3815 0.66 +0.0 24,165 + Dshi_3815 0.67 -0.7 24,165 + Dshi_3815 0.67 -1.3 24,173 - Dshi_3815 0.67 -0.3 24,259 + Dshi_3815 0.73 -0.2 24,267 - Dshi_3815 0.73 +0.2 24,439 - Dshi_3815 0.84 +0.4 24,632 - +0.0 24,721 - +0.5 24,721 - -0.2 25,144 + Dshi_3817 0.11 -0.4 25,153 - Dshi_3817 0.11 +0.5 25,313 - Dshi_3817 0.25 +0.5 25,409 + Dshi_3817 0.33 +0.7 25,410 + Dshi_3817 0.33 +0.5 25,430 + Dshi_3817 0.34 -0.4 25,695 - Dshi_3817 0.56 -0.5 25,928 + Dshi_3817 0.76 +0.6 25,978 + Dshi_3817 0.80 -0.4 26,002 - Dshi_3817 0.82 +0.2 26,019 + Dshi_3817 0.83 +0.5 26,111 + -0.2 26,144 + +0.9 26,196 - -0.6 26,212 + -0.2 26,325 - +0.8 26,365 + Dshi_3818 0.13 +0.6 26,532 - Dshi_3818 0.30 +0.2 26,561 + Dshi_3818 0.33 +0.8 26,611 + Dshi_3818 0.38 +0.3 26,619 - Dshi_3818 0.39 -0.3 26,649 + Dshi_3818 0.42 -0.5 26,722 + Dshi_3818 0.50 +0.7 26,770 - Dshi_3818 0.55 -0.4 26,823 - Dshi_3818 0.60 +0.3 26,874 - Dshi_3818 0.65 +0.6 26,880 - Dshi_3818 0.66 +0.5 26,956 + Dshi_3818 0.74 -0.6 26,963 + Dshi_3818 0.74 -0.5 26,997 + Dshi_3818 0.78 +0.8
Or see this region's nucleotide sequence