Experiment: m.b. Sisomicin 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0065 and Dshi_0066 are separated by 115 nucleotides Dshi_0066 and Dshi_0067 overlap by 4 nucleotides Dshi_0067 and Dshi_0068 are separated by 4 nucleotides Dshi_0068 and Dshi_0069 are separated by 76 nucleotides
Dshi_0065: Dshi_0065 - hypothetical protein (RefSeq), at 65,978 to 67,468
_0065
Dshi_0066: Dshi_0066 - short-chain dehydrogenase/reductase SDR (RefSeq), at 67,584 to 68,342
_0066
Dshi_0067: Dshi_0067 - Enoyl-CoA hydratase/isomerase (RefSeq), at 68,339 to 69,085
_0067
Dshi_0068: Dshi_0068 - lipoprotein, putative (RefSeq), at 69,090 to 69,674
_0068
Dshi_0069: Dshi_0069 - Auxin Efflux Carrier (RefSeq), at 69,751 to 70,632
_0069
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-2
-1
0
1 at 67.365 kb on + strand at 67.366 kb on + strand at 67.373 kb on - strand at 67.395 kb on - strand at 67.406 kb on - strand at 67.417 kb on - strand at 67.456 kb on + strand at 67.474 kb on - strand at 67.527 kb on - strand at 67.531 kb on + strand at 67.531 kb on + strand at 67.537 kb on - strand at 67.553 kb on - strand at 67.563 kb on + strand at 67.578 kb on - strand at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.748 kb on + strand, within Dshi_0066 at 67.810 kb on - strand at 67.810 kb on - strand, within Dshi_0066 at 67.851 kb on - strand, within Dshi_0066 at 67.920 kb on - strand, within Dshi_0066 at 67.931 kb on + strand, within Dshi_0066 at 68.208 kb on + strand, within Dshi_0066 at 68.251 kb on - strand, within Dshi_0066 at 68.393 kb on + strand at 68.398 kb on + strand at 68.533 kb on + strand, within Dshi_0067 at 68.585 kb on + strand, within Dshi_0067 at 68.594 kb on - strand, within Dshi_0067 at 68.595 kb on + strand, within Dshi_0067 at 68.600 kb on + strand, within Dshi_0067 at 68.603 kb on - strand, within Dshi_0067 at 68.603 kb on - strand, within Dshi_0067 at 68.772 kb on - strand, within Dshi_0067 at 68.935 kb on + strand, within Dshi_0067 at 69.411 kb on - strand, within Dshi_0068 at 69.776 kb on + strand at 69.810 kb on + strand at 69.813 kb on - strand at 69.825 kb on - strand at 69.855 kb on - strand, within Dshi_0069 at 69.960 kb on - strand, within Dshi_0069 at 70.010 kb on + strand, within Dshi_0069 at 70.064 kb on + strand, within Dshi_0069
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Sisomicin 0.002 mg/ml remove 67,365 + +0.4 67,366 + -0.0 67,373 - -0.4 67,395 - +0.5 67,406 - +0.5 67,417 - +0.0 67,456 + +0.2 67,474 - -0.2 67,527 - -0.4 67,531 + +0.6 67,531 + -0.5 67,537 - -0.2 67,553 - -0.6 67,563 + -0.3 67,578 - -0.7 67,748 + Dshi_0066 0.22 -0.4 67,748 + Dshi_0066 0.22 -0.5 67,748 + Dshi_0066 0.22 -1.9 67,810 - -0.9 67,810 - Dshi_0066 0.30 -1.3 67,851 - Dshi_0066 0.35 -0.4 67,920 - Dshi_0066 0.44 +0.1 67,931 + Dshi_0066 0.46 -0.2 68,208 + Dshi_0066 0.82 -1.0 68,251 - Dshi_0066 0.88 -0.7 68,393 + -0.3 68,398 + +0.1 68,533 + Dshi_0067 0.26 +0.2 68,585 + Dshi_0067 0.33 +1.1 68,594 - Dshi_0067 0.34 +1.6 68,595 + Dshi_0067 0.34 -0.6 68,600 + Dshi_0067 0.35 +0.7 68,603 - Dshi_0067 0.35 -0.2 68,603 - Dshi_0067 0.35 -0.4 68,772 - Dshi_0067 0.58 -0.5 68,935 + Dshi_0067 0.80 -0.1 69,411 - Dshi_0068 0.55 -1.6 69,776 + -2.2 69,810 + +0.6 69,813 - +0.6 69,825 - +0.7 69,855 - Dshi_0069 0.12 -0.0 69,960 - Dshi_0069 0.24 -1.3 70,010 + Dshi_0069 0.29 +0.0 70,064 + Dshi_0069 0.35 -0.2
Or see this region's nucleotide sequence