Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3382 and Rru_A3383 are separated by 158 nucleotides Rru_A3383 and Rru_A3384 overlap by 4 nucleotides Rru_A3384 and Rru_A3385 are separated by 120 nucleotides
Rru_A3382: Rru_A3382 - Cobalamin biosynthesis protein CbiB (NCBI), at 3,898,851 to 3,899,861
_A3382
Rru_A3383: Rru_A3383 - Cobalamin (vitamin B12) biosynthesis CbiG protein (NCBI), at 3,900,020 to 3,901,414
_A3383
Rru_A3384: Rru_A3384 - Precorrin-4 C11-methyltransferase region (NCBI), at 3,901,411 to 3,902,172
_A3384
Rru_A3385: Rru_A3385 - OmpA/MotB (NCBI), at 3,902,293 to 3,903,021
_A3385
Position (kb)
3900
3901
3902 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3899.106 kb on + strand, within Rru_A3382 at 3899.294 kb on + strand, within Rru_A3382 at 3899.294 kb on + strand, within Rru_A3382 at 3899.294 kb on + strand, within Rru_A3382 at 3899.295 kb on - strand, within Rru_A3382 at 3899.295 kb on - strand, within Rru_A3382 at 3899.296 kb on + strand, within Rru_A3382 at 3899.297 kb on - strand, within Rru_A3382 at 3899.779 kb on + strand at 3899.779 kb on + strand at 3899.779 kb on + strand at 3899.780 kb on - strand at 3899.780 kb on - strand at 3899.780 kb on - strand at 3899.780 kb on - strand at 3899.780 kb on - strand at 3899.930 kb on + strand at 3899.930 kb on + strand at 3899.960 kb on + strand at 3899.960 kb on + strand at 3899.961 kb on - strand at 3899.963 kb on - strand at 3899.963 kb on - strand at 3899.963 kb on - strand at 3899.963 kb on - strand at 3899.963 kb on - strand at 3900.448 kb on + strand, within Rru_A3383 at 3900.678 kb on + strand, within Rru_A3383 at 3900.679 kb on - strand, within Rru_A3383 at 3900.749 kb on + strand, within Rru_A3383 at 3900.750 kb on - strand, within Rru_A3383 at 3900.750 kb on - strand, within Rru_A3383 at 3900.750 kb on - strand, within Rru_A3383 at 3900.750 kb on - strand, within Rru_A3383 at 3901.580 kb on + strand, within Rru_A3384 at 3901.669 kb on + strand, within Rru_A3384 at 3901.669 kb on + strand, within Rru_A3384 at 3901.669 kb on + strand, within Rru_A3384 at 3901.720 kb on + strand, within Rru_A3384 at 3901.747 kb on + strand, within Rru_A3384 at 3901.821 kb on + strand, within Rru_A3384 at 3901.821 kb on + strand, within Rru_A3384 at 3901.822 kb on - strand, within Rru_A3384 at 3901.822 kb on - strand, within Rru_A3384 at 3901.822 kb on - strand, within Rru_A3384 at 3901.905 kb on - strand, within Rru_A3384 at 3901.905 kb on - strand, within Rru_A3384 at 3901.905 kb on - strand, within Rru_A3384 at 3901.951 kb on + strand, within Rru_A3384 at 3901.981 kb on + strand, within Rru_A3384 at 3902.128 kb on + strand at 3902.129 kb on - strand at 3902.129 kb on - strand at 3902.129 kb on - strand at 3902.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 3,899,106 + Rru_A3382 0.25 +0.0 3,899,294 + Rru_A3382 0.44 +0.0 3,899,294 + Rru_A3382 0.44 -2.5 3,899,294 + Rru_A3382 0.44 +0.0 3,899,295 - Rru_A3382 0.44 -1.0 3,899,295 - Rru_A3382 0.44 -2.7 3,899,296 + Rru_A3382 0.44 +0.0 3,899,297 - Rru_A3382 0.44 +0.0 3,899,779 + +0.0 3,899,779 + +0.0 3,899,779 + +0.0 3,899,780 - +0.0 3,899,780 - +0.0 3,899,780 - +0.0 3,899,780 - -3.1 3,899,780 - +0.0 3,899,930 + -2.5 3,899,930 + +0.0 3,899,960 + +0.0 3,899,960 + +0.0 3,899,961 - +0.0 3,899,963 - -2.3 3,899,963 - +0.0 3,899,963 - +0.0 3,899,963 - +0.0 3,899,963 - +0.0 3,900,448 + Rru_A3383 0.31 -2.8 3,900,678 + Rru_A3383 0.47 -4.0 3,900,679 - Rru_A3383 0.47 +0.0 3,900,749 + Rru_A3383 0.52 -3.4 3,900,750 - Rru_A3383 0.52 -2.3 3,900,750 - Rru_A3383 0.52 +0.0 3,900,750 - Rru_A3383 0.52 -2.0 3,900,750 - Rru_A3383 0.52 +0.0 3,901,580 + Rru_A3384 0.22 +0.0 3,901,669 + Rru_A3384 0.34 +0.0 3,901,669 + Rru_A3384 0.34 +0.0 3,901,669 + Rru_A3384 0.34 +0.0 3,901,720 + Rru_A3384 0.41 -1.7 3,901,747 + Rru_A3384 0.44 +0.0 3,901,821 + Rru_A3384 0.54 +0.0 3,901,821 + Rru_A3384 0.54 +0.0 3,901,822 - Rru_A3384 0.54 -1.9 3,901,822 - Rru_A3384 0.54 +0.0 3,901,822 - Rru_A3384 0.54 +0.0 3,901,905 - Rru_A3384 0.65 +0.0 3,901,905 - Rru_A3384 0.65 +0.0 3,901,905 - Rru_A3384 0.65 +0.0 3,901,951 + Rru_A3384 0.71 +0.0 3,901,981 + Rru_A3384 0.75 +0.0 3,902,128 + +0.0 3,902,129 - -1.0 3,902,129 - -1.8 3,902,129 - +0.0 3,902,228 - +0.0
Or see this region's nucleotide sequence