Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2501 and Rru_A2502 are separated by 146 nucleotides Rru_A2502 and Rru_A2503 overlap by 20 nucleotides Rru_A2503 and Rru_A2504 are separated by 272 nucleotides
Rru_A2501: Rru_A2501 - conserved hypothetical cytosolic protein (NCBI), at 2,908,139 to 2,908,660
_A2501
Rru_A2502: Rru_A2502 - Protein of unknown function DUF454 (NCBI), at 2,908,807 to 2,909,265
_A2502
Rru_A2503: Rru_A2503 - Hydroxypyruvate isomerase (NCBI), at 2,909,246 to 2,910,040
_A2503
Rru_A2504: Rru_A2504 - Oxygen-independent coproporphyrinogen III oxidase HemN (NCBI), at 2,910,313 to 2,911,722
_A2504
Position (kb)
2909
2910
2911 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2908.426 kb on - strand, within Rru_A2501 at 2908.546 kb on + strand, within Rru_A2501 at 2908.547 kb on - strand, within Rru_A2501 at 2908.748 kb on + strand at 2908.748 kb on + strand at 2908.748 kb on + strand at 2908.749 kb on - strand at 2908.749 kb on - strand at 2908.749 kb on - strand at 2908.749 kb on - strand at 2908.752 kb on + strand at 2908.753 kb on - strand at 2908.930 kb on + strand, within Rru_A2502 at 2908.931 kb on - strand, within Rru_A2502 at 2908.931 kb on - strand, within Rru_A2502 at 2909.097 kb on + strand, within Rru_A2502 at 2909.098 kb on - strand, within Rru_A2502 at 2909.098 kb on - strand, within Rru_A2502 at 2909.319 kb on + strand at 2909.320 kb on - strand at 2909.320 kb on - strand at 2909.320 kb on - strand at 2909.373 kb on + strand, within Rru_A2503 at 2909.373 kb on + strand, within Rru_A2503 at 2909.373 kb on + strand, within Rru_A2503 at 2909.373 kb on + strand, within Rru_A2503 at 2909.373 kb on + strand, within Rru_A2503 at 2909.374 kb on - strand, within Rru_A2503 at 2909.505 kb on + strand, within Rru_A2503 at 2909.505 kb on + strand, within Rru_A2503 at 2909.506 kb on - strand, within Rru_A2503 at 2909.506 kb on - strand, within Rru_A2503 at 2909.514 kb on + strand, within Rru_A2503 at 2909.515 kb on - strand, within Rru_A2503 at 2909.586 kb on + strand, within Rru_A2503 at 2909.606 kb on - strand, within Rru_A2503 at 2909.661 kb on + strand, within Rru_A2503 at 2909.661 kb on + strand, within Rru_A2503 at 2909.662 kb on - strand, within Rru_A2503 at 2909.662 kb on - strand, within Rru_A2503 at 2909.679 kb on + strand, within Rru_A2503 at 2909.680 kb on - strand, within Rru_A2503 at 2909.680 kb on - strand, within Rru_A2503 at 2909.763 kb on + strand, within Rru_A2503 at 2909.764 kb on - strand, within Rru_A2503 at 2909.764 kb on - strand, within Rru_A2503 at 2909.926 kb on + strand, within Rru_A2503 at 2909.926 kb on + strand, within Rru_A2503 at 2909.927 kb on - strand, within Rru_A2503 at 2909.927 kb on - strand, within Rru_A2503 at 2909.927 kb on - strand, within Rru_A2503 at 2910.078 kb on + strand at 2910.101 kb on + strand at 2910.101 kb on + strand at 2910.103 kb on + strand at 2910.103 kb on + strand at 2910.103 kb on + strand at 2910.103 kb on + strand at 2910.148 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 2,908,426 - Rru_A2501 0.55 +0.0 2,908,546 + Rru_A2501 0.78 -2.0 2,908,547 - Rru_A2501 0.78 +0.0 2,908,748 + +0.0 2,908,748 + +0.0 2,908,748 + +0.0 2,908,749 - +0.0 2,908,749 - +0.0 2,908,749 - +0.0 2,908,749 - -3.1 2,908,752 + +0.0 2,908,753 - +0.0 2,908,930 + Rru_A2502 0.27 +0.0 2,908,931 - Rru_A2502 0.27 +0.0 2,908,931 - Rru_A2502 0.27 -1.6 2,909,097 + Rru_A2502 0.63 -3.0 2,909,098 - Rru_A2502 0.63 +0.0 2,909,098 - Rru_A2502 0.63 -0.9 2,909,319 + +0.0 2,909,320 - -2.6 2,909,320 - -2.1 2,909,320 - -2.2 2,909,373 + Rru_A2503 0.16 -1.0 2,909,373 + Rru_A2503 0.16 -2.5 2,909,373 + Rru_A2503 0.16 -2.5 2,909,373 + Rru_A2503 0.16 -3.7 2,909,373 + Rru_A2503 0.16 -2.6 2,909,374 - Rru_A2503 0.16 +0.0 2,909,505 + Rru_A2503 0.33 -2.4 2,909,505 + Rru_A2503 0.33 +0.0 2,909,506 - Rru_A2503 0.33 -3.6 2,909,506 - Rru_A2503 0.33 -4.7 2,909,514 + Rru_A2503 0.34 +0.0 2,909,515 - Rru_A2503 0.34 -2.3 2,909,586 + Rru_A2503 0.43 -1.5 2,909,606 - Rru_A2503 0.45 +0.0 2,909,661 + Rru_A2503 0.52 -2.8 2,909,661 + Rru_A2503 0.52 -2.9 2,909,662 - Rru_A2503 0.52 +0.0 2,909,662 - Rru_A2503 0.52 -3.4 2,909,679 + Rru_A2503 0.54 -2.2 2,909,680 - Rru_A2503 0.55 -1.9 2,909,680 - Rru_A2503 0.55 +0.0 2,909,763 + Rru_A2503 0.65 +0.0 2,909,764 - Rru_A2503 0.65 -2.8 2,909,764 - Rru_A2503 0.65 -2.1 2,909,926 + Rru_A2503 0.86 -2.7 2,909,926 + Rru_A2503 0.86 +0.0 2,909,927 - Rru_A2503 0.86 +0.0 2,909,927 - Rru_A2503 0.86 +0.0 2,909,927 - Rru_A2503 0.86 +0.0 2,910,078 + -0.9 2,910,101 + +0.0 2,910,101 + +0.0 2,910,103 + +0.0 2,910,103 + +0.0 2,910,103 + +0.0 2,910,103 + +0.0 2,910,148 + +0.0
Or see this region's nucleotide sequence