Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2349 and Rru_A2350 overlap by 40 nucleotides Rru_A2350 and Rru_A2351 are separated by 33 nucleotides Rru_A2351 and Rru_AR0041 are separated by 449 nucleotides Rru_AR0041 and Rru_AR0042 are separated by 95 nucleotides Rru_AR0042 and Rru_AR0043 are separated by 119 nucleotides
Rru_A2349: Rru_A2349 - hypothetical protein (NCBI), at 2,727,775 to 2,728,638
_A2349
Rru_A2350: Rru_A2350 - Methylase involved in ubiquinone/menaquinone biosynthesis-like (NCBI), at 2,728,599 to 2,729,564
_A2350
Rru_A2351: Rru_A2351 - Macrocin-O-methyltransferase (NCBI), at 2,729,598 to 2,731,271
_A2351
Rru_AR0041: Rru_AR0041 - tRNA-Met (NCBI), at 2,731,721 to 2,731,797
_AR0041
Rru_AR0042: Rru_AR0042 - 5S ribosomal RNA (NCBI), at 2,731,893 to 2,732,007
_AR0042
Rru_AR0043: Rru_AR0043 - 23S ribosomal RNA (NCBI), at 2,732,127 to 2,734,884
_AR0043
Position (kb)
2729
2730
2731
2732 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2728.697 kb on - strand, within Rru_A2350 at 2728.697 kb on - strand, within Rru_A2350 at 2728.765 kb on + strand, within Rru_A2350 at 2728.765 kb on + strand, within Rru_A2350 at 2728.765 kb on + strand, within Rru_A2350 at 2728.765 kb on + strand, within Rru_A2350 at 2728.832 kb on + strand, within Rru_A2350 at 2728.990 kb on + strand, within Rru_A2350 at 2728.990 kb on + strand, within Rru_A2350 at 2728.990 kb on + strand, within Rru_A2350 at 2728.991 kb on - strand, within Rru_A2350 at 2728.991 kb on - strand, within Rru_A2350 at 2729.014 kb on + strand, within Rru_A2350 at 2729.014 kb on + strand, within Rru_A2350 at 2729.014 kb on + strand, within Rru_A2350 at 2729.014 kb on + strand, within Rru_A2350 at 2729.014 kb on + strand, within Rru_A2350 at 2729.015 kb on - strand, within Rru_A2350 at 2729.015 kb on - strand, within Rru_A2350 at 2729.181 kb on + strand, within Rru_A2350 at 2729.181 kb on + strand, within Rru_A2350 at 2729.181 kb on + strand, within Rru_A2350 at 2729.182 kb on - strand, within Rru_A2350 at 2729.182 kb on - strand, within Rru_A2350 at 2729.409 kb on + strand, within Rru_A2350 at 2729.607 kb on + strand at 2729.608 kb on - strand at 2729.647 kb on - strand at 2729.685 kb on + strand at 2729.685 kb on + strand at 2729.686 kb on - strand at 2729.712 kb on + strand at 2729.712 kb on + strand at 2729.758 kb on + strand at 2729.759 kb on - strand at 2729.863 kb on - strand, within Rru_A2351 at 2729.876 kb on + strand, within Rru_A2351 at 2729.876 kb on + strand, within Rru_A2351 at 2729.876 kb on + strand, within Rru_A2351 at 2729.876 kb on + strand, within Rru_A2351 at 2730.117 kb on + strand, within Rru_A2351 at 2730.190 kb on - strand, within Rru_A2351 at 2730.214 kb on + strand, within Rru_A2351 at 2730.214 kb on + strand, within Rru_A2351 at 2730.214 kb on + strand, within Rru_A2351 at 2730.215 kb on - strand, within Rru_A2351 at 2730.297 kb on + strand, within Rru_A2351 at 2730.331 kb on - strand, within Rru_A2351 at 2730.573 kb on + strand, within Rru_A2351 at 2730.573 kb on + strand, within Rru_A2351 at 2730.574 kb on - strand, within Rru_A2351 at 2730.574 kb on - strand, within Rru_A2351 at 2730.642 kb on + strand, within Rru_A2351 at 2730.777 kb on - strand, within Rru_A2351 at 2730.861 kb on + strand, within Rru_A2351 at 2730.861 kb on + strand, within Rru_A2351 at 2730.945 kb on - strand, within Rru_A2351 at 2731.047 kb on + strand, within Rru_A2351 at 2731.047 kb on + strand, within Rru_A2351 at 2731.047 kb on + strand, within Rru_A2351 at 2731.513 kb on + strand at 2731.514 kb on - strand at 2731.544 kb on - strand at 2731.551 kb on + strand at 2731.551 kb on + strand at 2731.552 kb on - strand at 2731.799 kb on + strand at 2731.908 kb on - strand, within Rru_AR0042 at 2731.908 kb on - strand, within Rru_AR0042 at 2731.908 kb on - strand, within Rru_AR0042 at 2731.908 kb on - strand, within Rru_AR0042
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 2,728,697 - Rru_A2350 0.10 +0.0 2,728,697 - Rru_A2350 0.10 +0.0 2,728,765 + Rru_A2350 0.17 +0.0 2,728,765 + Rru_A2350 0.17 +0.0 2,728,765 + Rru_A2350 0.17 +0.0 2,728,765 + Rru_A2350 0.17 +0.0 2,728,832 + Rru_A2350 0.24 +0.0 2,728,990 + Rru_A2350 0.40 +0.0 2,728,990 + Rru_A2350 0.40 +0.0 2,728,990 + Rru_A2350 0.40 -2.4 2,728,991 - Rru_A2350 0.41 -2.3 2,728,991 - Rru_A2350 0.41 +0.0 2,729,014 + Rru_A2350 0.43 +0.0 2,729,014 + Rru_A2350 0.43 -3.0 2,729,014 + Rru_A2350 0.43 -1.8 2,729,014 + Rru_A2350 0.43 +0.0 2,729,014 + Rru_A2350 0.43 +0.0 2,729,015 - Rru_A2350 0.43 -3.1 2,729,015 - Rru_A2350 0.43 -2.8 2,729,181 + Rru_A2350 0.60 +0.0 2,729,181 + Rru_A2350 0.60 -2.4 2,729,181 + Rru_A2350 0.60 +0.0 2,729,182 - Rru_A2350 0.60 +0.0 2,729,182 - Rru_A2350 0.60 +0.0 2,729,409 + Rru_A2350 0.84 +0.0 2,729,607 + -1.9 2,729,608 - -3.5 2,729,647 - +0.0 2,729,685 + +0.0 2,729,685 + +0.0 2,729,686 - +0.0 2,729,712 + +0.0 2,729,712 + +0.0 2,729,758 + +0.0 2,729,759 - +0.0 2,729,863 - Rru_A2351 0.16 +0.0 2,729,876 + Rru_A2351 0.17 +0.0 2,729,876 + Rru_A2351 0.17 +0.0 2,729,876 + Rru_A2351 0.17 -3.1 2,729,876 + Rru_A2351 0.17 -1.6 2,730,117 + Rru_A2351 0.31 +0.0 2,730,190 - Rru_A2351 0.35 -1.0 2,730,214 + Rru_A2351 0.37 +0.0 2,730,214 + Rru_A2351 0.37 +0.0 2,730,214 + Rru_A2351 0.37 +0.0 2,730,215 - Rru_A2351 0.37 +0.0 2,730,297 + Rru_A2351 0.42 -3.8 2,730,331 - Rru_A2351 0.44 -2.7 2,730,573 + Rru_A2351 0.58 +0.0 2,730,573 + Rru_A2351 0.58 -3.6 2,730,574 - Rru_A2351 0.58 +0.0 2,730,574 - Rru_A2351 0.58 -3.3 2,730,642 + Rru_A2351 0.62 +0.0 2,730,777 - Rru_A2351 0.70 +0.0 2,730,861 + Rru_A2351 0.75 +0.0 2,730,861 + Rru_A2351 0.75 +0.0 2,730,945 - Rru_A2351 0.80 -3.2 2,731,047 + Rru_A2351 0.87 +0.0 2,731,047 + Rru_A2351 0.87 -1.0 2,731,047 + Rru_A2351 0.87 -1.6 2,731,513 + +0.0 2,731,514 - +0.0 2,731,544 - +0.0 2,731,551 + +0.0 2,731,551 + -2.2 2,731,552 - -3.3 2,731,799 + +0.0 2,731,908 - Rru_AR0042 0.13 +0.0 2,731,908 - Rru_AR0042 0.13 -1.9 2,731,908 - Rru_AR0042 0.13 -1.6 2,731,908 - Rru_AR0042 0.13 -2.1
Or see this region's nucleotide sequence