Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2197 and Rru_A2198 are separated by 161 nucleotides Rru_A2198 and Rru_A2199 are separated by 96 nucleotides Rru_A2199 and Rru_A2200 are separated by 131 nucleotides Rru_A2200 and Rru_A2201 are separated by 101 nucleotides
Rru_A2197: Rru_A2197 - 3,4-Dihydroxy-2-butanone 4-phosphate synthase (NCBI), at 2,551,477 to 2,552,121
_A2197
Rru_A2198: Rru_A2198 - Protein of unknown function UPF0066 (NCBI), at 2,552,283 to 2,552,774
_A2198
Rru_A2199: Rru_A2199 - hypothetical protein (NCBI), at 2,552,871 to 2,554,577
_A2199
Rru_A2200: Rru_A2200 - DNA polymerase III chi subunit, HolC (NCBI), at 2,554,709 to 2,555,191
_A2200
Rru_A2201: Rru_A2201 - Peptidase S1C, Do (NCBI), at 2,555,293 to 2,556,819
_A2201
Position (kb)
2552
2553
2554
2555 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2552.200 kb on + strand at 2552.222 kb on - strand at 2552.232 kb on + strand at 2552.233 kb on - strand at 2552.233 kb on - strand at 2552.285 kb on - strand at 2552.323 kb on + strand at 2552.323 kb on + strand at 2552.323 kb on + strand at 2552.323 kb on + strand at 2552.324 kb on - strand at 2552.324 kb on - strand at 2552.324 kb on - strand at 2552.324 kb on - strand at 2552.324 kb on - strand at 2552.324 kb on - strand at 2552.324 kb on - strand at 2552.326 kb on + strand at 2552.326 kb on + strand at 2552.327 kb on - strand at 2552.539 kb on + strand, within Rru_A2198 at 2552.539 kb on + strand, within Rru_A2198 at 2552.539 kb on + strand, within Rru_A2198 at 2552.540 kb on - strand, within Rru_A2198 at 2552.699 kb on + strand, within Rru_A2198 at 2552.820 kb on - strand at 2552.906 kb on - strand at 2553.373 kb on + strand, within Rru_A2199 at 2553.373 kb on + strand, within Rru_A2199 at 2553.374 kb on - strand, within Rru_A2199 at 2554.090 kb on + strand, within Rru_A2199 at 2554.090 kb on + strand, within Rru_A2199 at 2554.090 kb on + strand, within Rru_A2199 at 2554.090 kb on + strand, within Rru_A2199 at 2554.091 kb on - strand, within Rru_A2199 at 2554.091 kb on - strand, within Rru_A2199 at 2554.091 kb on - strand, within Rru_A2199 at 2554.091 kb on - strand, within Rru_A2199 at 2554.091 kb on - strand, within Rru_A2199 at 2554.261 kb on + strand, within Rru_A2199 at 2554.262 kb on - strand, within Rru_A2199 at 2554.262 kb on - strand, within Rru_A2199 at 2554.306 kb on + strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.307 kb on - strand, within Rru_A2199 at 2554.477 kb on + strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.478 kb on - strand at 2554.519 kb on + strand at 2554.568 kb on - strand at 2554.568 kb on - strand at 2554.584 kb on - strand at 2554.615 kb on + strand at 2554.616 kb on - strand at 2554.629 kb on + strand at 2554.630 kb on - strand at 2554.665 kb on - strand at 2554.666 kb on + strand at 2554.666 kb on + strand at 2554.667 kb on - strand at 2554.667 kb on - strand at 2554.685 kb on + strand at 2554.685 kb on + strand at 2554.685 kb on + strand at 2554.710 kb on + strand at 2554.717 kb on + strand at 2554.718 kb on - strand at 2554.718 kb on - strand at 2554.785 kb on + strand, within Rru_A2200 at 2554.785 kb on + strand, within Rru_A2200 at 2554.785 kb on + strand, within Rru_A2200 at 2554.786 kb on - strand, within Rru_A2200 at 2555.165 kb on + strand at 2555.165 kb on + strand at 2555.172 kb on + strand at 2555.173 kb on - strand at 2555.202 kb on + strand at 2555.202 kb on + strand at 2555.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 2,552,200 + -2.0 2,552,222 - +0.0 2,552,232 + +0.0 2,552,233 - +0.0 2,552,233 - +0.0 2,552,285 - -2.6 2,552,323 + +0.0 2,552,323 + +0.0 2,552,323 + +0.0 2,552,323 + -2.4 2,552,324 - -2.4 2,552,324 - +0.0 2,552,324 - +0.0 2,552,324 - +0.0 2,552,324 - +0.0 2,552,324 - +0.0 2,552,324 - -2.2 2,552,326 + +0.0 2,552,326 + +0.0 2,552,327 - +0.0 2,552,539 + Rru_A2198 0.52 -0.4 2,552,539 + Rru_A2198 0.52 +0.0 2,552,539 + Rru_A2198 0.52 -2.9 2,552,540 - Rru_A2198 0.52 +0.0 2,552,699 + Rru_A2198 0.85 +0.0 2,552,820 - -2.5 2,552,906 - +0.0 2,553,373 + Rru_A2199 0.29 +0.0 2,553,373 + Rru_A2199 0.29 +0.0 2,553,374 - Rru_A2199 0.29 -2.3 2,554,090 + Rru_A2199 0.71 -3.3 2,554,090 + Rru_A2199 0.71 +0.0 2,554,090 + Rru_A2199 0.71 +0.0 2,554,090 + Rru_A2199 0.71 -3.8 2,554,091 - Rru_A2199 0.71 +0.0 2,554,091 - Rru_A2199 0.71 -5.7 2,554,091 - Rru_A2199 0.71 +0.0 2,554,091 - Rru_A2199 0.71 +0.0 2,554,091 - Rru_A2199 0.71 +0.0 2,554,261 + Rru_A2199 0.81 +0.0 2,554,262 - Rru_A2199 0.81 +0.0 2,554,262 - Rru_A2199 0.81 +0.0 2,554,306 + Rru_A2199 0.84 +0.0 2,554,307 - Rru_A2199 0.84 +0.0 2,554,307 - Rru_A2199 0.84 +0.0 2,554,307 - Rru_A2199 0.84 +0.0 2,554,307 - Rru_A2199 0.84 +0.0 2,554,307 - Rru_A2199 0.84 +0.0 2,554,307 - Rru_A2199 0.84 -3.5 2,554,307 - Rru_A2199 0.84 -3.4 2,554,477 + +0.0 2,554,478 - -2.5 2,554,478 - +0.0 2,554,478 - +0.0 2,554,478 - -2.5 2,554,478 - +0.0 2,554,478 - -2.6 2,554,478 - +0.0 2,554,478 - +0.0 2,554,478 - +0.0 2,554,478 - -2.3 2,554,519 + +0.0 2,554,568 - +0.0 2,554,568 - +0.0 2,554,584 - +0.0 2,554,615 + +0.0 2,554,616 - -1.8 2,554,629 + +0.0 2,554,630 - +0.0 2,554,665 - +0.0 2,554,666 + -2.3 2,554,666 + +0.0 2,554,667 - +0.0 2,554,667 - -1.0 2,554,685 + +0.0 2,554,685 + -2.7 2,554,685 + +0.0 2,554,710 + +0.0 2,554,717 + +0.0 2,554,718 - +0.0 2,554,718 - +0.0 2,554,785 + Rru_A2200 0.16 +0.0 2,554,785 + Rru_A2200 0.16 +0.0 2,554,785 + Rru_A2200 0.16 +0.0 2,554,786 - Rru_A2200 0.16 +0.0 2,555,165 + +0.0 2,555,165 + +0.0 2,555,172 + +0.0 2,555,173 - -3.4 2,555,202 + +0.0 2,555,202 + +0.0 2,555,295 - -3.6
Or see this region's nucleotide sequence