Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1013 and Rru_A1014 are separated by 34 nucleotides Rru_A1014 and Rru_A1015 are separated by 71 nucleotides Rru_A1015 and Rru_A1016 overlap by 1 nucleotides
Rru_A1013: Rru_A1013 - ABC transporter component (NCBI), at 1,201,234 to 1,203,066
_A1013
Rru_A1014: Rru_A1014 - hypothetical protein (NCBI), at 1,203,101 to 1,203,481
_A1014
Rru_A1015: Rru_A1015 - Binding-protein-dependent transport systems inner membrane component (NCBI), at 1,203,553 to 1,204,389
_A1015
Rru_A1016: Rru_A1016 - Binding-protein-dependent transport systems inner membrane component (NCBI), at 1,204,389 to 1,205,381
_A1016
Position (kb) 1203
1204 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1202.284 kb on + strand, within Rru_A1013 at 1202.285 kb on - strand, within Rru_A1013 at 1202.285 kb on - strand, within Rru_A1013 at 1202.285 kb on - strand, within Rru_A1013 at 1202.320 kb on - strand, within Rru_A1013 at 1202.398 kb on + strand, within Rru_A1013 at 1202.399 kb on - strand, within Rru_A1013 at 1202.400 kb on + strand, within Rru_A1013 at 1202.400 kb on + strand, within Rru_A1013 at 1202.401 kb on - strand, within Rru_A1013 at 1202.401 kb on - strand, within Rru_A1013 at 1202.401 kb on - strand, within Rru_A1013 at 1202.401 kb on - strand, within Rru_A1013 at 1202.587 kb on + strand, within Rru_A1013 at 1202.700 kb on + strand, within Rru_A1013 at 1202.840 kb on - strand, within Rru_A1013 at 1202.892 kb on + strand at 1202.960 kb on - strand at 1203.224 kb on + strand, within Rru_A1014 at 1203.224 kb on + strand, within Rru_A1014 at 1203.224 kb on + strand, within Rru_A1014 at 1203.224 kb on + strand, within Rru_A1014 at 1203.224 kb on + strand, within Rru_A1014 at 1203.225 kb on - strand, within Rru_A1014 at 1203.225 kb on - strand, within Rru_A1014 at 1203.480 kb on - strand at 1203.944 kb on + strand, within Rru_A1015 at 1203.944 kb on + strand, within Rru_A1015 at 1203.944 kb on + strand, within Rru_A1015 at 1203.945 kb on - strand, within Rru_A1015 at 1203.945 kb on - strand, within Rru_A1015 at 1203.945 kb on - strand, within Rru_A1015 at 1204.251 kb on - strand, within Rru_A1015 at 1204.251 kb on - strand, within Rru_A1015 at 1204.251 kb on - strand, within Rru_A1015 at 1204.307 kb on + strand at 1204.307 kb on + strand at 1204.307 kb on + strand at 1204.308 kb on - strand at 1204.308 kb on - strand at 1204.334 kb on + strand at 1204.334 kb on + strand at 1204.334 kb on + strand at 1204.334 kb on + strand at 1204.334 kb on + strand at 1204.335 kb on - strand at 1204.335 kb on - strand at 1204.390 kb on + strand at 1204.391 kb on - strand at 1204.391 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 1,202,284 + Rru_A1013 0.57 +0.0 1,202,285 - Rru_A1013 0.57 +0.0 1,202,285 - Rru_A1013 0.57 +0.0 1,202,285 - Rru_A1013 0.57 +0.0 1,202,320 - Rru_A1013 0.59 +0.0 1,202,398 + Rru_A1013 0.64 +0.0 1,202,399 - Rru_A1013 0.64 +0.0 1,202,400 + Rru_A1013 0.64 -3.1 1,202,400 + Rru_A1013 0.64 +0.0 1,202,401 - Rru_A1013 0.64 +0.0 1,202,401 - Rru_A1013 0.64 +0.0 1,202,401 - Rru_A1013 0.64 -0.7 1,202,401 - Rru_A1013 0.64 +0.0 1,202,587 + Rru_A1013 0.74 +0.0 1,202,700 + Rru_A1013 0.80 +0.0 1,202,840 - Rru_A1013 0.88 -2.7 1,202,892 + +0.0 1,202,960 - +0.0 1,203,224 + Rru_A1014 0.32 -1.0 1,203,224 + Rru_A1014 0.32 -3.3 1,203,224 + Rru_A1014 0.32 +0.0 1,203,224 + Rru_A1014 0.32 +0.0 1,203,224 + Rru_A1014 0.32 +0.0 1,203,225 - Rru_A1014 0.33 +0.0 1,203,225 - Rru_A1014 0.33 +0.0 1,203,480 - -2.1 1,203,944 + Rru_A1015 0.47 -3.9 1,203,944 + Rru_A1015 0.47 -1.0 1,203,944 + Rru_A1015 0.47 +0.0 1,203,945 - Rru_A1015 0.47 +0.0 1,203,945 - Rru_A1015 0.47 +0.0 1,203,945 - Rru_A1015 0.47 -1.0 1,204,251 - Rru_A1015 0.83 +0.0 1,204,251 - Rru_A1015 0.83 -1.6 1,204,251 - Rru_A1015 0.83 +0.0 1,204,307 + -3.1 1,204,307 + +0.0 1,204,307 + +0.0 1,204,308 - +0.0 1,204,308 - -2.3 1,204,334 + +0.0 1,204,334 + +0.0 1,204,334 + -3.2 1,204,334 + -3.5 1,204,334 + +0.0 1,204,335 - +0.0 1,204,335 - +0.0 1,204,390 + +0.0 1,204,391 - +0.0 1,204,391 - +0.0
Or see this region's nucleotide sequence