Experiment: L-Malic Acid (C) with ammonium chloride; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0211 and Rru_A0212 are separated by 93 nucleotides Rru_A0212 and Rru_A0213 are separated by 3 nucleotides Rru_A0213 and Rru_A0214 are separated by 402 nucleotides
Rru_A0211: Rru_A0211 - hypothetical protein (NCBI), at 263,151 to 263,912
_A0211
Rru_A0212: Rru_A0212 - hypothetical protein (NCBI), at 264,006 to 264,392
_A0212
Rru_A0213: Rru_A0213 - hypothetical protein (NCBI), at 264,396 to 265,736
_A0213
Rru_A0214: Rru_A0214 - MutT/nudix family protein (NCBI), at 266,139 to 267,017
_A0214
Position (kb) 264
265
266 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 263.431 kb on + strand, within Rru_A0211 at 263.432 kb on - strand, within Rru_A0211 at 263.432 kb on - strand, within Rru_A0211 at 263.702 kb on + strand, within Rru_A0211 at 263.702 kb on + strand, within Rru_A0211 at 263.702 kb on + strand, within Rru_A0211 at 263.703 kb on - strand, within Rru_A0211 at 263.703 kb on - strand, within Rru_A0211 at 263.973 kb on + strand at 263.980 kb on - strand at 264.007 kb on + strand at 264.007 kb on + strand at 264.008 kb on - strand at 264.008 kb on - strand at 264.184 kb on - strand, within Rru_A0212 at 264.397 kb on + strand at 264.397 kb on + strand at 265.155 kb on - strand, within Rru_A0213 at 265.258 kb on + strand, within Rru_A0213 at 265.259 kb on - strand, within Rru_A0213 at 265.259 kb on - strand, within Rru_A0213 at 265.807 kb on + strand at 265.808 kb on - strand at 265.816 kb on - strand at 265.826 kb on - strand at 265.849 kb on + strand at 265.849 kb on + strand at 266.158 kb on + strand at 266.158 kb on + strand at 266.158 kb on + strand at 266.487 kb on + strand, within Rru_A0214 at 266.488 kb on - strand, within Rru_A0214 at 266.488 kb on - strand, within Rru_A0214 at 266.562 kb on - strand, within Rru_A0214 at 266.591 kb on + strand, within Rru_A0214 at 266.591 kb on + strand, within Rru_A0214 at 266.591 kb on + strand, within Rru_A0214 at 266.715 kb on + strand, within Rru_A0214 at 266.715 kb on + strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214 at 266.716 kb on - strand, within Rru_A0214
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) with ammonium chloride; no light remove 263,431 + Rru_A0211 0.37 +0.0 263,432 - Rru_A0211 0.37 +0.0 263,432 - Rru_A0211 0.37 +0.0 263,702 + Rru_A0211 0.72 -2.9 263,702 + Rru_A0211 0.72 -1.0 263,702 + Rru_A0211 0.72 +0.0 263,703 - Rru_A0211 0.72 +0.0 263,703 - Rru_A0211 0.72 +0.0 263,973 + +0.0 263,980 - -2.2 264,007 + +0.0 264,007 + +0.0 264,008 - -3.1 264,008 - -2.5 264,184 - Rru_A0212 0.46 +0.0 264,397 + +0.0 264,397 + -1.1 265,155 - Rru_A0213 0.57 +0.0 265,258 + Rru_A0213 0.64 -2.5 265,259 - Rru_A0213 0.64 -3.6 265,259 - Rru_A0213 0.64 +0.0 265,807 + -3.8 265,808 - +0.0 265,816 - -1.3 265,826 - -2.3 265,849 + +0.0 265,849 + +0.0 266,158 + +0.0 266,158 + +0.0 266,158 + +0.0 266,487 + Rru_A0214 0.40 +0.0 266,488 - Rru_A0214 0.40 +0.0 266,488 - Rru_A0214 0.40 +0.0 266,562 - Rru_A0214 0.48 +0.0 266,591 + Rru_A0214 0.51 +0.0 266,591 + Rru_A0214 0.51 +0.0 266,591 + Rru_A0214 0.51 +0.0 266,715 + Rru_A0214 0.66 +0.0 266,715 + Rru_A0214 0.66 +0.0 266,716 - Rru_A0214 0.66 -2.3 266,716 - Rru_A0214 0.66 +0.0 266,716 - Rru_A0214 0.66 +0.0 266,716 - Rru_A0214 0.66 +0.0 266,716 - Rru_A0214 0.66 -2.4 266,716 - Rru_A0214 0.66 +0.0 266,716 - Rru_A0214 0.66 +0.0
Or see this region's nucleotide sequence