Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_572

Experiment: Nickel (II) chloride 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_571 and Pf6N2E2_572 are separated by 184 nucleotidesPf6N2E2_572 and Pf6N2E2_573 are separated by 67 nucleotides Pf6N2E2_571 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 645,326 to 646,369 _571 Pf6N2E2_572 - hypothetical protein, at 646,554 to 647,033 _572 Pf6N2E2_573 - Potassium uptake protein TrkH, at 647,101 to 648,555 _573 Position (kb) 646 647 648Strain fitness (log2 ratio) -2 -1 0 1 2at 645.721 kb on - strand, within Pf6N2E2_571at 645.725 kb on + strand, within Pf6N2E2_571at 645.726 kb on - strand, within Pf6N2E2_571at 645.726 kb on - strand, within Pf6N2E2_571at 645.726 kb on - strand, within Pf6N2E2_571at 645.726 kb on - strand, within Pf6N2E2_571at 645.726 kb on - strand, within Pf6N2E2_571at 646.015 kb on - strand, within Pf6N2E2_571at 646.015 kb on - strand, within Pf6N2E2_571at 646.015 kb on - strand, within Pf6N2E2_571at 646.298 kb on - strandat 646.400 kb on + strandat 646.400 kb on + strandat 646.420 kb on + strandat 646.420 kb on + strandat 646.420 kb on + strandat 646.420 kb on + strandat 646.421 kb on - strandat 646.421 kb on - strandat 646.517 kb on - strandat 646.565 kb on + strandat 646.565 kb on + strandat 646.566 kb on - strandat 646.656 kb on + strand, within Pf6N2E2_572at 646.656 kb on + strand, within Pf6N2E2_572at 646.926 kb on - strand, within Pf6N2E2_572at 646.973 kb on + strand, within Pf6N2E2_572at 647.106 kb on - strandat 647.174 kb on - strandat 647.347 kb on - strand, within Pf6N2E2_573at 647.347 kb on - strand, within Pf6N2E2_573at 647.412 kb on - strand, within Pf6N2E2_573at 647.467 kb on + strand, within Pf6N2E2_573at 647.468 kb on - strand, within Pf6N2E2_573at 647.480 kb on - strand, within Pf6N2E2_573at 647.480 kb on - strand, within Pf6N2E2_573at 647.546 kb on + strand, within Pf6N2E2_573at 647.547 kb on - strand, within Pf6N2E2_573at 647.547 kb on - strand, within Pf6N2E2_573at 647.547 kb on - strand, within Pf6N2E2_573at 647.579 kb on - strand, within Pf6N2E2_573at 647.585 kb on + strand, within Pf6N2E2_573at 647.585 kb on + strand, within Pf6N2E2_573at 647.585 kb on + strand, within Pf6N2E2_573at 647.586 kb on - strand, within Pf6N2E2_573at 647.586 kb on - strand, within Pf6N2E2_573at 647.790 kb on - strand, within Pf6N2E2_573at 647.990 kb on + strand, within Pf6N2E2_573at 647.990 kb on + strand, within Pf6N2E2_573at 647.990 kb on + strand, within Pf6N2E2_573at 647.990 kb on + strand, within Pf6N2E2_573at 647.991 kb on - strand, within Pf6N2E2_573at 647.991 kb on - strand, within Pf6N2E2_573at 648.005 kb on + strand, within Pf6N2E2_573at 648.005 kb on + strand, within Pf6N2E2_573at 648.005 kb on + strand, within Pf6N2E2_573at 648.005 kb on + strand, within Pf6N2E2_573at 648.006 kb on - strand, within Pf6N2E2_573at 648.006 kb on - strand, within Pf6N2E2_573at 648.006 kb on - strand, within Pf6N2E2_573at 648.006 kb on - strand, within Pf6N2E2_573at 648.006 kb on - strand, within Pf6N2E2_573at 648.006 kb on - strand, within Pf6N2E2_573

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M
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645,721 - Pf6N2E2_571 0.38 +0.3
645,725 + Pf6N2E2_571 0.38 +0.8
645,726 - Pf6N2E2_571 0.38 -0.2
645,726 - Pf6N2E2_571 0.38 +0.2
645,726 - Pf6N2E2_571 0.38 +0.4
645,726 - Pf6N2E2_571 0.38 -0.4
645,726 - Pf6N2E2_571 0.38 -0.4
646,015 - Pf6N2E2_571 0.66 +0.4
646,015 - Pf6N2E2_571 0.66 +0.3
646,015 - Pf6N2E2_571 0.66 -1.3
646,298 - -0.9
646,400 + +0.0
646,400 + +0.3
646,420 + -0.1
646,420 + +0.4
646,420 + -0.7
646,420 + +1.4
646,421 - -0.8
646,421 - +0.1
646,517 - -1.8
646,565 + -0.4
646,565 + -2.2
646,566 - +0.0
646,656 + Pf6N2E2_572 0.21 +1.9
646,656 + Pf6N2E2_572 0.21 -0.6
646,926 - Pf6N2E2_572 0.78 -0.7
646,973 + Pf6N2E2_572 0.87 -2.0
647,106 - +0.0
647,174 - -0.1
647,347 - Pf6N2E2_573 0.17 +0.7
647,347 - Pf6N2E2_573 0.17 +0.3
647,412 - Pf6N2E2_573 0.21 -1.4
647,467 + Pf6N2E2_573 0.25 +1.5
647,468 - Pf6N2E2_573 0.25 +0.3
647,480 - Pf6N2E2_573 0.26 +1.0
647,480 - Pf6N2E2_573 0.26 +0.2
647,546 + Pf6N2E2_573 0.31 -1.4
647,547 - Pf6N2E2_573 0.31 -0.8
647,547 - Pf6N2E2_573 0.31 -0.1
647,547 - Pf6N2E2_573 0.31 -0.2
647,579 - Pf6N2E2_573 0.33 +0.5
647,585 + Pf6N2E2_573 0.33 +0.8
647,585 + Pf6N2E2_573 0.33 -1.4
647,585 + Pf6N2E2_573 0.33 -0.8
647,586 - Pf6N2E2_573 0.33 +0.2
647,586 - Pf6N2E2_573 0.33 -1.8
647,790 - Pf6N2E2_573 0.47 +1.5
647,990 + Pf6N2E2_573 0.61 +1.2
647,990 + Pf6N2E2_573 0.61 -0.1
647,990 + Pf6N2E2_573 0.61 +1.2
647,990 + Pf6N2E2_573 0.61 +0.3
647,991 - Pf6N2E2_573 0.61 -0.6
647,991 - Pf6N2E2_573 0.61 -1.2
648,005 + Pf6N2E2_573 0.62 -1.2
648,005 + Pf6N2E2_573 0.62 +0.4
648,005 + Pf6N2E2_573 0.62 -1.0
648,005 + Pf6N2E2_573 0.62 -0.1
648,006 - Pf6N2E2_573 0.62 +0.9
648,006 - Pf6N2E2_573 0.62 +1.2
648,006 - Pf6N2E2_573 0.62 +0.5
648,006 - Pf6N2E2_573 0.62 -1.7
648,006 - Pf6N2E2_573 0.62 -0.4
648,006 - Pf6N2E2_573 0.62 -1.5

Or see this region's nucleotide sequence