Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_428

Experiment: Nickel (II) chloride 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_425 and Pf6N2E2_426 are separated by 4 nucleotidesPf6N2E2_426 and Pf6N2E2_427 are separated by 161 nucleotidesPf6N2E2_427 and Pf6N2E2_428 are separated by 125 nucleotidesPf6N2E2_428 and Pf6N2E2_429 are separated by 117 nucleotides Pf6N2E2_425 - FIG00957304: hypothetical protein, at 494,309 to 494,842 _425 Pf6N2E2_426 - Arsenate reductase (EC 1.20.4.1), at 494,847 to 495,302 _426 Pf6N2E2_427 - Conidiation-specific protein 10, at 495,464 to 495,643 _427 Pf6N2E2_428 - Protease subunit of ATP-dependent Clp proteases, at 495,769 to 496,332 _428 Pf6N2E2_429 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 496,450 to 497,505 _429 Position (kb) 495 496 497Strain fitness (log2 ratio) -2 -1 0 1at 494.828 kb on + strandat 495.046 kb on + strand, within Pf6N2E2_426at 495.047 kb on - strand, within Pf6N2E2_426at 495.047 kb on - strand, within Pf6N2E2_426at 495.047 kb on - strand, within Pf6N2E2_426at 495.147 kb on + strand, within Pf6N2E2_426at 495.147 kb on + strand, within Pf6N2E2_426at 495.148 kb on - strand, within Pf6N2E2_426at 495.148 kb on - strand, within Pf6N2E2_426at 495.148 kb on - strand, within Pf6N2E2_426at 495.148 kb on - strand, within Pf6N2E2_426at 495.358 kb on + strandat 495.358 kb on + strandat 495.359 kb on - strandat 495.359 kb on - strandat 495.359 kb on - strandat 495.360 kb on + strandat 495.377 kb on - strandat 495.378 kb on + strandat 495.689 kb on - strandat 495.689 kb on - strandat 495.770 kb on + strandat 495.771 kb on - strandat 495.771 kb on - strandat 495.818 kb on - strandat 495.906 kb on + strand, within Pf6N2E2_428at 495.944 kb on + strand, within Pf6N2E2_428at 495.944 kb on + strand, within Pf6N2E2_428at 495.944 kb on + strand, within Pf6N2E2_428at 495.990 kb on - strand, within Pf6N2E2_428at 495.990 kb on - strand, within Pf6N2E2_428at 496.031 kb on + strand, within Pf6N2E2_428at 496.032 kb on - strand, within Pf6N2E2_428at 496.097 kb on + strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.098 kb on - strand, within Pf6N2E2_428at 496.144 kb on + strand, within Pf6N2E2_428at 496.145 kb on - strand, within Pf6N2E2_428at 496.145 kb on - strand, within Pf6N2E2_428at 496.175 kb on + strand, within Pf6N2E2_428at 496.175 kb on + strand, within Pf6N2E2_428at 496.266 kb on + strand, within Pf6N2E2_428at 496.387 kb on + strandat 496.388 kb on - strandat 496.508 kb on - strandat 496.542 kb on + strandat 496.542 kb on + strandat 496.543 kb on - strandat 496.543 kb on - strandat 496.613 kb on + strand, within Pf6N2E2_429at 496.613 kb on + strand, within Pf6N2E2_429at 496.614 kb on - strand, within Pf6N2E2_429at 496.614 kb on - strand, within Pf6N2E2_429at 497.027 kb on + strand, within Pf6N2E2_429at 497.027 kb on + strand, within Pf6N2E2_429at 497.028 kb on - strand, within Pf6N2E2_429at 497.028 kb on - strand, within Pf6N2E2_429at 497.201 kb on + strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.202 kb on - strand, within Pf6N2E2_429at 497.247 kb on - strand, within Pf6N2E2_429at 497.322 kb on + strand, within Pf6N2E2_429at 497.324 kb on + strand, within Pf6N2E2_429at 497.324 kb on + strand, within Pf6N2E2_429at 497.324 kb on + strand, within Pf6N2E2_429

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M
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494,828 + -1.3
495,046 + Pf6N2E2_426 0.44 -1.4
495,047 - Pf6N2E2_426 0.44 +0.2
495,047 - Pf6N2E2_426 0.44 +0.0
495,047 - Pf6N2E2_426 0.44 -0.7
495,147 + Pf6N2E2_426 0.66 +1.4
495,147 + Pf6N2E2_426 0.66 +1.1
495,148 - Pf6N2E2_426 0.66 +1.1
495,148 - Pf6N2E2_426 0.66 +1.0
495,148 - Pf6N2E2_426 0.66 -0.7
495,148 - Pf6N2E2_426 0.66 -0.2
495,358 + +0.3
495,358 + -0.2
495,359 - -0.3
495,359 - -2.1
495,359 - -0.9
495,360 + -0.2
495,377 - -1.2
495,378 + -0.0
495,689 - +0.2
495,689 - -0.0
495,770 + +0.5
495,771 - -1.7
495,771 - -1.4
495,818 - +1.5
495,906 + Pf6N2E2_428 0.24 -0.4
495,944 + Pf6N2E2_428 0.31 -1.0
495,944 + Pf6N2E2_428 0.31 -0.9
495,944 + Pf6N2E2_428 0.31 +0.4
495,990 - Pf6N2E2_428 0.39 +0.4
495,990 - Pf6N2E2_428 0.39 -0.2
496,031 + Pf6N2E2_428 0.46 -0.3
496,032 - Pf6N2E2_428 0.47 +0.2
496,097 + Pf6N2E2_428 0.58 -0.2
496,098 - Pf6N2E2_428 0.58 +0.4
496,098 - Pf6N2E2_428 0.58 -0.6
496,098 - Pf6N2E2_428 0.58 -1.1
496,098 - Pf6N2E2_428 0.58 -1.2
496,098 - Pf6N2E2_428 0.58 +0.6
496,098 - Pf6N2E2_428 0.58 +0.2
496,144 + Pf6N2E2_428 0.66 +0.2
496,145 - Pf6N2E2_428 0.67 -0.4
496,145 - Pf6N2E2_428 0.67 -0.6
496,175 + Pf6N2E2_428 0.72 +1.0
496,175 + Pf6N2E2_428 0.72 -1.0
496,266 + Pf6N2E2_428 0.88 -1.6
496,387 + +0.4
496,388 - +0.1
496,508 - +0.4
496,542 + -1.3
496,542 + +1.6
496,543 - -0.5
496,543 - +1.5
496,613 + Pf6N2E2_429 0.15 +0.9
496,613 + Pf6N2E2_429 0.15 -0.6
496,614 - Pf6N2E2_429 0.16 -0.3
496,614 - Pf6N2E2_429 0.16 +0.1
497,027 + Pf6N2E2_429 0.55 -0.5
497,027 + Pf6N2E2_429 0.55 -0.8
497,028 - Pf6N2E2_429 0.55 +0.0
497,028 - Pf6N2E2_429 0.55 -0.2
497,201 + Pf6N2E2_429 0.71 -1.6
497,202 - Pf6N2E2_429 0.71 +0.2
497,202 - Pf6N2E2_429 0.71 +0.6
497,202 - Pf6N2E2_429 0.71 -0.0
497,202 - Pf6N2E2_429 0.71 -0.4
497,202 - Pf6N2E2_429 0.71 +0.6
497,247 - Pf6N2E2_429 0.75 +0.5
497,322 + Pf6N2E2_429 0.83 +0.2
497,324 + Pf6N2E2_429 0.83 +0.6
497,324 + Pf6N2E2_429 0.83 +0.5
497,324 + Pf6N2E2_429 0.83 -1.3

Or see this region's nucleotide sequence