Experiment: Nickel (II) chloride 0.0025 M 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_3531 and Pf6N2E2_3532 overlap by 1 nucleotides Pf6N2E2_3532 and Pf6N2E2_3533 overlap by 4 nucleotides Pf6N2E2_3533 and Pf6N2E2_3534 overlap by 8 nucleotides 
        Pf6N2E2_3531 - Cobalamin biosynthesis protein CbiG / Cobalt-precorrin-3b C17-methyltransferase, at 4,121,025 to 4,122,713 
        _3531 
         
        
        Pf6N2E2_3532 - Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130), at 4,122,713 to 4,123,447 
        _3532 
         
        
        Pf6N2E2_3533 - Cobalt-precorrin-8x methylmutase (EC 5.4.1.2), at 4,123,444 to 4,124,070 
        _3533 
         
        
        Pf6N2E2_3534 - Cobalamin biosynthesis protein CobG, at 4,124,063 to 4,125,331 
        _3534 
         Position (kb) 4123 
4124 
4125 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 4122.551 kb on - strand at 4122.583 kb on + strand at 4122.583 kb on + strand at 4122.583 kb on + strand at 4122.584 kb on - strand at 4122.757 kb on - strand at 4122.828 kb on + strand, within Pf6N2E2_3532 at 4123.063 kb on - strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.089 kb on + strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.090 kb on - strand, within Pf6N2E2_3532 at 4123.095 kb on + strand, within Pf6N2E2_3532 at 4123.095 kb on + strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.096 kb on - strand, within Pf6N2E2_3532 at 4123.212 kb on + strand, within Pf6N2E2_3532 at 4123.213 kb on - strand, within Pf6N2E2_3532 at 4123.252 kb on - strand, within Pf6N2E2_3532 at 4123.339 kb on - strand, within Pf6N2E2_3532 at 4123.339 kb on - strand, within Pf6N2E2_3532 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.646 kb on + strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4123.647 kb on - strand, within Pf6N2E2_3533 at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.060 kb on + strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.061 kb on - strand at 4124.119 kb on + strand at 4124.119 kb on + strand at 4124.119 kb on + strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.120 kb on - strand at 4124.123 kb on + strand at 4124.123 kb on + strand at 4124.124 kb on - strand at 4124.124 kb on - strand at 4124.124 kb on - strand at 4124.175 kb on + strand at 4124.175 kb on + strand at 4124.494 kb on + strand, within Pf6N2E2_3534 at 4124.494 kb on + strand, within Pf6N2E2_3534 at 4124.495 kb on - strand, within Pf6N2E2_3534 at 4124.495 kb on - strand, within Pf6N2E2_3534 at 4125.035 kb on - strand, within Pf6N2E2_3534  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nickel (II) chloride 0.0025 M remove 4,122,551 -  -1.6 4,122,583 +  -0.4 4,122,583 +  +0.7 4,122,583 +  -0.4 4,122,584 -  +0.5 4,122,757 -  -1.0 4,122,828 +  Pf6N2E2_3532 0.16  -0.8 4,123,063 -  Pf6N2E2_3532 0.48  -0.2 4,123,089 +  Pf6N2E2_3532 0.51  -1.5 4,123,089 +  Pf6N2E2_3532 0.51  -0.3 4,123,089 +  Pf6N2E2_3532 0.51  -1.4 4,123,089 +  Pf6N2E2_3532 0.51  -0.7 4,123,089 +  Pf6N2E2_3532 0.51  +0.6 4,123,089 +  Pf6N2E2_3532 0.51  +1.9 4,123,089 +  Pf6N2E2_3532 0.51  -0.4 4,123,090 -  Pf6N2E2_3532 0.51  -0.6 4,123,090 -  Pf6N2E2_3532 0.51  +0.6 4,123,090 -  Pf6N2E2_3532 0.51  +0.2 4,123,095 +  Pf6N2E2_3532 0.52  +1.5 4,123,095 +  Pf6N2E2_3532 0.52  -0.9 4,123,096 -  Pf6N2E2_3532 0.52  -1.2 4,123,096 -  Pf6N2E2_3532 0.52  -0.1 4,123,096 -  Pf6N2E2_3532 0.52  +0.2 4,123,096 -  Pf6N2E2_3532 0.52  +0.7 4,123,096 -  Pf6N2E2_3532 0.52  -0.4 4,123,096 -  Pf6N2E2_3532 0.52  -0.1 4,123,096 -  Pf6N2E2_3532 0.52  -0.8 4,123,212 +  Pf6N2E2_3532 0.68  -1.2 4,123,213 -  Pf6N2E2_3532 0.68  -1.8 4,123,252 -  Pf6N2E2_3532 0.73  -0.0 4,123,339 -  Pf6N2E2_3532 0.85  +0.2 4,123,339 -  Pf6N2E2_3532 0.85  -1.3 4,123,646 +  Pf6N2E2_3533 0.32  -1.1 4,123,646 +  Pf6N2E2_3533 0.32  -0.7 4,123,646 +  Pf6N2E2_3533 0.32  -3.0 4,123,647 -  Pf6N2E2_3533 0.32  +0.0 4,123,647 -  Pf6N2E2_3533 0.32  -1.2 4,123,647 -  Pf6N2E2_3533 0.32  -0.7 4,123,647 -  Pf6N2E2_3533 0.32  -0.3 4,123,647 -  Pf6N2E2_3533 0.32  +0.4 4,124,060 +  +1.4 4,124,060 +  +1.6 4,124,060 +  -2.4 4,124,060 +  +0.9 4,124,060 +  -0.4 4,124,060 +  +0.2 4,124,060 +  +0.3 4,124,061 -  -2.0 4,124,061 -  -1.0 4,124,061 -  -0.6 4,124,061 -  -1.2 4,124,119 +  -1.2 4,124,119 +  +1.4 4,124,119 +  -1.7 4,124,120 -  -0.4 4,124,120 -  -0.2 4,124,120 -  -1.7 4,124,120 -  -1.8 4,124,120 -  -1.3 4,124,123 +  +0.1 4,124,123 +  -0.7 4,124,124 -  -1.7 4,124,124 -  +0.2 4,124,124 -  -1.0 4,124,175 +  -1.0 4,124,175 +  -0.1 4,124,494 +  Pf6N2E2_3534 0.34  -1.5 4,124,494 +  Pf6N2E2_3534 0.34  -0.8 4,124,495 -  Pf6N2E2_3534 0.34  -2.0 4,124,495 -  Pf6N2E2_3534 0.34  -1.8 4,125,035 -  Pf6N2E2_3534 0.77  -0.2 
Or see this region's nucleotide sequence