Experiment: Nickel (II) chloride 0.0025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3246 and Pf6N2E2_3247 are separated by 45 nucleotides Pf6N2E2_3247 and Pf6N2E2_3248 are separated by 260 nucleotides Pf6N2E2_3248 and Pf6N2E2_3249 are separated by 140 nucleotides
Pf6N2E2_3246 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 3,755,063 to 3,756,028
_3246
Pf6N2E2_3247 - Ribosomal RNA small subunit methyltransferase C (EC 2.1.1.52), at 3,756,074 to 3,757,072
_3247
Pf6N2E2_3248 - Probable transmembrane protein, at 3,757,333 to 3,757,917
_3248
Pf6N2E2_3249 - Zn-dependent protease with chaperone function, at 3,758,058 to 3,758,873
_3249
Position (kb)
3756
3757
3758 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3756.005 kb on - strand at 3756.029 kb on + strand at 3756.030 kb on - strand at 3756.058 kb on + strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.059 kb on - strand at 3756.094 kb on - strand at 3756.094 kb on - strand at 3756.094 kb on - strand at 3756.115 kb on - strand at 3756.323 kb on + strand, within Pf6N2E2_3247 at 3756.323 kb on + strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.324 kb on - strand, within Pf6N2E2_3247 at 3756.368 kb on + strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.369 kb on - strand, within Pf6N2E2_3247 at 3756.531 kb on - strand, within Pf6N2E2_3247 at 3756.894 kb on - strand, within Pf6N2E2_3247 at 3757.052 kb on + strand at 3757.052 kb on + strand at 3757.080 kb on + strand at 3757.133 kb on + strand at 3757.141 kb on + strand at 3757.141 kb on + strand at 3757.141 kb on + strand at 3757.142 kb on - strand at 3757.142 kb on - strand at 3757.173 kb on + strand at 3757.173 kb on + strand at 3757.173 kb on + strand at 3757.173 kb on + strand at 3757.173 kb on + strand at 3757.174 kb on - strand at 3757.174 kb on - strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.183 kb on + strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.184 kb on - strand at 3757.207 kb on - strand at 3757.254 kb on + strand at 3757.254 kb on + strand at 3757.254 kb on + strand at 3757.254 kb on + strand at 3757.254 kb on + strand at 3757.254 kb on + strand at 3757.255 kb on - strand at 3757.255 kb on - strand at 3757.255 kb on - strand at 3757.290 kb on + strand at 3757.290 kb on + strand at 3757.290 kb on + strand at 3757.291 kb on - strand at 3757.291 kb on - strand at 3757.299 kb on + strand at 3757.300 kb on - strand at 3757.352 kb on + strand at 3757.353 kb on - strand at 3757.353 kb on - strand at 3757.353 kb on - strand at 3757.356 kb on + strand at 3757.357 kb on - strand at 3757.410 kb on + strand, within Pf6N2E2_3248 at 3757.410 kb on + strand, within Pf6N2E2_3248 at 3757.410 kb on + strand, within Pf6N2E2_3248 at 3757.410 kb on + strand, within Pf6N2E2_3248 at 3757.411 kb on - strand, within Pf6N2E2_3248 at 3757.411 kb on - strand, within Pf6N2E2_3248 at 3757.885 kb on + strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.886 kb on - strand at 3757.979 kb on - strand at 3758.043 kb on - strand at 3758.060 kb on - strand at 3758.060 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M remove 3,756,005 - +0.9 3,756,029 + -0.5 3,756,030 - -0.2 3,756,058 + -0.2 3,756,059 - +1.0 3,756,059 - +1.1 3,756,059 - -0.5 3,756,059 - +1.0 3,756,094 - -1.2 3,756,094 - -0.8 3,756,094 - -1.4 3,756,115 - -1.9 3,756,323 + Pf6N2E2_3247 0.25 -0.3 3,756,323 + Pf6N2E2_3247 0.25 -0.6 3,756,324 - Pf6N2E2_3247 0.25 -2.9 3,756,324 - Pf6N2E2_3247 0.25 -0.1 3,756,324 - Pf6N2E2_3247 0.25 -1.1 3,756,368 + Pf6N2E2_3247 0.29 +0.2 3,756,369 - Pf6N2E2_3247 0.30 -0.5 3,756,369 - Pf6N2E2_3247 0.30 -2.8 3,756,369 - Pf6N2E2_3247 0.30 +0.8 3,756,369 - Pf6N2E2_3247 0.30 -2.3 3,756,369 - Pf6N2E2_3247 0.30 -1.8 3,756,369 - Pf6N2E2_3247 0.30 -0.2 3,756,531 - Pf6N2E2_3247 0.46 +0.3 3,756,894 - Pf6N2E2_3247 0.82 -1.1 3,757,052 + -0.3 3,757,052 + -2.1 3,757,080 + -1.6 3,757,133 + +1.5 3,757,141 + -0.3 3,757,141 + +0.3 3,757,141 + -0.1 3,757,142 - +0.2 3,757,142 - +1.9 3,757,173 + +0.7 3,757,173 + +0.7 3,757,173 + +1.2 3,757,173 + -1.1 3,757,173 + +0.0 3,757,174 - +2.6 3,757,174 - +0.8 3,757,183 + -1.8 3,757,183 + -0.6 3,757,183 + -0.6 3,757,183 + +0.7 3,757,183 + -1.5 3,757,183 + +0.5 3,757,183 + +0.2 3,757,183 + -0.4 3,757,183 + +0.2 3,757,183 + -2.4 3,757,183 + +0.4 3,757,184 - +0.7 3,757,184 - +0.2 3,757,184 - -0.8 3,757,184 - -0.6 3,757,184 - +1.2 3,757,184 - +0.6 3,757,184 - +1.7 3,757,184 - +0.2 3,757,207 - -0.9 3,757,254 + -0.1 3,757,254 + -0.7 3,757,254 + +0.2 3,757,254 + -1.1 3,757,254 + -1.0 3,757,254 + +0.7 3,757,255 - -0.4 3,757,255 - +2.0 3,757,255 - -1.7 3,757,290 + +0.4 3,757,290 + -0.8 3,757,290 + -0.4 3,757,291 - +0.4 3,757,291 - -0.2 3,757,299 + -1.1 3,757,300 - +0.5 3,757,352 + -1.1 3,757,353 - -1.7 3,757,353 - -0.2 3,757,353 - -0.8 3,757,356 + +1.4 3,757,357 - -0.0 3,757,410 + Pf6N2E2_3248 0.13 +0.5 3,757,410 + Pf6N2E2_3248 0.13 +2.0 3,757,410 + Pf6N2E2_3248 0.13 -0.4 3,757,410 + Pf6N2E2_3248 0.13 +0.5 3,757,411 - Pf6N2E2_3248 0.13 -0.8 3,757,411 - Pf6N2E2_3248 0.13 -2.1 3,757,885 + -0.2 3,757,886 - -0.6 3,757,886 - +0.3 3,757,886 - -0.0 3,757,886 - +1.6 3,757,886 - -0.2 3,757,886 - +0.9 3,757,886 - -0.0 3,757,886 - +1.1 3,757,886 - -1.0 3,757,979 - -0.0 3,758,043 - -0.7 3,758,060 - +0.0 3,758,060 - +0.9
Or see this region's nucleotide sequence