Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2

Experiment: Nickel (II) chloride 0.0025 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1 and Pf6N2E2_2 are separated by 145 nucleotidesPf6N2E2_2 and Pf6N2E2_3 are separated by 273 nucleotides Pf6N2E2_1 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 90 to 860 _1 Pf6N2E2_2 - FIG057993:Thioesterase involved in non-ribosomal peptide biosynthesis, at 1,006 to 1,740 _2 Pf6N2E2_3 - Non-ribosomal peptide synthetase modules, pyoverdine, at 2,014 to 14,454 _3 Position (kb) 1 2Strain fitness (log2 ratio) -2 -1 0 1at 0.027 kb on + strandat 0.028 kb on - strandat 0.035 kb on - strandat 0.035 kb on - strandat 0.158 kb on - strandat 0.181 kb on + strand, within Pf6N2E2_1at 0.182 kb on - strand, within Pf6N2E2_1at 0.193 kb on + strand, within Pf6N2E2_1at 0.298 kb on + strand, within Pf6N2E2_1at 0.349 kb on + strand, within Pf6N2E2_1at 0.353 kb on - strand, within Pf6N2E2_1at 0.353 kb on - strand, within Pf6N2E2_1at 0.369 kb on + strand, within Pf6N2E2_1at 0.370 kb on - strand, within Pf6N2E2_1at 0.370 kb on - strand, within Pf6N2E2_1at 0.838 kb on + strandat 0.839 kb on - strandat 0.839 kb on - strandat 0.839 kb on - strandat 0.902 kb on + strandat 0.902 kb on + strandat 0.902 kb on + strandat 0.903 kb on - strandat 0.904 kb on + strandat 0.904 kb on + strandat 0.904 kb on + strandat 0.904 kb on + strandat 0.905 kb on - strandat 0.905 kb on - strandat 0.948 kb on - strandat 0.955 kb on + strandat 0.955 kb on + strandat 1.037 kb on - strandat 1.060 kb on + strandat 1.060 kb on + strandat 1.060 kb on + strandat 1.061 kb on - strandat 1.349 kb on - strand, within Pf6N2E2_2at 1.430 kb on + strand, within Pf6N2E2_2at 1.431 kb on - strand, within Pf6N2E2_2at 1.431 kb on - strand, within Pf6N2E2_2at 1.431 kb on - strand, within Pf6N2E2_2at 1.960 kb on - strandat 2.173 kb on + strandat 2.227 kb on + strandat 2.228 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.0025 M
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27 + -0.7
28 - +0.5
35 - +0.4
35 - -0.5
158 - -1.8
181 + Pf6N2E2_1 0.12 +0.4
182 - Pf6N2E2_1 0.12 -0.7
193 + Pf6N2E2_1 0.13 -0.3
298 + Pf6N2E2_1 0.27 -0.7
349 + Pf6N2E2_1 0.34 +0.1
353 - Pf6N2E2_1 0.34 -0.8
353 - Pf6N2E2_1 0.34 -2.1
369 + Pf6N2E2_1 0.36 -1.6
370 - Pf6N2E2_1 0.36 +0.3
370 - Pf6N2E2_1 0.36 +1.7
838 + -0.8
839 - +0.5
839 - -1.5
839 - +1.6
902 + -0.1
902 + +1.3
902 + -0.0
903 - -0.9
904 + -0.0
904 + -0.9
904 + -0.9
904 + -0.3
905 - -0.1
905 - -0.2
948 - +1.4
955 + -0.2
955 + +0.7
1,037 - +0.2
1,060 + +0.1
1,060 + -0.2
1,060 + -1.4
1,061 - +0.8
1,349 - Pf6N2E2_2 0.47 -1.2
1,430 + Pf6N2E2_2 0.58 -0.8
1,431 - Pf6N2E2_2 0.58 -0.7
1,431 - Pf6N2E2_2 0.58 +0.2
1,431 - Pf6N2E2_2 0.58 -0.3
1,960 - +0.3
2,173 + +0.3
2,227 + +0.2
2,228 - +1.1

Or see this region's nucleotide sequence