Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3603 and Shew_3604 are separated by 294 nucleotides Shew_3604 and Shew_3605 are separated by 14 nucleotides Shew_3605 and Shew_3606 are separated by 83 nucleotides
Shew_3603: Shew_3603 - RNA polymerase factor sigma-32 (RefSeq), at 4,301,446 to 4,302,303
_3603
Shew_3604: Shew_3604 - hypothetical protein (RefSeq), at 4,302,598 to 4,303,563
_3604
Shew_3605: Shew_3605 - cell division ATP-binding protein FtsE (RefSeq), at 4,303,578 to 4,304,279
_3605
Shew_3606: Shew_3606 - signal recognition particle-docking protein FtsY (RefSeq), at 4,304,363 to 4,306,048
_3606
Position (kb)
4302
4303
4304 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 4302.592 kb on - strand at 4302.675 kb on - strand at 4302.824 kb on - strand, within Shew_3604 at 4303.133 kb on - strand, within Shew_3604 at 4303.176 kb on - strand, within Shew_3604 at 4303.176 kb on - strand, within Shew_3604 at 4303.177 kb on - strand, within Shew_3604 at 4303.281 kb on + strand, within Shew_3604 at 4303.286 kb on - strand, within Shew_3604 at 4303.350 kb on + strand, within Shew_3604 at 4303.406 kb on - strand, within Shew_3604 at 4303.406 kb on - strand, within Shew_3604 at 4303.469 kb on - strand at 4303.469 kb on - strand at 4303.569 kb on - strand at 4303.634 kb on + strand at 4303.646 kb on + strand at 4303.951 kb on - strand, within Shew_3605 at 4304.164 kb on + strand, within Shew_3605 at 4304.232 kb on + strand at 4304.253 kb on - strand at 4304.254 kb on + strand at 4304.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 4,302,592 - +0.5 4,302,675 - -6.3 4,302,824 - Shew_3604 0.23 -4.3 4,303,133 - Shew_3604 0.55 -4.4 4,303,176 - Shew_3604 0.60 -3.6 4,303,176 - Shew_3604 0.60 -0.6 4,303,177 - Shew_3604 0.60 -5.1 4,303,281 + Shew_3604 0.71 -7.1 4,303,286 - Shew_3604 0.71 -5.4 4,303,350 + Shew_3604 0.78 -5.7 4,303,406 - Shew_3604 0.84 -5.1 4,303,406 - Shew_3604 0.84 -5.3 4,303,469 - -4.4 4,303,469 - -2.5 4,303,569 - +1.2 4,303,634 + -3.7 4,303,646 + -0.5 4,303,951 - Shew_3605 0.53 -1.9 4,304,164 + Shew_3605 0.83 -3.3 4,304,232 + -3.9 4,304,253 - -5.1 4,304,254 + -3.0 4,304,337 + -3.7
Or see this region's nucleotide sequence