Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3466 and Shew_3467 are separated by 11 nucleotides Shew_3467 and Shew_3468 are separated by 226 nucleotides
Shew_3466: Shew_3466 - glycerol kinase (RefSeq), at 4,149,031 to 4,150,527
_3466
Shew_3467: Shew_3467 - hypothetical protein (RefSeq), at 4,150,539 to 4,151,036
_3467
Shew_3468: Shew_3468 - membrane protein involved in aromatic hydrocarbon degradation (RefSeq), at 4,151,263 to 4,152,618
_3468
Position (kb)
4150
4151
4152 Strain fitness (log2 ratio)
-2
-1
0
1 at 4149.555 kb on - strand, within Shew_3466 at 4149.764 kb on + strand, within Shew_3466 at 4149.947 kb on + strand, within Shew_3466 at 4149.996 kb on + strand, within Shew_3466 at 4150.035 kb on - strand, within Shew_3466 at 4150.108 kb on - strand, within Shew_3466 at 4150.266 kb on + strand, within Shew_3466 at 4150.495 kb on - strand at 4150.552 kb on + strand at 4150.618 kb on + strand, within Shew_3467 at 4150.741 kb on - strand, within Shew_3467 at 4150.884 kb on + strand, within Shew_3467 at 4150.939 kb on - strand, within Shew_3467 at 4151.194 kb on + strand at 4151.238 kb on - strand at 4151.238 kb on - strand at 4151.494 kb on - strand, within Shew_3468 at 4151.556 kb on + strand, within Shew_3468 at 4151.651 kb on + strand, within Shew_3468 at 4151.831 kb on - strand, within Shew_3468
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 4,149,555 - Shew_3466 0.35 -2.0 4,149,764 + Shew_3466 0.49 -0.7 4,149,947 + Shew_3466 0.61 +0.3 4,149,996 + Shew_3466 0.64 +0.4 4,150,035 - Shew_3466 0.67 +0.7 4,150,108 - Shew_3466 0.72 +1.0 4,150,266 + Shew_3466 0.82 +0.3 4,150,495 - -0.4 4,150,552 + -0.7 4,150,618 + Shew_3467 0.16 -1.1 4,150,741 - Shew_3467 0.41 +0.8 4,150,884 + Shew_3467 0.69 +0.9 4,150,939 - Shew_3467 0.80 +1.1 4,151,194 + -0.3 4,151,238 - -0.6 4,151,238 - +0.3 4,151,494 - Shew_3468 0.17 +0.0 4,151,556 + Shew_3468 0.22 -0.9 4,151,651 + Shew_3468 0.29 -0.0 4,151,831 - Shew_3468 0.42 +1.1
Or see this region's nucleotide sequence