Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2643 and Shew_2644 are separated by 10 nucleotides Shew_2644 and Shew_2645 are separated by 183 nucleotides Shew_2645 and Shew_2646 overlap by 4 nucleotides
Shew_2643: Shew_2643 - pseudouridylate synthase (RefSeq), at 3,118,781 to 3,119,602
_2643
Shew_2644: Shew_2644 - hypothetical protein (RefSeq), at 3,119,613 to 3,119,930
_2644
Shew_2645: Shew_2645 - hypothetical protein (RefSeq), at 3,120,114 to 3,121,127
_2645
Shew_2646: Shew_2646 - hypothetical protein (RefSeq), at 3,121,124 to 3,121,513
_2646
Position (kb)
3120
3121
3122 Strain fitness (log2 ratio)
-1
0
1 at 3119.245 kb on + strand, within Shew_2643 at 3119.717 kb on - strand, within Shew_2644 at 3119.814 kb on + strand, within Shew_2644 at 3120.009 kb on + strand at 3120.224 kb on + strand, within Shew_2645 at 3120.250 kb on - strand, within Shew_2645 at 3120.451 kb on - strand, within Shew_2645 at 3120.508 kb on - strand, within Shew_2645 at 3120.584 kb on - strand, within Shew_2645 at 3120.589 kb on - strand, within Shew_2645 at 3120.598 kb on + strand, within Shew_2645 at 3120.961 kb on - strand, within Shew_2645 at 3121.083 kb on - strand at 3121.106 kb on + strand at 3121.286 kb on - strand, within Shew_2646 at 3121.970 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 3,119,245 + Shew_2643 0.56 -0.9 3,119,717 - Shew_2644 0.33 -0.3 3,119,814 + Shew_2644 0.63 +0.2 3,120,009 + -0.5 3,120,224 + Shew_2645 0.11 +0.4 3,120,250 - Shew_2645 0.13 -1.2 3,120,451 - Shew_2645 0.33 +0.1 3,120,508 - Shew_2645 0.39 -0.7 3,120,584 - Shew_2645 0.46 +0.3 3,120,589 - Shew_2645 0.47 -0.9 3,120,598 + Shew_2645 0.48 -0.3 3,120,961 - Shew_2645 0.84 -0.2 3,121,083 - -0.0 3,121,106 + +1.4 3,121,286 - Shew_2646 0.42 -0.5 3,121,970 - +1.7
Or see this region's nucleotide sequence