Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2541 and Shew_2542 are separated by 100 nucleotides Shew_2542 and Shew_2543 are separated by 133 nucleotides Shew_2543 and Shew_2544 are separated by 168 nucleotides
Shew_2541: Shew_2541 - helix-turn-helix, type 11 domain-containing protein (RefSeq), at 2,997,867 to 2,998,553
_2541
Shew_2542: Shew_2542 - transcription activator, effector binding (RefSeq), at 2,998,654 to 2,999,112
_2542
Shew_2543: Shew_2543 - glyoxalase family protein (RefSeq), at 2,999,246 to 2,999,665
_2543
Shew_2544: Shew_2544 - phosphoglucomutase/phosphomannomutase alpha/beta/subunit (RefSeq), at 2,999,834 to 3,001,588
_2544
Position (kb)
2999
3000 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2998.471 kb on - strand, within Shew_2541 at 2998.548 kb on + strand at 2998.636 kb on + strand at 2998.842 kb on + strand, within Shew_2542 at 2998.959 kb on - strand, within Shew_2542 at 2999.014 kb on - strand, within Shew_2542 at 2999.410 kb on + strand, within Shew_2543 at 2999.411 kb on - strand, within Shew_2543 at 2999.571 kb on + strand, within Shew_2543 at 2999.612 kb on + strand, within Shew_2543 at 2999.612 kb on + strand, within Shew_2543 at 2999.703 kb on - strand at 2999.703 kb on - strand at 2999.756 kb on - strand at 2999.897 kb on + strand at 3000.473 kb on - strand, within Shew_2544
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 2,998,471 - Shew_2541 0.88 +0.3 2,998,548 + +2.1 2,998,636 + -4.2 2,998,842 + Shew_2542 0.41 -0.2 2,998,959 - Shew_2542 0.66 +0.2 2,999,014 - Shew_2542 0.78 +0.2 2,999,410 + Shew_2543 0.39 -2.1 2,999,411 - Shew_2543 0.39 -0.5 2,999,571 + Shew_2543 0.77 +0.4 2,999,612 + Shew_2543 0.87 +0.6 2,999,612 + Shew_2543 0.87 +1.9 2,999,703 - +1.5 2,999,703 - +0.1 2,999,756 - +0.0 2,999,897 + +0.2 3,000,473 - Shew_2544 0.36 -1.9
Or see this region's nucleotide sequence