Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2422 and Shew_2423 are separated by 18 nucleotides Shew_2423 and Shew_2424 are separated by 293 nucleotides Shew_2424 and fadJ are separated by 158 nucleotides fadJ and fadI are separated by 4 nucleotides
Shew_2422: Shew_2422 - hypothetical protein (RefSeq), at 2,847,897 to 2,848,532
_2422
Shew_2423: Shew_2423 - hypothetical protein (RefSeq), at 2,848,551 to 2,848,670
_2423
Shew_2424: Shew_2424 - hypothetical protein (RefSeq), at 2,848,964 to 2,849,170
_2424
Shew_2425: fadJ - multifunctional fatty acid oxidation complex subunit alpha (RefSeq), at 2,849,329 to 2,851,449
fadJ
Shew_2426: fadI - 3-ketoacyl-CoA thiolase (RefSeq), at 2,851,454 to 2,852,764
fadI
Position (kb)
2849
2850
2851
2852 Strain fitness (log2 ratio)
-2
-1
0
1 at 2848.360 kb on - strand, within Shew_2422 at 2848.621 kb on - strand, within Shew_2423 at 2848.819 kb on - strand at 2848.855 kb on + strand at 2848.954 kb on - strand at 2850.004 kb on - strand, within fadJ at 2850.300 kb on + strand, within fadJ at 2850.402 kb on - strand, within fadJ at 2850.414 kb on - strand, within fadJ at 2850.495 kb on - strand, within fadJ at 2850.597 kb on - strand, within fadJ at 2850.790 kb on - strand, within fadJ at 2851.278 kb on - strand at 2851.587 kb on - strand, within fadI at 2851.723 kb on - strand, within fadI at 2851.982 kb on + strand, within fadI
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 2,848,360 - Shew_2422 0.73 +0.4 2,848,621 - Shew_2423 0.58 +0.1 2,848,819 - -0.1 2,848,855 + +0.6 2,848,954 - -1.6 2,850,004 - fadJ Shew_2425 0.32 -0.1 2,850,300 + fadJ Shew_2425 0.46 +0.2 2,850,402 - fadJ Shew_2425 0.51 +0.8 2,850,414 - fadJ Shew_2425 0.51 +0.4 2,850,495 - fadJ Shew_2425 0.55 +0.5 2,850,597 - fadJ Shew_2425 0.60 +0.2 2,850,790 - fadJ Shew_2425 0.69 +0.3 2,851,278 - -1.9 2,851,587 - fadI Shew_2426 0.10 -0.6 2,851,723 - fadI Shew_2426 0.21 +0.1 2,851,982 + fadI Shew_2426 0.40 -0.8
Or see this region's nucleotide sequence