Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and Shew_2076 are separated by 19 nucleotides Shew_2076 and Shew_2077 overlap by 4 nucleotides Shew_2077 and Shew_2078 are separated by 7 nucleotides Shew_2078 and aspS are separated by 70 nucleotides
Shew_2075: ruvB - Holliday junction DNA helicase B (RefSeq), at 2,399,610 to 2,400,623
ruvB
Shew_2076: Shew_2076 - Holliday junction DNA helicase RuvA (RefSeq), at 2,400,643 to 2,401,260
_2076
Shew_2077: Shew_2077 - crossover junction endodeoxyribonuclease RuvC (RefSeq), at 2,401,257 to 2,401,778
_2077
Shew_2078: Shew_2078 - hypothetical protein (RefSeq), at 2,401,786 to 2,402,532
_2078
Shew_2079: aspS - aspartyl-tRNA synthetase (RefSeq), at 2,402,603 to 2,404,381
aspS
Position (kb)
2401
2402 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2400.409 kb on + strand, within ruvB at 2400.409 kb on + strand, within ruvB at 2401.337 kb on + strand, within Shew_2077 at 2401.407 kb on + strand, within Shew_2077 at 2401.719 kb on - strand, within Shew_2077 at 2401.987 kb on - strand, within Shew_2078
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 2,400,409 + ruvB Shew_2075 0.79 -2.5 2,400,409 + ruvB Shew_2075 0.79 -1.9 2,401,337 + Shew_2077 0.15 -1.9 2,401,407 + Shew_2077 0.29 -1.5 2,401,719 - Shew_2077 0.89 -5.6 2,401,987 - Shew_2078 0.27 -2.1
Or see this region's nucleotide sequence