Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1475 and Shew_1476 are separated by 57 nucleotides Shew_1476 and Shew_1477 overlap by 8 nucleotides Shew_1477 and Shew_1478 are separated by 49 nucleotides Shew_1478 and Shew_1479 overlap by 4 nucleotides
Shew_1475: Shew_1475 - hypothetical protein (RefSeq), at 1,700,959 to 1,701,648
_1475
Shew_1476: Shew_1476 - RNA polymerase sigma factor (RefSeq), at 1,701,706 to 1,702,344
_1476
Shew_1477: Shew_1477 - anti-ECFsigma factor, ChrR (RefSeq), at 1,702,337 to 1,703,086
_1477
Shew_1478: Shew_1478 - hypothetical protein (RefSeq), at 1,703,136 to 1,704,080
_1478
Shew_1479: Shew_1479 - deoxyribodipyrimidine photo-lyase (RefSeq), at 1,704,077 to 1,705,513
_1479
Position (kb)
1702
1703
1704 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1701.413 kb on + strand, within Shew_1475 at 1701.744 kb on - strand at 1701.933 kb on - strand, within Shew_1476 at 1702.007 kb on - strand, within Shew_1476 at 1702.203 kb on + strand, within Shew_1476 at 1702.743 kb on + strand, within Shew_1477 at 1702.941 kb on - strand, within Shew_1477 at 1703.045 kb on - strand at 1703.331 kb on + strand, within Shew_1478 at 1703.345 kb on - strand, within Shew_1478
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 1,701,413 + Shew_1475 0.66 +0.1 1,701,744 - -0.9 1,701,933 - Shew_1476 0.36 -1.0 1,702,007 - Shew_1476 0.47 -1.1 1,702,203 + Shew_1476 0.78 +0.9 1,702,743 + Shew_1477 0.54 -1.8 1,702,941 - Shew_1477 0.81 -3.7 1,703,045 - -1.7 1,703,331 + Shew_1478 0.21 +0.3 1,703,345 - Shew_1478 0.22 -0.0
Or see this region's nucleotide sequence