Experiment: Motility M3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhF and Shew_1382 overlap by 26 nucleotides Shew_1382 and fliA overlap by 8 nucleotides fliA and Shew_1384 are separated by 42 nucleotides Shew_1384 and Shew_1385 are separated by 13 nucleotides
Shew_1381: flhF - flagellar biosynthesis regulator FlhF (RefSeq), at 1,590,430 to 1,591,854
flhF
Shew_1382: Shew_1382 - cobyrinic acid a,c-diamide synthase (RefSeq), at 1,591,829 to 1,592,740
_1382
Shew_1383: fliA - flagellar biosynthesis sigma factor (RefSeq), at 1,592,733 to 1,593,452
fliA
Shew_1384: Shew_1384 - response regulator receiver protein (RefSeq), at 1,593,495 to 1,593,878
_1384
Shew_1385: Shew_1385 - chemotaxis phosphatase, CheZ (RefSeq), at 1,593,892 to 1,594,629
_1385
Position (kb)
1592
1593
1594 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1591.736 kb on + strand at 1592.030 kb on + strand, within Shew_1382 at 1592.144 kb on - strand, within Shew_1382 at 1592.213 kb on + strand, within Shew_1382 at 1592.872 kb on - strand, within fliA at 1592.874 kb on + strand, within fliA at 1593.035 kb on - strand, within fliA at 1593.695 kb on + strand, within Shew_1384 at 1593.905 kb on + strand at 1593.930 kb on + strand at 1594.241 kb on - strand, within Shew_1385 at 1594.277 kb on + strand, within Shew_1385 at 1594.323 kb on - strand, within Shew_1385 at 1594.410 kb on - strand, within Shew_1385 at 1594.450 kb on + strand, within Shew_1385
Per-strain Table
Position Strand Gene LocusTag Fraction Motility M3 remove 1,591,736 + -6.2 1,592,030 + Shew_1382 0.22 -2.9 1,592,144 - Shew_1382 0.35 -2.8 1,592,213 + Shew_1382 0.42 -3.0 1,592,872 - fliA Shew_1383 0.19 -7.3 1,592,874 + fliA Shew_1383 0.20 -4.7 1,593,035 - fliA Shew_1383 0.42 -5.4 1,593,695 + Shew_1384 0.52 -2.3 1,593,905 + -3.6 1,593,930 + -1.9 1,594,241 - Shew_1385 0.47 -6.2 1,594,277 + Shew_1385 0.52 -8.0 1,594,323 - Shew_1385 0.58 -4.8 1,594,410 - Shew_1385 0.70 -3.4 1,594,450 + Shew_1385 0.76 -6.5
Or see this region's nucleotide sequence