Experiment: MoYLS4 with DETA/NO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0125 and b3486 overlap by 4 nucleotides b3486 and DVU0127 are separated by 3 nucleotides
DVU0125: DVU0125 - hypothetical protein (TIGR), at 158,213 to 159,346
DVU0125
DVU0126: b3486 - ABC transporter, ATP-binding protein (TIGR), at 159,343 to 162,858
b3486
DVU0127: DVU0127 - membrane protein, putative (TIGR), at 162,862 to 163,974
DVU0127
Position (kb) 159
160
161
162
163 Strain fitness (log2 ratio) -2
-1
0
1
2 at 158.357 kb on - strand, within DVU0125 at 158.386 kb on - strand, within DVU0125 at 158.428 kb on + strand, within DVU0125 at 158.443 kb on + strand, within DVU0125 at 158.486 kb on + strand, within DVU0125 at 158.503 kb on - strand, within DVU0125 at 158.525 kb on - strand, within DVU0125 at 158.579 kb on + strand, within DVU0125 at 158.698 kb on + strand, within DVU0125 at 158.706 kb on - strand, within DVU0125 at 158.706 kb on - strand, within DVU0125 at 158.706 kb on - strand, within DVU0125 at 158.816 kb on - strand, within DVU0125 at 158.908 kb on - strand, within DVU0125 at 158.972 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.092 kb on - strand, within DVU0125 at 159.092 kb on - strand, within DVU0125 at 159.092 kb on - strand, within DVU0125 at 159.099 kb on + strand, within DVU0125 at 159.101 kb on + strand, within DVU0125 at 159.135 kb on + strand, within DVU0125 at 159.136 kb on + strand, within DVU0125 at 159.171 kb on + strand, within DVU0125 at 159.194 kb on + strand, within DVU0125 at 159.220 kb on + strand, within DVU0125 at 159.228 kb on - strand, within DVU0125 at 159.279 kb on + strand at 159.290 kb on + strand at 159.290 kb on + strand at 159.408 kb on - strand at 159.431 kb on - strand at 159.469 kb on - strand at 159.549 kb on + strand at 159.572 kb on - strand at 159.584 kb on + strand at 159.649 kb on - strand at 159.772 kb on + strand, within b3486 at 159.780 kb on - strand, within b3486 at 159.810 kb on - strand, within b3486 at 159.810 kb on - strand, within b3486 at 159.817 kb on + strand, within b3486 at 159.833 kb on - strand, within b3486 at 159.836 kb on + strand, within b3486 at 159.836 kb on + strand, within b3486 at 159.866 kb on - strand, within b3486 at 159.940 kb on + strand, within b3486 at 159.985 kb on - strand, within b3486 at 159.990 kb on + strand, within b3486 at 159.990 kb on + strand, within b3486 at 159.998 kb on - strand, within b3486 at 160.008 kb on - strand, within b3486 at 160.021 kb on - strand, within b3486 at 160.057 kb on - strand, within b3486 at 160.057 kb on - strand, within b3486 at 160.057 kb on - strand, within b3486 at 160.062 kb on + strand, within b3486 at 160.129 kb on - strand, within b3486 at 160.142 kb on - strand, within b3486 at 160.155 kb on - strand, within b3486 at 160.155 kb on - strand, within b3486 at 160.156 kb on + strand, within b3486 at 160.158 kb on + strand, within b3486 at 160.159 kb on + strand, within b3486 at 160.192 kb on + strand, within b3486 at 160.227 kb on + strand, within b3486 at 160.227 kb on + strand, within b3486 at 160.235 kb on - strand, within b3486 at 160.235 kb on - strand, within b3486 at 160.235 kb on - strand, within b3486 at 160.237 kb on + strand, within b3486 at 160.245 kb on - strand, within b3486 at 160.245 kb on - strand, within b3486 at 160.355 kb on + strand, within b3486 at 160.366 kb on + strand, within b3486 at 160.366 kb on + strand, within b3486 at 160.455 kb on + strand, within b3486 at 160.580 kb on - strand, within b3486 at 160.605 kb on + strand, within b3486 at 160.615 kb on - strand, within b3486 at 160.631 kb on - strand, within b3486 at 160.631 kb on - strand, within b3486 at 160.631 kb on - strand, within b3486 at 160.648 kb on - strand, within b3486 at 160.657 kb on - strand, within b3486 at 160.689 kb on - strand, within b3486 at 160.694 kb on - strand, within b3486 at 160.694 kb on - strand, within b3486 at 160.696 kb on + strand, within b3486 at 160.742 kb on - strand, within b3486 at 160.783 kb on - strand, within b3486 at 160.823 kb on + strand, within b3486 at 160.832 kb on + strand, within b3486 at 160.872 kb on + strand, within b3486 at 160.880 kb on - strand, within b3486 at 161.071 kb on + strand, within b3486 at 161.192 kb on + strand, within b3486 at 161.228 kb on + strand, within b3486 at 161.440 kb on - strand, within b3486 at 161.446 kb on - strand, within b3486 at 161.477 kb on - strand, within b3486 at 161.482 kb on + strand, within b3486 at 161.488 kb on - strand, within b3486 at 161.492 kb on + strand, within b3486 at 161.525 kb on - strand, within b3486 at 161.544 kb on + strand, within b3486 at 161.631 kb on - strand, within b3486 at 161.679 kb on + strand, within b3486 at 161.687 kb on - strand, within b3486 at 161.700 kb on + strand, within b3486 at 161.730 kb on + strand, within b3486 at 161.781 kb on - strand, within b3486 at 161.841 kb on + strand, within b3486 at 161.935 kb on + strand, within b3486 at 162.060 kb on + strand, within b3486 at 162.068 kb on + strand, within b3486 at 162.102 kb on - strand, within b3486 at 162.113 kb on + strand, within b3486 at 162.128 kb on + strand, within b3486 at 162.183 kb on + strand, within b3486 at 162.211 kb on - strand, within b3486 at 162.211 kb on - strand, within b3486 at 162.227 kb on + strand, within b3486 at 162.306 kb on + strand, within b3486 at 162.323 kb on + strand, within b3486 at 162.428 kb on - strand, within b3486 at 162.446 kb on + strand, within b3486 at 162.446 kb on + strand, within b3486 at 162.454 kb on - strand, within b3486 at 162.554 kb on + strand at 162.564 kb on + strand at 162.564 kb on + strand at 162.636 kb on - strand at 162.664 kb on - strand at 162.673 kb on + strand at 162.692 kb on - strand at 162.702 kb on - strand at 162.741 kb on - strand at 162.821 kb on + strand at 162.903 kb on + strand at 162.905 kb on + strand at 162.915 kb on + strand at 162.915 kb on + strand at 162.923 kb on - strand at 162.923 kb on - strand at 162.960 kb on + strand at 163.057 kb on + strand, within DVU0127 at 163.076 kb on - strand, within DVU0127 at 163.133 kb on - strand, within DVU0127 at 163.150 kb on - strand, within DVU0127 at 163.154 kb on + strand, within DVU0127 at 163.159 kb on + strand, within DVU0127 at 163.160 kb on - strand, within DVU0127 at 163.277 kb on - strand, within DVU0127 at 163.303 kb on + strand, within DVU0127 at 163.303 kb on + strand, within DVU0127 at 163.303 kb on + strand, within DVU0127 at 163.325 kb on - strand, within DVU0127 at 163.364 kb on + strand, within DVU0127 at 163.364 kb on + strand, within DVU0127 at 163.372 kb on - strand, within DVU0127 at 163.434 kb on + strand, within DVU0127 at 163.471 kb on - strand, within DVU0127 at 163.486 kb on - strand, within DVU0127 at 163.496 kb on - strand, within DVU0127 at 163.511 kb on - strand, within DVU0127 at 163.574 kb on - strand, within DVU0127 at 163.578 kb on + strand, within DVU0127 at 163.591 kb on - strand, within DVU0127 at 163.719 kb on - strand, within DVU0127 at 163.759 kb on - strand, within DVU0127 at 163.791 kb on - strand, within DVU0127 at 163.818 kb on + strand, within DVU0127 at 163.825 kb on + strand, within DVU0127 at 163.826 kb on - strand, within DVU0127
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DETA/NO 100uM remove 158,357 - DVU0125 0.13 -0.4 158,386 - DVU0125 0.15 -0.6 158,428 + DVU0125 0.19 -0.7 158,443 + DVU0125 0.20 +0.7 158,486 + DVU0125 0.24 +0.2 158,503 - DVU0125 0.26 -0.3 158,525 - DVU0125 0.28 +0.5 158,579 + DVU0125 0.32 +0.6 158,698 + DVU0125 0.43 -0.5 158,706 - DVU0125 0.43 -0.1 158,706 - DVU0125 0.43 +0.4 158,706 - DVU0125 0.43 -0.5 158,816 - DVU0125 0.53 -0.3 158,908 - DVU0125 0.61 -0.4 158,972 + DVU0125 0.67 -0.0 159,084 + DVU0125 0.77 +1.6 159,084 + DVU0125 0.77 -0.4 159,084 + DVU0125 0.77 -0.5 159,084 + DVU0125 0.77 +1.4 159,092 - DVU0125 0.78 -0.1 159,092 - DVU0125 0.78 -0.9 159,092 - DVU0125 0.78 -0.6 159,099 + DVU0125 0.78 -0.6 159,101 + DVU0125 0.78 -0.5 159,135 + DVU0125 0.81 -2.5 159,136 + DVU0125 0.81 -0.7 159,171 + DVU0125 0.84 -0.6 159,194 + DVU0125 0.87 -0.2 159,220 + DVU0125 0.89 +0.2 159,228 - DVU0125 0.90 -0.1 159,279 + -0.3 159,290 + -0.5 159,290 + -0.7 159,408 - +0.3 159,431 - +2.2 159,469 - +0.4 159,549 + -0.5 159,572 - -0.7 159,584 + -0.6 159,649 - -1.1 159,772 + b3486 DVU0126 0.12 +0.0 159,780 - b3486 DVU0126 0.12 -0.4 159,810 - b3486 DVU0126 0.13 +0.2 159,810 - b3486 DVU0126 0.13 -0.9 159,817 + b3486 DVU0126 0.13 +0.4 159,833 - b3486 DVU0126 0.14 +0.4 159,836 + b3486 DVU0126 0.14 -0.6 159,836 + b3486 DVU0126 0.14 +2.0 159,866 - b3486 DVU0126 0.15 +0.4 159,940 + b3486 DVU0126 0.17 -0.2 159,985 - b3486 DVU0126 0.18 +0.0 159,990 + b3486 DVU0126 0.18 +0.2 159,990 + b3486 DVU0126 0.18 -0.4 159,998 - b3486 DVU0126 0.19 -0.0 160,008 - b3486 DVU0126 0.19 -0.3 160,021 - b3486 DVU0126 0.19 +0.1 160,057 - b3486 DVU0126 0.20 -0.6 160,057 - b3486 DVU0126 0.20 -0.8 160,057 - b3486 DVU0126 0.20 -0.6 160,062 + b3486 DVU0126 0.20 -0.4 160,129 - b3486 DVU0126 0.22 -0.2 160,142 - b3486 DVU0126 0.23 -0.6 160,155 - b3486 DVU0126 0.23 -0.1 160,155 - b3486 DVU0126 0.23 -0.4 160,156 + b3486 DVU0126 0.23 -0.2 160,158 + b3486 DVU0126 0.23 -1.1 160,159 + b3486 DVU0126 0.23 -0.6 160,192 + b3486 DVU0126 0.24 -0.4 160,227 + b3486 DVU0126 0.25 +0.4 160,227 + b3486 DVU0126 0.25 -0.0 160,235 - b3486 DVU0126 0.25 -0.4 160,235 - b3486 DVU0126 0.25 +0.3 160,235 - b3486 DVU0126 0.25 +1.1 160,237 + b3486 DVU0126 0.25 -0.4 160,245 - b3486 DVU0126 0.26 -0.0 160,245 - b3486 DVU0126 0.26 -0.2 160,355 + b3486 DVU0126 0.29 +0.0 160,366 + b3486 DVU0126 0.29 -0.4 160,366 + b3486 DVU0126 0.29 -2.1 160,455 + b3486 DVU0126 0.32 +0.4 160,580 - b3486 DVU0126 0.35 -0.9 160,605 + b3486 DVU0126 0.36 -0.5 160,615 - b3486 DVU0126 0.36 -0.2 160,631 - b3486 DVU0126 0.37 +1.0 160,631 - b3486 DVU0126 0.37 +0.7 160,631 - b3486 DVU0126 0.37 +0.0 160,648 - b3486 DVU0126 0.37 -0.5 160,657 - b3486 DVU0126 0.37 +0.4 160,689 - b3486 DVU0126 0.38 +0.1 160,694 - b3486 DVU0126 0.38 +1.4 160,694 - b3486 DVU0126 0.38 +1.4 160,696 + b3486 DVU0126 0.38 -0.3 160,742 - b3486 DVU0126 0.40 +0.0 160,783 - b3486 DVU0126 0.41 -0.4 160,823 + b3486 DVU0126 0.42 -0.4 160,832 + b3486 DVU0126 0.42 -0.6 160,872 + b3486 DVU0126 0.43 -0.7 160,880 - b3486 DVU0126 0.44 +0.3 161,071 + b3486 DVU0126 0.49 -0.6 161,192 + b3486 DVU0126 0.53 -0.8 161,228 + b3486 DVU0126 0.54 -0.1 161,440 - b3486 DVU0126 0.60 -1.0 161,446 - b3486 DVU0126 0.60 -0.7 161,477 - b3486 DVU0126 0.61 +0.1 161,482 + b3486 DVU0126 0.61 -0.4 161,488 - b3486 DVU0126 0.61 -0.6 161,492 + b3486 DVU0126 0.61 +0.4 161,525 - b3486 DVU0126 0.62 -0.4 161,544 + b3486 DVU0126 0.63 -0.2 161,631 - b3486 DVU0126 0.65 -1.1 161,679 + b3486 DVU0126 0.66 -0.7 161,687 - b3486 DVU0126 0.67 +1.4 161,700 + b3486 DVU0126 0.67 -0.1 161,730 + b3486 DVU0126 0.68 -0.1 161,781 - b3486 DVU0126 0.69 -0.6 161,841 + b3486 DVU0126 0.71 +0.7 161,935 + b3486 DVU0126 0.74 +0.3 162,060 + b3486 DVU0126 0.77 -0.2 162,068 + b3486 DVU0126 0.78 -1.6 162,102 - b3486 DVU0126 0.78 -0.2 162,113 + b3486 DVU0126 0.79 -0.4 162,128 + b3486 DVU0126 0.79 -0.0 162,183 + b3486 DVU0126 0.81 -0.4 162,211 - b3486 DVU0126 0.82 -0.1 162,211 - b3486 DVU0126 0.82 -0.4 162,227 + b3486 DVU0126 0.82 -0.3 162,306 + b3486 DVU0126 0.84 +0.4 162,323 + b3486 DVU0126 0.85 -0.9 162,428 - b3486 DVU0126 0.88 -0.5 162,446 + b3486 DVU0126 0.88 +0.4 162,446 + b3486 DVU0126 0.88 -0.3 162,454 - b3486 DVU0126 0.88 -0.8 162,554 + -1.0 162,564 + -1.8 162,564 + +0.1 162,636 - -1.1 162,664 - -0.2 162,673 + -0.9 162,692 - -0.0 162,702 - -0.2 162,741 - -0.9 162,821 + +0.0 162,903 + -0.3 162,905 + -0.1 162,915 + -0.3 162,915 + -0.1 162,923 - +0.2 162,923 - +0.0 162,960 + -0.9 163,057 + DVU0127 0.18 -0.3 163,076 - DVU0127 0.19 -0.2 163,133 - DVU0127 0.24 -0.4 163,150 - DVU0127 0.26 -1.0 163,154 + DVU0127 0.26 -0.6 163,159 + DVU0127 0.27 -0.1 163,160 - DVU0127 0.27 +0.7 163,277 - DVU0127 0.37 -0.3 163,303 + DVU0127 0.40 +0.4 163,303 + DVU0127 0.40 -0.4 163,303 + DVU0127 0.40 -0.9 163,325 - DVU0127 0.42 -0.8 163,364 + DVU0127 0.45 -0.2 163,364 + DVU0127 0.45 -0.4 163,372 - DVU0127 0.46 -0.3 163,434 + DVU0127 0.51 -0.9 163,471 - DVU0127 0.55 -0.5 163,486 - DVU0127 0.56 -0.5 163,496 - DVU0127 0.57 -0.9 163,511 - DVU0127 0.58 -0.2 163,574 - DVU0127 0.64 -0.7 163,578 + DVU0127 0.64 -2.0 163,591 - DVU0127 0.65 -0.1 163,719 - DVU0127 0.77 +0.4 163,759 - DVU0127 0.81 -0.9 163,791 - DVU0127 0.83 -0.7 163,818 + DVU0127 0.86 -0.2 163,825 + DVU0127 0.87 -1.1 163,826 - DVU0127 0.87 -2.5
Or see this region's nucleotide sequence