Experiment: L-Malic Acid (C) and N2; no light
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2881 and Rru_AR0053 are separated by 90 nucleotides Rru_AR0053 and Rru_A2882 are separated by 60 nucleotides Rru_A2882 and Rru_A2883 are separated by 225 nucleotides
Rru_A2881: Rru_A2881 - virulence protein (NCBI), at 3,320,745 to 3,322,115
_A2881
Rru_AR0053: Rru_AR0053 - tRNA-Met (NCBI), at 3,322,206 to 3,322,281
_AR0053
Rru_A2882: Rru_A2882 - Sigma 70 (RpoD) (NCBI), at 3,322,342 to 3,324,432
_A2882
Rru_A2883: Rru_A2883 - DNA primase (NCBI), at 3,324,658 to 3,326,520
_A2883
Position (kb)
3322
3323
3324
3325 Strain fitness (log2 ratio)
-2
-1
0
1 at 3321.689 kb on + strand, within Rru_A2881 at 3321.689 kb on + strand, within Rru_A2881 at 3321.689 kb on + strand, within Rru_A2881 at 3321.689 kb on + strand, within Rru_A2881 at 3321.689 kb on + strand, within Rru_A2881 at 3321.690 kb on - strand, within Rru_A2881 at 3321.690 kb on - strand, within Rru_A2881 at 3321.690 kb on - strand, within Rru_A2881 at 3321.690 kb on - strand, within Rru_A2881 at 3321.693 kb on + strand, within Rru_A2881 at 3321.693 kb on + strand, within Rru_A2881 at 3321.694 kb on - strand, within Rru_A2881 at 3321.946 kb on - strand, within Rru_A2881 at 3321.946 kb on - strand, within Rru_A2881 at 3321.946 kb on - strand, within Rru_A2881 at 3322.041 kb on + strand at 3322.041 kb on + strand at 3322.041 kb on + strand at 3322.041 kb on + strand at 3322.042 kb on - strand at 3322.042 kb on - strand at 3322.114 kb on - strand at 3322.114 kb on - strand at 3322.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic Acid (C) and N2; no light remove 3,321,689 + Rru_A2881 0.69 +0.0 3,321,689 + Rru_A2881 0.69 +0.0 3,321,689 + Rru_A2881 0.69 +0.0 3,321,689 + Rru_A2881 0.69 +0.0 3,321,689 + Rru_A2881 0.69 +0.0 3,321,690 - Rru_A2881 0.69 +0.0 3,321,690 - Rru_A2881 0.69 +0.0 3,321,690 - Rru_A2881 0.69 +0.0 3,321,690 - Rru_A2881 0.69 +0.0 3,321,693 + Rru_A2881 0.69 -1.0 3,321,693 + Rru_A2881 0.69 -1.1 3,321,694 - Rru_A2881 0.69 -1.0 3,321,946 - Rru_A2881 0.88 +0.0 3,321,946 - Rru_A2881 0.88 +0.0 3,321,946 - Rru_A2881 0.88 -1.0 3,322,041 + +0.0 3,322,041 + +0.0 3,322,041 + -1.2 3,322,041 + -1.9 3,322,042 - +0.0 3,322,042 - +0.0 3,322,114 - +0.0 3,322,114 - -1.0 3,322,114 - +0.0
Or see this region's nucleotide sequence