Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_890 and Pf6N2E2_891 are separated by 93 nucleotides Pf6N2E2_891 and Pf6N2E2_892 are separated by 72 nucleotides Pf6N2E2_892 and Pf6N2E2_893 overlap by 4 nucleotides
Pf6N2E2_890 - Nitrilotriacetate monooxygenase component B (EC 1.14.13.-), at 1,044,733 to 1,045,353
_890
Pf6N2E2_891 - 4-oxalocrotonate tautomerase, at 1,045,447 to 1,045,623
_891
Pf6N2E2_892 - hypothetical protein, at 1,045,696 to 1,045,920
_892
Pf6N2E2_893 - hypothetical protein, at 1,045,917 to 1,046,153
_893
Position (kb)
1045
1046
1047 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1045.114 kb on + strand, within Pf6N2E2_890 at 1045.201 kb on + strand, within Pf6N2E2_890 at 1045.201 kb on + strand, within Pf6N2E2_890 at 1045.230 kb on + strand, within Pf6N2E2_890 at 1045.235 kb on - strand, within Pf6N2E2_890 at 1045.235 kb on - strand, within Pf6N2E2_890 at 1045.353 kb on + strand at 1045.358 kb on + strand at 1045.359 kb on - strand at 1045.359 kb on - strand at 1045.359 kb on - strand at 1045.422 kb on - strand at 1045.448 kb on + strand at 1045.457 kb on - strand at 1045.532 kb on + strand, within Pf6N2E2_891 at 1045.532 kb on + strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.533 kb on - strand, within Pf6N2E2_891 at 1045.889 kb on + strand, within Pf6N2E2_892 at 1045.892 kb on + strand, within Pf6N2E2_892 at 1045.918 kb on + strand at 1045.918 kb on + strand at 1045.919 kb on - strand at 1045.919 kb on - strand at 1045.919 kb on - strand at 1045.919 kb on - strand at 1046.016 kb on - strand, within Pf6N2E2_893 at 1046.101 kb on + strand, within Pf6N2E2_893 at 1046.102 kb on - strand, within Pf6N2E2_893 at 1046.102 kb on - strand, within Pf6N2E2_893 at 1046.150 kb on - strand at 1046.150 kb on - strand at 1046.321 kb on + strand at 1046.339 kb on - strand at 1046.339 kb on - strand at 1046.339 kb on - strand at 1046.382 kb on - strand at 1046.557 kb on + strand at 1046.557 kb on + strand at 1046.558 kb on - strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.578 kb on + strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.579 kb on - strand at 1046.658 kb on - strand at 1046.761 kb on - strand at 1046.768 kb on - strand at 1046.807 kb on + strand at 1046.808 kb on - strand at 1046.808 kb on - strand at 1046.912 kb on + strand at 1046.912 kb on + strand at 1046.912 kb on + strand at 1047.004 kb on + strand at 1047.004 kb on + strand at 1047.005 kb on - strand at 1047.005 kb on - strand at 1047.053 kb on + strand at 1047.053 kb on + strand at 1047.054 kb on - strand at 1047.103 kb on + strand at 1047.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 1,045,114 + Pf6N2E2_890 0.61 -1.1 1,045,201 + Pf6N2E2_890 0.75 -1.8 1,045,201 + Pf6N2E2_890 0.75 +0.5 1,045,230 + Pf6N2E2_890 0.80 +0.9 1,045,235 - Pf6N2E2_890 0.81 -1.1 1,045,235 - Pf6N2E2_890 0.81 -0.3 1,045,353 + -0.4 1,045,358 + -0.1 1,045,359 - -0.0 1,045,359 - -0.6 1,045,359 - +0.2 1,045,422 - -2.5 1,045,448 + +0.2 1,045,457 - -2.0 1,045,532 + Pf6N2E2_891 0.48 -2.2 1,045,532 + Pf6N2E2_891 0.48 +1.1 1,045,533 - Pf6N2E2_891 0.49 -0.3 1,045,533 - Pf6N2E2_891 0.49 -0.7 1,045,533 - Pf6N2E2_891 0.49 -0.3 1,045,533 - Pf6N2E2_891 0.49 -0.7 1,045,533 - Pf6N2E2_891 0.49 +0.8 1,045,889 + Pf6N2E2_892 0.86 -0.2 1,045,892 + Pf6N2E2_892 0.87 +1.1 1,045,918 + -0.4 1,045,918 + +0.7 1,045,919 - -0.5 1,045,919 - -0.6 1,045,919 - +1.1 1,045,919 - -0.3 1,046,016 - Pf6N2E2_893 0.42 -1.3 1,046,101 + Pf6N2E2_893 0.78 -1.0 1,046,102 - Pf6N2E2_893 0.78 -0.7 1,046,102 - Pf6N2E2_893 0.78 +0.4 1,046,150 - -0.9 1,046,150 - +0.7 1,046,321 + -0.0 1,046,339 - -1.3 1,046,339 - -0.7 1,046,339 - -0.5 1,046,382 - +1.2 1,046,557 + -1.4 1,046,557 + -0.2 1,046,558 - -0.3 1,046,578 + +0.4 1,046,578 + +2.9 1,046,578 + -0.7 1,046,578 + -0.3 1,046,578 + -0.6 1,046,578 + -1.9 1,046,578 + +0.7 1,046,578 + -0.3 1,046,578 + -0.9 1,046,578 + -0.3 1,046,578 + -1.0 1,046,578 + -0.2 1,046,579 - +0.3 1,046,579 - -0.5 1,046,579 - +1.3 1,046,579 - -0.0 1,046,579 - -0.2 1,046,658 - -0.9 1,046,761 - +1.4 1,046,768 - -0.0 1,046,807 + +0.5 1,046,808 - -0.1 1,046,808 - -0.2 1,046,912 + +0.3 1,046,912 + +0.4 1,046,912 + -1.1 1,047,004 + +0.4 1,047,004 + -0.7 1,047,005 - -0.2 1,047,005 - +0.1 1,047,053 + -0.2 1,047,053 + -0.2 1,047,054 - +1.3 1,047,103 + -0.7 1,047,103 + +0.3
Or see this region's nucleotide sequence