Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_77 and Pf6N2E2_78 overlap by 4 nucleotides Pf6N2E2_78 and Pf6N2E2_6100 are separated by 168 nucleotides Pf6N2E2_6100 and Pf6N2E2_6101 are separated by 61 nucleotides Pf6N2E2_6101 and Pf6N2E2_6102 are separated by 93 nucleotides Pf6N2E2_6102 and Pf6N2E2_79 are separated by 432 nucleotides
Pf6N2E2_77 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 119,551 to 120,762
_77
Pf6N2E2_78 - Oxidoreductase, short chain dehydrogenase/reductase family, at 120,759 to 121,532
_78
Pf6N2E2_6100 - tRNA-Val-TAC, at 121,701 to 121,773
_6100
Pf6N2E2_6101 - tRNA-Asp-GTC, at 121,835 to 121,908
_6101
Pf6N2E2_6102 - tRNA-Asp-GTC, at 122,002 to 122,075
_6102
Pf6N2E2_79 - Mobile element protein, at 122,508 to 123,776
_79
Position (kb)
120
121
122 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 119.944 kb on + strand, within Pf6N2E2_77 at 119.945 kb on - strand, within Pf6N2E2_77 at 119.974 kb on + strand, within Pf6N2E2_77 at 120.024 kb on + strand, within Pf6N2E2_77 at 120.439 kb on + strand, within Pf6N2E2_77 at 121.092 kb on + strand, within Pf6N2E2_78 at 121.159 kb on - strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.236 kb on + strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.237 kb on - strand, within Pf6N2E2_78 at 121.412 kb on - strand, within Pf6N2E2_78 at 121.421 kb on - strand, within Pf6N2E2_78 at 121.524 kb on + strand at 121.524 kb on + strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.525 kb on - strand at 121.572 kb on + strand at 121.572 kb on + strand at 121.573 kb on - strand at 121.598 kb on + strand at 121.599 kb on - strand at 121.658 kb on - strand at 121.734 kb on + strand, within Pf6N2E2_6100 at 121.734 kb on + strand, within Pf6N2E2_6100 at 121.782 kb on + strand at 122.139 kb on + strand at 122.203 kb on + strand at 122.203 kb on + strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.252 kb on - strand at 122.256 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 119,944 + Pf6N2E2_77 0.32 +0.8 119,945 - Pf6N2E2_77 0.33 +0.7 119,974 + Pf6N2E2_77 0.35 +0.4 120,024 + Pf6N2E2_77 0.39 -0.4 120,439 + Pf6N2E2_77 0.73 +0.1 121,092 + Pf6N2E2_78 0.43 -1.3 121,159 - Pf6N2E2_78 0.52 -0.7 121,236 + Pf6N2E2_78 0.62 -0.2 121,236 + Pf6N2E2_78 0.62 -2.3 121,237 - Pf6N2E2_78 0.62 -0.1 121,237 - Pf6N2E2_78 0.62 -1.7 121,412 - Pf6N2E2_78 0.84 -0.7 121,421 - Pf6N2E2_78 0.86 -0.7 121,524 + +0.8 121,524 + +1.4 121,525 - +0.4 121,525 - -0.6 121,525 - -0.4 121,572 + -0.0 121,572 + -0.4 121,573 - -1.4 121,598 + -0.2 121,599 - -0.9 121,658 - +0.3 121,734 + Pf6N2E2_6100 0.45 -0.3 121,734 + Pf6N2E2_6100 0.45 -0.4 121,782 + +1.1 122,139 + -0.7 122,203 + +0.5 122,203 + +0.3 122,252 - -1.2 122,252 - -0.0 122,252 - -1.5 122,252 - +2.8 122,252 - +0.5 122,256 - +1.0
Or see this region's nucleotide sequence