Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4239 and Pf6N2E2_4240 overlap by 1 nucleotides Pf6N2E2_4240 and Pf6N2E2_4241 overlap by 10 nucleotides Pf6N2E2_4241 and Pf6N2E2_4242 are separated by 3 nucleotides Pf6N2E2_4242 and Pf6N2E2_4243 overlap by 4 nucleotides
Pf6N2E2_4239 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 4,931,024 to 4,932,289
_4239
Pf6N2E2_4240 - O-antigen export system permease protein RfbD, at 4,932,289 to 4,933,086
_4240
Pf6N2E2_4241 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22), at 4,933,077 to 4,934,528
_4241
Pf6N2E2_4242 - GDP-mannose 4,6-dehydratase (EC 4.2.1.47), at 4,934,532 to 4,935,500
_4242
Pf6N2E2_4243 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 4,935,497 to 4,936,321
_4243
Position (kb)
4933
4934
4935 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4932.253 kb on - strand at 4932.253 kb on - strand at 4932.350 kb on - strand at 4932.350 kb on - strand at 4932.350 kb on - strand at 4932.407 kb on + strand, within Pf6N2E2_4240 at 4932.407 kb on + strand, within Pf6N2E2_4240 at 4932.407 kb on + strand, within Pf6N2E2_4240 at 4932.407 kb on + strand, within Pf6N2E2_4240 at 4932.408 kb on - strand, within Pf6N2E2_4240 at 4932.425 kb on + strand, within Pf6N2E2_4240 at 4932.425 kb on + strand, within Pf6N2E2_4240 at 4932.425 kb on + strand, within Pf6N2E2_4240 at 4932.426 kb on - strand, within Pf6N2E2_4240 at 4932.654 kb on + strand, within Pf6N2E2_4240 at 4932.654 kb on + strand, within Pf6N2E2_4240 at 4932.654 kb on + strand, within Pf6N2E2_4240 at 4932.654 kb on + strand, within Pf6N2E2_4240 at 4932.655 kb on - strand, within Pf6N2E2_4240 at 4932.655 kb on - strand, within Pf6N2E2_4240 at 4932.655 kb on - strand, within Pf6N2E2_4240 at 4932.869 kb on + strand, within Pf6N2E2_4240 at 4932.869 kb on + strand, within Pf6N2E2_4240 at 4932.870 kb on - strand, within Pf6N2E2_4240 at 4932.870 kb on - strand, within Pf6N2E2_4240 at 4932.878 kb on + strand, within Pf6N2E2_4240 at 4932.878 kb on + strand, within Pf6N2E2_4240 at 4932.879 kb on - strand, within Pf6N2E2_4240 at 4932.879 kb on - strand, within Pf6N2E2_4240 at 4932.941 kb on + strand, within Pf6N2E2_4240 at 4933.042 kb on - strand at 4933.042 kb on - strand at 4933.060 kb on + strand at 4933.067 kb on + strand at 4933.067 kb on + strand at 4933.067 kb on + strand at 4933.102 kb on + strand at 4933.103 kb on - strand at 4933.141 kb on + strand at 4933.141 kb on + strand at 4933.142 kb on - strand at 4933.142 kb on - strand at 4933.142 kb on - strand at 4933.401 kb on - strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.402 kb on + strand, within Pf6N2E2_4241 at 4933.403 kb on - strand, within Pf6N2E2_4241 at 4933.403 kb on - strand, within Pf6N2E2_4241 at 4933.403 kb on - strand, within Pf6N2E2_4241 at 4933.403 kb on - strand, within Pf6N2E2_4241 at 4933.403 kb on - strand, within Pf6N2E2_4241 at 4933.427 kb on + strand, within Pf6N2E2_4241 at 4933.428 kb on - strand, within Pf6N2E2_4241 at 4933.428 kb on - strand, within Pf6N2E2_4241 at 4933.428 kb on - strand, within Pf6N2E2_4241 at 4933.613 kb on + strand, within Pf6N2E2_4241 at 4933.753 kb on + strand, within Pf6N2E2_4241 at 4933.753 kb on + strand, within Pf6N2E2_4241 at 4933.753 kb on + strand, within Pf6N2E2_4241 at 4933.754 kb on - strand, within Pf6N2E2_4241 at 4933.754 kb on - strand, within Pf6N2E2_4241 at 4933.754 kb on - strand, within Pf6N2E2_4241 at 4933.754 kb on - strand, within Pf6N2E2_4241 at 4933.836 kb on - strand, within Pf6N2E2_4241 at 4933.963 kb on + strand, within Pf6N2E2_4241 at 4934.053 kb on + strand, within Pf6N2E2_4241 at 4934.053 kb on + strand, within Pf6N2E2_4241 at 4934.054 kb on - strand, within Pf6N2E2_4241 at 4934.054 kb on - strand, within Pf6N2E2_4241 at 4934.086 kb on - strand, within Pf6N2E2_4241 at 4934.190 kb on + strand, within Pf6N2E2_4241 at 4934.321 kb on - strand, within Pf6N2E2_4241 at 4934.341 kb on + strand, within Pf6N2E2_4241 at 4934.341 kb on + strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.342 kb on - strand, within Pf6N2E2_4241 at 4934.344 kb on + strand, within Pf6N2E2_4241 at 4934.345 kb on - strand, within Pf6N2E2_4241 at 4934.345 kb on - strand, within Pf6N2E2_4241 at 4934.408 kb on - strand at 4934.408 kb on - strand at 4934.439 kb on + strand at 4934.533 kb on + strand at 4934.534 kb on - strand at 4934.534 kb on - strand at 4934.674 kb on + strand, within Pf6N2E2_4242 at 4934.675 kb on - strand, within Pf6N2E2_4242 at 4934.775 kb on + strand, within Pf6N2E2_4242 at 4934.775 kb on + strand, within Pf6N2E2_4242 at 4934.776 kb on - strand, within Pf6N2E2_4242 at 4934.776 kb on - strand, within Pf6N2E2_4242 at 4934.776 kb on - strand, within Pf6N2E2_4242 at 4934.779 kb on + strand, within Pf6N2E2_4242 at 4934.779 kb on + strand, within Pf6N2E2_4242 at 4934.779 kb on + strand, within Pf6N2E2_4242 at 4934.779 kb on + strand, within Pf6N2E2_4242 at 4934.779 kb on + strand, within Pf6N2E2_4242 at 4934.780 kb on - strand, within Pf6N2E2_4242 at 4934.780 kb on - strand, within Pf6N2E2_4242 at 4934.827 kb on + strand, within Pf6N2E2_4242 at 4934.828 kb on - strand, within Pf6N2E2_4242 at 4934.828 kb on - strand, within Pf6N2E2_4242 at 4934.828 kb on - strand, within Pf6N2E2_4242 at 4935.013 kb on + strand, within Pf6N2E2_4242 at 4935.014 kb on - strand, within Pf6N2E2_4242 at 4935.038 kb on - strand, within Pf6N2E2_4242 at 4935.140 kb on - strand, within Pf6N2E2_4242 at 4935.149 kb on + strand, within Pf6N2E2_4242 at 4935.149 kb on + strand, within Pf6N2E2_4242 at 4935.150 kb on - strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.268 kb on + strand, within Pf6N2E2_4242 at 4935.269 kb on - strand, within Pf6N2E2_4242 at 4935.269 kb on - strand, within Pf6N2E2_4242 at 4935.269 kb on - strand, within Pf6N2E2_4242 at 4935.424 kb on + strand at 4935.454 kb on + strand at 4935.454 kb on + strand at 4935.455 kb on - strand at 4935.509 kb on + strand at 4935.509 kb on + strand at 4935.510 kb on - strand at 4935.510 kb on - strand at 4935.516 kb on - strand at 4935.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 4,932,253 - +0.4 4,932,253 - -0.4 4,932,350 - +0.7 4,932,350 - +1.0 4,932,350 - -1.0 4,932,407 + Pf6N2E2_4240 0.15 -1.5 4,932,407 + Pf6N2E2_4240 0.15 +0.3 4,932,407 + Pf6N2E2_4240 0.15 -0.7 4,932,407 + Pf6N2E2_4240 0.15 +0.7 4,932,408 - Pf6N2E2_4240 0.15 +0.9 4,932,425 + Pf6N2E2_4240 0.17 +1.0 4,932,425 + Pf6N2E2_4240 0.17 +0.2 4,932,425 + Pf6N2E2_4240 0.17 +1.5 4,932,426 - Pf6N2E2_4240 0.17 +1.9 4,932,654 + Pf6N2E2_4240 0.46 -0.4 4,932,654 + Pf6N2E2_4240 0.46 +0.2 4,932,654 + Pf6N2E2_4240 0.46 -0.8 4,932,654 + Pf6N2E2_4240 0.46 -0.3 4,932,655 - Pf6N2E2_4240 0.46 -0.1 4,932,655 - Pf6N2E2_4240 0.46 -0.7 4,932,655 - Pf6N2E2_4240 0.46 -0.7 4,932,869 + Pf6N2E2_4240 0.73 +1.0 4,932,869 + Pf6N2E2_4240 0.73 +0.1 4,932,870 - Pf6N2E2_4240 0.73 +0.5 4,932,870 - Pf6N2E2_4240 0.73 -0.1 4,932,878 + Pf6N2E2_4240 0.74 -0.4 4,932,878 + Pf6N2E2_4240 0.74 -1.8 4,932,879 - Pf6N2E2_4240 0.74 +0.1 4,932,879 - Pf6N2E2_4240 0.74 +0.8 4,932,941 + Pf6N2E2_4240 0.82 -0.4 4,933,042 - -1.7 4,933,042 - -0.6 4,933,060 + +0.2 4,933,067 + -1.2 4,933,067 + -1.4 4,933,067 + -0.7 4,933,102 + -1.0 4,933,103 - +0.9 4,933,141 + +0.4 4,933,141 + +1.6 4,933,142 - -1.1 4,933,142 - +0.0 4,933,142 - -0.6 4,933,401 - Pf6N2E2_4241 0.22 -0.1 4,933,402 + Pf6N2E2_4241 0.22 -0.6 4,933,402 + Pf6N2E2_4241 0.22 +1.3 4,933,402 + Pf6N2E2_4241 0.22 -0.2 4,933,402 + Pf6N2E2_4241 0.22 -0.4 4,933,402 + Pf6N2E2_4241 0.22 +0.4 4,933,402 + Pf6N2E2_4241 0.22 +0.9 4,933,402 + Pf6N2E2_4241 0.22 -0.1 4,933,402 + Pf6N2E2_4241 0.22 +1.1 4,933,402 + Pf6N2E2_4241 0.22 -0.0 4,933,402 + Pf6N2E2_4241 0.22 -1.6 4,933,402 + Pf6N2E2_4241 0.22 +0.1 4,933,402 + Pf6N2E2_4241 0.22 +0.0 4,933,402 + Pf6N2E2_4241 0.22 +0.2 4,933,403 - Pf6N2E2_4241 0.22 +0.4 4,933,403 - Pf6N2E2_4241 0.22 +0.5 4,933,403 - Pf6N2E2_4241 0.22 -0.3 4,933,403 - Pf6N2E2_4241 0.22 +0.2 4,933,403 - Pf6N2E2_4241 0.22 -0.1 4,933,427 + Pf6N2E2_4241 0.24 +0.2 4,933,428 - Pf6N2E2_4241 0.24 +0.3 4,933,428 - Pf6N2E2_4241 0.24 +1.1 4,933,428 - Pf6N2E2_4241 0.24 +0.3 4,933,613 + Pf6N2E2_4241 0.37 +0.3 4,933,753 + Pf6N2E2_4241 0.47 +0.6 4,933,753 + Pf6N2E2_4241 0.47 +0.3 4,933,753 + Pf6N2E2_4241 0.47 -0.3 4,933,754 - Pf6N2E2_4241 0.47 +1.2 4,933,754 - Pf6N2E2_4241 0.47 +1.2 4,933,754 - Pf6N2E2_4241 0.47 -0.2 4,933,754 - Pf6N2E2_4241 0.47 -1.0 4,933,836 - Pf6N2E2_4241 0.52 +1.6 4,933,963 + Pf6N2E2_4241 0.61 +0.6 4,934,053 + Pf6N2E2_4241 0.67 -0.8 4,934,053 + Pf6N2E2_4241 0.67 -0.1 4,934,054 - Pf6N2E2_4241 0.67 -1.3 4,934,054 - Pf6N2E2_4241 0.67 -0.3 4,934,086 - Pf6N2E2_4241 0.69 -0.7 4,934,190 + Pf6N2E2_4241 0.77 -1.0 4,934,321 - Pf6N2E2_4241 0.86 +1.1 4,934,341 + Pf6N2E2_4241 0.87 -0.6 4,934,341 + Pf6N2E2_4241 0.87 +0.1 4,934,342 - Pf6N2E2_4241 0.87 -1.0 4,934,342 - Pf6N2E2_4241 0.87 -2.0 4,934,342 - Pf6N2E2_4241 0.87 -0.1 4,934,342 - Pf6N2E2_4241 0.87 +1.0 4,934,342 - Pf6N2E2_4241 0.87 +0.2 4,934,342 - Pf6N2E2_4241 0.87 +0.7 4,934,342 - Pf6N2E2_4241 0.87 -0.5 4,934,344 + Pf6N2E2_4241 0.87 +0.1 4,934,345 - Pf6N2E2_4241 0.87 +0.6 4,934,345 - Pf6N2E2_4241 0.87 +0.2 4,934,408 - +0.8 4,934,408 - -2.0 4,934,439 + -0.8 4,934,533 + +0.1 4,934,534 - +0.4 4,934,534 - -0.6 4,934,674 + Pf6N2E2_4242 0.15 +1.4 4,934,675 - Pf6N2E2_4242 0.15 -0.1 4,934,775 + Pf6N2E2_4242 0.25 -0.5 4,934,775 + Pf6N2E2_4242 0.25 +0.2 4,934,776 - Pf6N2E2_4242 0.25 +0.6 4,934,776 - Pf6N2E2_4242 0.25 -0.5 4,934,776 - Pf6N2E2_4242 0.25 +0.0 4,934,779 + Pf6N2E2_4242 0.25 -0.0 4,934,779 + Pf6N2E2_4242 0.25 -0.1 4,934,779 + Pf6N2E2_4242 0.25 +0.6 4,934,779 + Pf6N2E2_4242 0.25 -0.5 4,934,779 + Pf6N2E2_4242 0.25 +0.0 4,934,780 - Pf6N2E2_4242 0.26 -2.5 4,934,780 - Pf6N2E2_4242 0.26 +1.0 4,934,827 + Pf6N2E2_4242 0.30 +1.2 4,934,828 - Pf6N2E2_4242 0.31 -0.3 4,934,828 - Pf6N2E2_4242 0.31 +0.2 4,934,828 - Pf6N2E2_4242 0.31 -0.3 4,935,013 + Pf6N2E2_4242 0.50 -0.2 4,935,014 - Pf6N2E2_4242 0.50 +0.8 4,935,038 - Pf6N2E2_4242 0.52 +1.0 4,935,140 - Pf6N2E2_4242 0.63 +0.2 4,935,149 + Pf6N2E2_4242 0.64 -0.5 4,935,149 + Pf6N2E2_4242 0.64 +0.5 4,935,150 - Pf6N2E2_4242 0.64 -0.4 4,935,268 + Pf6N2E2_4242 0.76 -0.6 4,935,268 + Pf6N2E2_4242 0.76 -0.7 4,935,268 + Pf6N2E2_4242 0.76 -1.7 4,935,268 + Pf6N2E2_4242 0.76 +1.2 4,935,268 + Pf6N2E2_4242 0.76 -0.2 4,935,268 + Pf6N2E2_4242 0.76 +0.4 4,935,268 + Pf6N2E2_4242 0.76 -0.6 4,935,269 - Pf6N2E2_4242 0.76 -0.2 4,935,269 - Pf6N2E2_4242 0.76 +0.1 4,935,269 - Pf6N2E2_4242 0.76 +1.0 4,935,424 + +0.6 4,935,454 + +1.0 4,935,454 + -0.5 4,935,455 - +0.2 4,935,509 + -0.1 4,935,509 + -0.3 4,935,510 - -0.6 4,935,510 - -0.3 4,935,516 - +1.4 4,935,516 - -1.0
Or see this region's nucleotide sequence