Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2796 and Pf6N2E2_2797 are separated by 247 nucleotides Pf6N2E2_2797 and Pf6N2E2_2798 overlap by 11 nucleotides Pf6N2E2_2798 and Pf6N2E2_2799 are separated by 29 nucleotides Pf6N2E2_2799 and Pf6N2E2_2800 are separated by 257 nucleotides
Pf6N2E2_2796 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 3,282,988 to 3,284,337
_2796
Pf6N2E2_2797 - Prevent host death protein, Phd antitoxin, at 3,284,585 to 3,284,824
_2797
Pf6N2E2_2798 - Death on curing protein, Doc toxin, at 3,284,814 to 3,285,098
_2798
Pf6N2E2_2799 - Fosfomycin resistance protein FosA, at 3,285,128 to 3,285,541
_2799
Pf6N2E2_2800 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 3,285,799 to 3,287,034
_2800
Position (kb)
3284
3285
3286 Strain fitness (log2 ratio)
-1
0
1
2 at 3283.845 kb on - strand, within Pf6N2E2_2796 at 3283.845 kb on - strand, within Pf6N2E2_2796 at 3283.845 kb on - strand, within Pf6N2E2_2796 at 3283.888 kb on - strand, within Pf6N2E2_2796 at 3283.888 kb on - strand, within Pf6N2E2_2796 at 3283.956 kb on - strand, within Pf6N2E2_2796 at 3284.185 kb on + strand, within Pf6N2E2_2796 at 3284.186 kb on - strand, within Pf6N2E2_2796 at 3284.186 kb on - strand, within Pf6N2E2_2796 at 3284.214 kb on - strand at 3284.303 kb on + strand at 3284.303 kb on + strand at 3284.303 kb on + strand at 3284.304 kb on - strand at 3284.304 kb on - strand at 3284.304 kb on - strand at 3284.406 kb on - strand at 3284.406 kb on - strand at 3284.406 kb on - strand at 3284.434 kb on - strand at 3284.465 kb on + strand at 3284.466 kb on - strand at 3284.547 kb on + strand at 3284.547 kb on + strand at 3284.548 kb on - strand at 3284.550 kb on + strand at 3284.550 kb on + strand at 3284.551 kb on - strand at 3284.560 kb on + strand at 3284.697 kb on - strand, within Pf6N2E2_2797 at 3284.697 kb on - strand, within Pf6N2E2_2797 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.869 kb on - strand, within Pf6N2E2_2798 at 3284.871 kb on - strand, within Pf6N2E2_2798 at 3284.871 kb on - strand, within Pf6N2E2_2798 at 3284.871 kb on - strand, within Pf6N2E2_2798 at 3285.137 kb on - strand at 3285.137 kb on - strand at 3285.137 kb on - strand at 3285.156 kb on + strand at 3285.156 kb on + strand at 3285.206 kb on + strand, within Pf6N2E2_2799 at 3285.206 kb on + strand, within Pf6N2E2_2799 at 3285.206 kb on + strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.207 kb on - strand, within Pf6N2E2_2799 at 3285.345 kb on + strand, within Pf6N2E2_2799 at 3285.354 kb on + strand, within Pf6N2E2_2799 at 3285.354 kb on + strand, within Pf6N2E2_2799 at 3285.355 kb on - strand, within Pf6N2E2_2799 at 3285.426 kb on + strand, within Pf6N2E2_2799 at 3285.427 kb on - strand, within Pf6N2E2_2799 at 3285.427 kb on - strand, within Pf6N2E2_2799 at 3285.464 kb on + strand, within Pf6N2E2_2799 at 3285.465 kb on - strand, within Pf6N2E2_2799 at 3285.465 kb on - strand, within Pf6N2E2_2799 at 3285.467 kb on + strand, within Pf6N2E2_2799 at 3285.467 kb on + strand, within Pf6N2E2_2799 at 3285.467 kb on + strand, within Pf6N2E2_2799 at 3285.468 kb on - strand, within Pf6N2E2_2799 at 3285.954 kb on + strand, within Pf6N2E2_2800 at 3285.972 kb on + strand, within Pf6N2E2_2800 at 3285.983 kb on - strand, within Pf6N2E2_2800 at 3285.997 kb on + strand, within Pf6N2E2_2800 at 3285.998 kb on - strand, within Pf6N2E2_2800
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 3,283,845 - Pf6N2E2_2796 0.63 +0.8 3,283,845 - Pf6N2E2_2796 0.63 -0.4 3,283,845 - Pf6N2E2_2796 0.63 +0.6 3,283,888 - Pf6N2E2_2796 0.67 +0.1 3,283,888 - Pf6N2E2_2796 0.67 -0.9 3,283,956 - Pf6N2E2_2796 0.72 +1.0 3,284,185 + Pf6N2E2_2796 0.89 -0.2 3,284,186 - Pf6N2E2_2796 0.89 +0.5 3,284,186 - Pf6N2E2_2796 0.89 -1.0 3,284,214 - -0.6 3,284,303 + -0.8 3,284,303 + +1.4 3,284,303 + +0.2 3,284,304 - -0.5 3,284,304 - +1.0 3,284,304 - +0.0 3,284,406 - +1.7 3,284,406 - -1.0 3,284,406 - +1.6 3,284,434 - -0.1 3,284,465 + -0.3 3,284,466 - +0.4 3,284,547 + +1.2 3,284,547 + +0.8 3,284,548 - +0.0 3,284,550 + +2.3 3,284,550 + +1.5 3,284,551 - -0.0 3,284,560 + -0.6 3,284,697 - Pf6N2E2_2797 0.47 +0.6 3,284,697 - Pf6N2E2_2797 0.47 +2.1 3,284,869 - Pf6N2E2_2798 0.19 +0.1 3,284,869 - Pf6N2E2_2798 0.19 -0.3 3,284,869 - Pf6N2E2_2798 0.19 -1.4 3,284,869 - Pf6N2E2_2798 0.19 -0.8 3,284,869 - Pf6N2E2_2798 0.19 +0.7 3,284,871 - Pf6N2E2_2798 0.20 -0.6 3,284,871 - Pf6N2E2_2798 0.20 -0.6 3,284,871 - Pf6N2E2_2798 0.20 -0.1 3,285,137 - -0.2 3,285,137 - -0.9 3,285,137 - -0.4 3,285,156 + +0.9 3,285,156 + -0.8 3,285,206 + Pf6N2E2_2799 0.19 -0.7 3,285,206 + Pf6N2E2_2799 0.19 -0.3 3,285,206 + Pf6N2E2_2799 0.19 -1.4 3,285,207 - Pf6N2E2_2799 0.19 -1.5 3,285,207 - Pf6N2E2_2799 0.19 -0.3 3,285,207 - Pf6N2E2_2799 0.19 +0.6 3,285,207 - Pf6N2E2_2799 0.19 -0.1 3,285,207 - Pf6N2E2_2799 0.19 +0.3 3,285,207 - Pf6N2E2_2799 0.19 +0.1 3,285,207 - Pf6N2E2_2799 0.19 +1.8 3,285,207 - Pf6N2E2_2799 0.19 +1.1 3,285,345 + Pf6N2E2_2799 0.52 -0.6 3,285,354 + Pf6N2E2_2799 0.55 -0.2 3,285,354 + Pf6N2E2_2799 0.55 +0.7 3,285,355 - Pf6N2E2_2799 0.55 +0.1 3,285,426 + Pf6N2E2_2799 0.72 +2.1 3,285,427 - Pf6N2E2_2799 0.72 -1.1 3,285,427 - Pf6N2E2_2799 0.72 +1.0 3,285,464 + Pf6N2E2_2799 0.81 +0.8 3,285,465 - Pf6N2E2_2799 0.81 -0.8 3,285,465 - Pf6N2E2_2799 0.81 +0.2 3,285,467 + Pf6N2E2_2799 0.82 +0.8 3,285,467 + Pf6N2E2_2799 0.82 +1.0 3,285,467 + Pf6N2E2_2799 0.82 +1.4 3,285,468 - Pf6N2E2_2799 0.82 +0.4 3,285,954 + Pf6N2E2_2800 0.13 +0.2 3,285,972 + Pf6N2E2_2800 0.14 -0.2 3,285,983 - Pf6N2E2_2800 0.15 +0.7 3,285,997 + Pf6N2E2_2800 0.16 +0.3 3,285,998 - Pf6N2E2_2800 0.16 +0.7
Or see this region's nucleotide sequence