Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2362 and Pf6N2E2_2363 overlap by 26 nucleotides Pf6N2E2_2363 and Pf6N2E2_2364 are separated by 4 nucleotides Pf6N2E2_2364 and Pf6N2E2_2365 are separated by 8 nucleotides Pf6N2E2_2365 and Pf6N2E2_2366 overlap by 8 nucleotides
Pf6N2E2_2362 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 2,797,341 to 2,798,375
_2362
Pf6N2E2_2363 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 2,798,350 to 2,799,153
_2363
Pf6N2E2_2364 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 2,799,158 to 2,799,412
_2364
Pf6N2E2_2365 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 2,799,421 to 2,800,080
_2365
Pf6N2E2_2366 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 2,800,073 to 2,800,453
_2366
Position (kb)
2798
2799
2800 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2797.417 kb on + strand at 2797.417 kb on + strand at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.570 kb on + strand, within Pf6N2E2_2362 at 2797.570 kb on + strand, within Pf6N2E2_2362 at 2797.766 kb on - strand, within Pf6N2E2_2362 at 2797.948 kb on + strand, within Pf6N2E2_2362 at 2797.949 kb on - strand, within Pf6N2E2_2362 at 2798.207 kb on - strand, within Pf6N2E2_2362 at 2798.248 kb on + strand, within Pf6N2E2_2362 at 2798.249 kb on - strand, within Pf6N2E2_2362 at 2798.465 kb on + strand, within Pf6N2E2_2363 at 2798.659 kb on + strand, within Pf6N2E2_2363 at 2798.693 kb on + strand, within Pf6N2E2_2363 at 2798.745 kb on - strand, within Pf6N2E2_2363 at 2798.908 kb on + strand, within Pf6N2E2_2363 at 2798.985 kb on - strand, within Pf6N2E2_2363 at 2799.125 kb on + strand at 2799.126 kb on - strand at 2799.159 kb on + strand at 2799.440 kb on + strand at 2799.599 kb on + strand, within Pf6N2E2_2365 at 2799.599 kb on + strand, within Pf6N2E2_2365 at 2799.599 kb on + strand, within Pf6N2E2_2365 at 2799.605 kb on + strand, within Pf6N2E2_2365 at 2799.605 kb on + strand, within Pf6N2E2_2365 at 2799.605 kb on + strand, within Pf6N2E2_2365 at 2799.606 kb on - strand, within Pf6N2E2_2365 at 2799.768 kb on - strand, within Pf6N2E2_2365 at 2799.779 kb on + strand, within Pf6N2E2_2365 at 2799.788 kb on + strand, within Pf6N2E2_2365 at 2799.789 kb on - strand, within Pf6N2E2_2365 at 2799.789 kb on - strand, within Pf6N2E2_2365 at 2799.838 kb on - strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.861 kb on - strand, within Pf6N2E2_2365 at 2799.861 kb on - strand, within Pf6N2E2_2365 at 2799.861 kb on - strand, within Pf6N2E2_2365 at 2799.864 kb on - strand, within Pf6N2E2_2365 at 2799.864 kb on - strand, within Pf6N2E2_2365 at 2800.004 kb on + strand, within Pf6N2E2_2365 at 2800.005 kb on - strand, within Pf6N2E2_2365
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 2,797,417 + +1.1 2,797,417 + -1.0 2,797,492 + Pf6N2E2_2362 0.15 -2.8 2,797,492 + Pf6N2E2_2362 0.15 -0.2 2,797,492 + Pf6N2E2_2362 0.15 -0.2 2,797,570 + Pf6N2E2_2362 0.22 -0.7 2,797,570 + Pf6N2E2_2362 0.22 -0.0 2,797,766 - Pf6N2E2_2362 0.41 +0.2 2,797,948 + Pf6N2E2_2362 0.59 +0.1 2,797,949 - Pf6N2E2_2362 0.59 -0.5 2,798,207 - Pf6N2E2_2362 0.84 -0.5 2,798,248 + Pf6N2E2_2362 0.88 +0.3 2,798,249 - Pf6N2E2_2362 0.88 -0.1 2,798,465 + Pf6N2E2_2363 0.14 -1.3 2,798,659 + Pf6N2E2_2363 0.38 -0.3 2,798,693 + Pf6N2E2_2363 0.43 -0.3 2,798,745 - Pf6N2E2_2363 0.49 -1.0 2,798,908 + Pf6N2E2_2363 0.69 -1.1 2,798,985 - Pf6N2E2_2363 0.79 +0.9 2,799,125 + -0.1 2,799,126 - -0.1 2,799,159 + +1.1 2,799,440 + -0.7 2,799,599 + Pf6N2E2_2365 0.27 +0.1 2,799,599 + Pf6N2E2_2365 0.27 -0.8 2,799,599 + Pf6N2E2_2365 0.27 -0.8 2,799,605 + Pf6N2E2_2365 0.28 +0.6 2,799,605 + Pf6N2E2_2365 0.28 -0.4 2,799,605 + Pf6N2E2_2365 0.28 +0.1 2,799,606 - Pf6N2E2_2365 0.28 +0.9 2,799,768 - Pf6N2E2_2365 0.53 -0.1 2,799,779 + Pf6N2E2_2365 0.54 +0.6 2,799,788 + Pf6N2E2_2365 0.56 +0.5 2,799,789 - Pf6N2E2_2365 0.56 +0.6 2,799,789 - Pf6N2E2_2365 0.56 -0.1 2,799,838 - Pf6N2E2_2365 0.63 +1.5 2,799,860 + Pf6N2E2_2365 0.67 +0.5 2,799,860 + Pf6N2E2_2365 0.67 +0.3 2,799,860 + Pf6N2E2_2365 0.67 -0.5 2,799,860 + Pf6N2E2_2365 0.67 -1.3 2,799,860 + Pf6N2E2_2365 0.67 +1.3 2,799,861 - Pf6N2E2_2365 0.67 +0.3 2,799,861 - Pf6N2E2_2365 0.67 +0.2 2,799,861 - Pf6N2E2_2365 0.67 -1.0 2,799,864 - Pf6N2E2_2365 0.67 -0.7 2,799,864 - Pf6N2E2_2365 0.67 +1.4 2,800,004 + Pf6N2E2_2365 0.88 -0.5 2,800,005 - Pf6N2E2_2365 0.88 +0.5
Or see this region's nucleotide sequence