Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2348 and Pf6N2E2_2349 are separated by 41 nucleotides Pf6N2E2_2349 and Pf6N2E2_2350 are separated by 4 nucleotides Pf6N2E2_2350 and Pf6N2E2_2351 overlap by 29 nucleotides Pf6N2E2_2351 and Pf6N2E2_2352 are separated by 4 nucleotides
Pf6N2E2_2348 - hypothetical protein, at 2,784,170 to 2,785,534
_2348
Pf6N2E2_2349 - hypothetical protein, at 2,785,576 to 2,785,989
_2349
Pf6N2E2_2350 - Oxygen-regulated invasion protein OrgB, at 2,785,994 to 2,786,704
_2350
Pf6N2E2_2351 - Oxygen-regulated invasion protein OrgA, at 2,786,676 to 2,787,224
_2351
Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948
_2352
Position (kb)
2785
2786
2787 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2785.013 kb on - strand, within Pf6N2E2_2348 at 2785.013 kb on - strand, within Pf6N2E2_2348 at 2785.028 kb on - strand, within Pf6N2E2_2348 at 2785.053 kb on + strand, within Pf6N2E2_2348 at 2785.053 kb on + strand, within Pf6N2E2_2348 at 2785.054 kb on - strand, within Pf6N2E2_2348 at 2785.054 kb on - strand, within Pf6N2E2_2348 at 2785.054 kb on - strand, within Pf6N2E2_2348 at 2785.217 kb on + strand, within Pf6N2E2_2348 at 2785.218 kb on - strand, within Pf6N2E2_2348 at 2785.218 kb on - strand, within Pf6N2E2_2348 at 2785.220 kb on - strand, within Pf6N2E2_2348 at 2785.220 kb on - strand, within Pf6N2E2_2348 at 2785.220 kb on - strand, within Pf6N2E2_2348 at 2785.289 kb on + strand, within Pf6N2E2_2348 at 2785.290 kb on - strand, within Pf6N2E2_2348 at 2785.290 kb on - strand, within Pf6N2E2_2348 at 2785.290 kb on - strand, within Pf6N2E2_2348 at 2785.290 kb on - strand, within Pf6N2E2_2348 at 2785.292 kb on - strand, within Pf6N2E2_2348 at 2785.315 kb on - strand, within Pf6N2E2_2348 at 2785.315 kb on - strand, within Pf6N2E2_2348 at 2785.355 kb on + strand, within Pf6N2E2_2348 at 2785.355 kb on + strand, within Pf6N2E2_2348 at 2785.355 kb on + strand, within Pf6N2E2_2348 at 2785.355 kb on + strand, within Pf6N2E2_2348 at 2785.356 kb on - strand, within Pf6N2E2_2348 at 2785.356 kb on - strand, within Pf6N2E2_2348 at 2785.449 kb on - strand at 2785.505 kb on - strand at 2785.549 kb on - strand at 2785.555 kb on - strand at 2785.555 kb on - strand at 2785.555 kb on - strand at 2785.559 kb on - strand at 2785.559 kb on - strand at 2785.559 kb on - strand at 2785.559 kb on - strand at 2785.559 kb on - strand at 2785.559 kb on - strand at 2785.642 kb on + strand, within Pf6N2E2_2349 at 2785.642 kb on + strand, within Pf6N2E2_2349 at 2785.643 kb on - strand, within Pf6N2E2_2349 at 2785.643 kb on - strand, within Pf6N2E2_2349 at 2785.643 kb on - strand, within Pf6N2E2_2349 at 2785.643 kb on - strand, within Pf6N2E2_2349 at 2785.646 kb on + strand, within Pf6N2E2_2349 at 2785.646 kb on + strand, within Pf6N2E2_2349 at 2785.647 kb on - strand, within Pf6N2E2_2349 at 2785.647 kb on - strand, within Pf6N2E2_2349 at 2785.693 kb on - strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.738 kb on + strand, within Pf6N2E2_2349 at 2785.739 kb on - strand, within Pf6N2E2_2349 at 2785.739 kb on - strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2786.205 kb on + strand, within Pf6N2E2_2350 at 2786.205 kb on + strand, within Pf6N2E2_2350 at 2786.206 kb on - strand, within Pf6N2E2_2350 at 2786.223 kb on + strand, within Pf6N2E2_2350 at 2786.224 kb on - strand, within Pf6N2E2_2350 at 2786.224 kb on - strand, within Pf6N2E2_2350 at 2786.920 kb on - strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.132 kb on + strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.193 kb on + strand at 2787.194 kb on - strand at 2787.269 kb on + strand at 2787.413 kb on + strand, within Pf6N2E2_2352 at 2787.557 kb on + strand, within Pf6N2E2_2352 at 2787.558 kb on - strand, within Pf6N2E2_2352 at 2787.635 kb on + strand, within Pf6N2E2_2352 at 2787.636 kb on - strand, within Pf6N2E2_2352
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 2,785,013 - Pf6N2E2_2348 0.62 -0.7 2,785,013 - Pf6N2E2_2348 0.62 +1.1 2,785,028 - Pf6N2E2_2348 0.63 +0.9 2,785,053 + Pf6N2E2_2348 0.65 -0.8 2,785,053 + Pf6N2E2_2348 0.65 -1.0 2,785,054 - Pf6N2E2_2348 0.65 -0.9 2,785,054 - Pf6N2E2_2348 0.65 -1.1 2,785,054 - Pf6N2E2_2348 0.65 -0.3 2,785,217 + Pf6N2E2_2348 0.77 +1.9 2,785,218 - Pf6N2E2_2348 0.77 +0.3 2,785,218 - Pf6N2E2_2348 0.77 +0.5 2,785,220 - Pf6N2E2_2348 0.77 -1.0 2,785,220 - Pf6N2E2_2348 0.77 +0.6 2,785,220 - Pf6N2E2_2348 0.77 +0.6 2,785,289 + Pf6N2E2_2348 0.82 +0.5 2,785,290 - Pf6N2E2_2348 0.82 -1.1 2,785,290 - Pf6N2E2_2348 0.82 -0.0 2,785,290 - Pf6N2E2_2348 0.82 +0.3 2,785,290 - Pf6N2E2_2348 0.82 -0.3 2,785,292 - Pf6N2E2_2348 0.82 -0.5 2,785,315 - Pf6N2E2_2348 0.84 -0.4 2,785,315 - Pf6N2E2_2348 0.84 +0.2 2,785,355 + Pf6N2E2_2348 0.87 +0.6 2,785,355 + Pf6N2E2_2348 0.87 +1.1 2,785,355 + Pf6N2E2_2348 0.87 +1.3 2,785,355 + Pf6N2E2_2348 0.87 +0.9 2,785,356 - Pf6N2E2_2348 0.87 -0.6 2,785,356 - Pf6N2E2_2348 0.87 -1.3 2,785,449 - -0.5 2,785,505 - -2.0 2,785,549 - +1.1 2,785,555 - +1.3 2,785,555 - +0.1 2,785,555 - -0.5 2,785,559 - -0.7 2,785,559 - -0.7 2,785,559 - -0.8 2,785,559 - +0.2 2,785,559 - -0.8 2,785,559 - -0.1 2,785,642 + Pf6N2E2_2349 0.16 +0.9 2,785,642 + Pf6N2E2_2349 0.16 +1.2 2,785,643 - Pf6N2E2_2349 0.16 -0.5 2,785,643 - Pf6N2E2_2349 0.16 +0.1 2,785,643 - Pf6N2E2_2349 0.16 -0.6 2,785,643 - Pf6N2E2_2349 0.16 -0.6 2,785,646 + Pf6N2E2_2349 0.17 -0.1 2,785,646 + Pf6N2E2_2349 0.17 -0.1 2,785,647 - Pf6N2E2_2349 0.17 +1.2 2,785,647 - Pf6N2E2_2349 0.17 +1.2 2,785,693 - Pf6N2E2_2349 0.28 +0.3 2,785,694 + Pf6N2E2_2349 0.29 +0.1 2,785,694 + Pf6N2E2_2349 0.29 +0.2 2,785,694 + Pf6N2E2_2349 0.29 +0.4 2,785,694 + Pf6N2E2_2349 0.29 -0.6 2,785,694 + Pf6N2E2_2349 0.29 -0.9 2,785,694 + Pf6N2E2_2349 0.29 +0.0 2,785,695 - Pf6N2E2_2349 0.29 +0.8 2,785,695 - Pf6N2E2_2349 0.29 +0.1 2,785,695 - Pf6N2E2_2349 0.29 +1.0 2,785,695 - Pf6N2E2_2349 0.29 -1.2 2,785,695 - Pf6N2E2_2349 0.29 +0.8 2,785,695 - Pf6N2E2_2349 0.29 -0.7 2,785,695 - Pf6N2E2_2349 0.29 +0.2 2,785,695 - Pf6N2E2_2349 0.29 +0.3 2,785,695 - Pf6N2E2_2349 0.29 +0.5 2,785,738 + Pf6N2E2_2349 0.39 -2.6 2,785,739 - Pf6N2E2_2349 0.39 -1.3 2,785,739 - Pf6N2E2_2349 0.39 +0.8 2,785,871 + Pf6N2E2_2349 0.71 +0.1 2,785,871 + Pf6N2E2_2349 0.71 -1.5 2,785,871 + Pf6N2E2_2349 0.71 -1.5 2,785,871 + Pf6N2E2_2349 0.71 -1.0 2,786,205 + Pf6N2E2_2350 0.30 -0.1 2,786,205 + Pf6N2E2_2350 0.30 -1.3 2,786,206 - Pf6N2E2_2350 0.30 -0.5 2,786,223 + Pf6N2E2_2350 0.32 -0.6 2,786,224 - Pf6N2E2_2350 0.32 -0.6 2,786,224 - Pf6N2E2_2350 0.32 -0.8 2,786,920 - Pf6N2E2_2351 0.44 -0.1 2,787,023 + Pf6N2E2_2351 0.63 -0.4 2,787,023 + Pf6N2E2_2351 0.63 -0.1 2,787,024 - Pf6N2E2_2351 0.63 +0.3 2,787,024 - Pf6N2E2_2351 0.63 +0.3 2,787,026 - Pf6N2E2_2351 0.64 -1.0 2,787,026 - Pf6N2E2_2351 0.64 +0.0 2,787,026 - Pf6N2E2_2351 0.64 +1.4 2,787,132 + Pf6N2E2_2351 0.83 +0.0 2,787,133 - Pf6N2E2_2351 0.83 +0.1 2,787,133 - Pf6N2E2_2351 0.83 +0.4 2,787,193 + +1.3 2,787,194 - +0.2 2,787,269 + +1.9 2,787,413 + Pf6N2E2_2352 0.26 +0.3 2,787,557 + Pf6N2E2_2352 0.46 -0.5 2,787,558 - Pf6N2E2_2352 0.46 +0.7 2,787,635 + Pf6N2E2_2352 0.56 +0.3 2,787,636 - Pf6N2E2_2352 0.57 +0.3
Or see this region's nucleotide sequence