Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_222

Experiment: Magnesium chloride 0.125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_221 and Pf6N2E2_222 are separated by 2 nucleotidesPf6N2E2_222 and Pf6N2E2_223 overlap by 4 nucleotides Pf6N2E2_221 - RND efflux system, membrane fusion protein CmeA, at 279,239 to 280,432 _221 Pf6N2E2_222 - RND efflux system, inner membrane transporter CmeB, at 280,435 to 283,581 _222 Pf6N2E2_223 - RND efflux system, outer membrane lipoprotein CmeC, at 283,578 to 284,975 _223 Position (kb) 280 281 282 283 284Strain fitness (log2 ratio) -2 -1 0 1at 279.444 kb on + strandat 279.522 kb on - strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.542 kb on + strand, within Pf6N2E2_221at 279.543 kb on - strand, within Pf6N2E2_221at 279.758 kb on + strand, within Pf6N2E2_221at 279.758 kb on + strand, within Pf6N2E2_221at 279.758 kb on + strand, within Pf6N2E2_221at 279.759 kb on - strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.878 kb on + strand, within Pf6N2E2_221at 279.879 kb on - strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.882 kb on + strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.883 kb on - strand, within Pf6N2E2_221at 279.945 kb on + strand, within Pf6N2E2_221at 279.945 kb on + strand, within Pf6N2E2_221at 279.946 kb on - strand, within Pf6N2E2_221at 279.946 kb on - strand, within Pf6N2E2_221at 280.004 kb on + strand, within Pf6N2E2_221at 280.004 kb on + strand, within Pf6N2E2_221at 280.004 kb on + strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.005 kb on - strand, within Pf6N2E2_221at 280.118 kb on + strand, within Pf6N2E2_221at 280.118 kb on + strand, within Pf6N2E2_221at 280.118 kb on + strand, within Pf6N2E2_221at 280.119 kb on - strand, within Pf6N2E2_221at 280.119 kb on - strand, within Pf6N2E2_221at 280.119 kb on - strand, within Pf6N2E2_221at 280.579 kb on + strandat 280.579 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.663 kb on + strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.664 kb on - strandat 280.904 kb on - strand, within Pf6N2E2_222at 280.904 kb on - strand, within Pf6N2E2_222at 280.904 kb on - strand, within Pf6N2E2_222at 281.258 kb on - strand, within Pf6N2E2_222at 281.258 kb on - strand, within Pf6N2E2_222at 281.383 kb on + strand, within Pf6N2E2_222at 281.383 kb on + strand, within Pf6N2E2_222at 281.481 kb on + strand, within Pf6N2E2_222at 281.482 kb on - strand, within Pf6N2E2_222at 281.614 kb on - strand, within Pf6N2E2_222at 281.839 kb on + strand, within Pf6N2E2_222at 282.016 kb on + strand, within Pf6N2E2_222at 282.016 kb on + strand, within Pf6N2E2_222at 282.016 kb on + strand, within Pf6N2E2_222at 282.016 kb on + strand, within Pf6N2E2_222at 282.016 kb on + strand, within Pf6N2E2_222at 282.016 kb on + strand, within Pf6N2E2_222at 282.017 kb on - strand, within Pf6N2E2_222at 282.017 kb on - strand, within Pf6N2E2_222at 282.018 kb on + strand, within Pf6N2E2_222at 282.019 kb on - strand, within Pf6N2E2_222at 282.019 kb on - strand, within Pf6N2E2_222at 282.071 kb on - strand, within Pf6N2E2_222at 282.071 kb on - strand, within Pf6N2E2_222at 282.170 kb on + strand, within Pf6N2E2_222at 282.388 kb on - strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.700 kb on + strand, within Pf6N2E2_222at 282.701 kb on - strand, within Pf6N2E2_222at 282.701 kb on - strand, within Pf6N2E2_222at 282.701 kb on - strand, within Pf6N2E2_222at 282.701 kb on - strand, within Pf6N2E2_222at 282.701 kb on - strand, within Pf6N2E2_222at 282.742 kb on + strand, within Pf6N2E2_222at 282.742 kb on + strand, within Pf6N2E2_222at 282.743 kb on - strand, within Pf6N2E2_222at 282.743 kb on - strand, within Pf6N2E2_222at 282.859 kb on + strand, within Pf6N2E2_222at 282.860 kb on - strand, within Pf6N2E2_222at 282.884 kb on - strand, within Pf6N2E2_222at 283.018 kb on + strand, within Pf6N2E2_222at 283.019 kb on - strand, within Pf6N2E2_222at 283.057 kb on + strand, within Pf6N2E2_222at 283.080 kb on - strand, within Pf6N2E2_222at 283.204 kb on + strand, within Pf6N2E2_222at 283.382 kb on + strandat 283.650 kb on + strandat 283.650 kb on + strandat 283.651 kb on - strandat 283.651 kb on - strandat 283.959 kb on + strand, within Pf6N2E2_223at 283.959 kb on + strand, within Pf6N2E2_223at 283.959 kb on + strand, within Pf6N2E2_223at 283.959 kb on + strand, within Pf6N2E2_223at 283.986 kb on + strand, within Pf6N2E2_223at 283.987 kb on - strand, within Pf6N2E2_223at 283.987 kb on - strand, within Pf6N2E2_223at 283.987 kb on - strand, within Pf6N2E2_223at 283.987 kb on - strand, within Pf6N2E2_223at 284.043 kb on + strand, within Pf6N2E2_223at 284.044 kb on - strand, within Pf6N2E2_223at 284.181 kb on + strand, within Pf6N2E2_223at 284.181 kb on + strand, within Pf6N2E2_223at 284.249 kb on + strand, within Pf6N2E2_223at 284.367 kb on + strand, within Pf6N2E2_223

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Per-strain Table

Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M
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279,444 + +0.2
279,522 - Pf6N2E2_221 0.24 +1.1
279,542 + Pf6N2E2_221 0.25 -0.8
279,542 + Pf6N2E2_221 0.25 -0.2
279,542 + Pf6N2E2_221 0.25 -1.7
279,542 + Pf6N2E2_221 0.25 +1.2
279,542 + Pf6N2E2_221 0.25 +1.1
279,543 - Pf6N2E2_221 0.25 +1.1
279,758 + Pf6N2E2_221 0.43 -0.4
279,758 + Pf6N2E2_221 0.43 -0.9
279,758 + Pf6N2E2_221 0.43 -0.2
279,759 - Pf6N2E2_221 0.44 +1.1
279,878 + Pf6N2E2_221 0.54 +0.1
279,878 + Pf6N2E2_221 0.54 +0.5
279,878 + Pf6N2E2_221 0.54 -0.5
279,878 + Pf6N2E2_221 0.54 -0.5
279,878 + Pf6N2E2_221 0.54 -0.3
279,878 + Pf6N2E2_221 0.54 +0.1
279,878 + Pf6N2E2_221 0.54 +0.6
279,879 - Pf6N2E2_221 0.54 -0.1
279,882 + Pf6N2E2_221 0.54 -0.4
279,882 + Pf6N2E2_221 0.54 +1.1
279,882 + Pf6N2E2_221 0.54 -0.4
279,882 + Pf6N2E2_221 0.54 -0.8
279,883 - Pf6N2E2_221 0.54 -0.9
279,883 - Pf6N2E2_221 0.54 -0.4
279,883 - Pf6N2E2_221 0.54 -1.1
279,883 - Pf6N2E2_221 0.54 -1.2
279,883 - Pf6N2E2_221 0.54 +0.8
279,883 - Pf6N2E2_221 0.54 +0.5
279,883 - Pf6N2E2_221 0.54 -0.8
279,945 + Pf6N2E2_221 0.59 -0.2
279,945 + Pf6N2E2_221 0.59 -0.5
279,946 - Pf6N2E2_221 0.59 +0.1
279,946 - Pf6N2E2_221 0.59 +1.1
280,004 + Pf6N2E2_221 0.64 +0.9
280,004 + Pf6N2E2_221 0.64 +0.6
280,004 + Pf6N2E2_221 0.64 +0.3
280,005 - Pf6N2E2_221 0.64 -1.2
280,005 - Pf6N2E2_221 0.64 -0.8
280,005 - Pf6N2E2_221 0.64 -0.4
280,005 - Pf6N2E2_221 0.64 -0.0
280,118 + Pf6N2E2_221 0.74 -1.1
280,118 + Pf6N2E2_221 0.74 -1.1
280,118 + Pf6N2E2_221 0.74 +0.5
280,119 - Pf6N2E2_221 0.74 -1.0
280,119 - Pf6N2E2_221 0.74 +0.2
280,119 - Pf6N2E2_221 0.74 -1.2
280,579 + +0.5
280,579 + +0.4
280,663 + -1.1
280,663 + -0.5
280,663 + -0.3
280,663 + -0.6
280,663 + -0.2
280,663 + +0.3
280,663 + -0.3
280,664 - -0.1
280,664 - -0.1
280,664 - +0.8
280,664 - -0.3
280,664 - +0.5
280,664 - -0.3
280,664 - +0.4
280,904 - Pf6N2E2_222 0.15 +1.2
280,904 - Pf6N2E2_222 0.15 -0.6
280,904 - Pf6N2E2_222 0.15 -2.0
281,258 - Pf6N2E2_222 0.26 +0.0
281,258 - Pf6N2E2_222 0.26 -1.3
281,383 + Pf6N2E2_222 0.30 -0.4
281,383 + Pf6N2E2_222 0.30 +0.0
281,481 + Pf6N2E2_222 0.33 -0.1
281,482 - Pf6N2E2_222 0.33 -0.8
281,614 - Pf6N2E2_222 0.37 +0.2
281,839 + Pf6N2E2_222 0.45 -0.8
282,016 + Pf6N2E2_222 0.50 +1.2
282,016 + Pf6N2E2_222 0.50 -0.5
282,016 + Pf6N2E2_222 0.50 -0.3
282,016 + Pf6N2E2_222 0.50 +0.5
282,016 + Pf6N2E2_222 0.50 +0.1
282,016 + Pf6N2E2_222 0.50 +1.0
282,017 - Pf6N2E2_222 0.50 -0.6
282,017 - Pf6N2E2_222 0.50 +0.8
282,018 + Pf6N2E2_222 0.50 +1.2
282,019 - Pf6N2E2_222 0.50 +0.8
282,019 - Pf6N2E2_222 0.50 -1.2
282,071 - Pf6N2E2_222 0.52 +1.0
282,071 - Pf6N2E2_222 0.52 -0.2
282,170 + Pf6N2E2_222 0.55 -0.1
282,388 - Pf6N2E2_222 0.62 -0.4
282,700 + Pf6N2E2_222 0.72 +0.4
282,700 + Pf6N2E2_222 0.72 -0.2
282,700 + Pf6N2E2_222 0.72 +0.6
282,700 + Pf6N2E2_222 0.72 -1.4
282,700 + Pf6N2E2_222 0.72 +0.0
282,700 + Pf6N2E2_222 0.72 -0.1
282,700 + Pf6N2E2_222 0.72 +1.5
282,700 + Pf6N2E2_222 0.72 -1.1
282,700 + Pf6N2E2_222 0.72 +0.5
282,700 + Pf6N2E2_222 0.72 -0.2
282,701 - Pf6N2E2_222 0.72 -0.8
282,701 - Pf6N2E2_222 0.72 -0.7
282,701 - Pf6N2E2_222 0.72 -2.1
282,701 - Pf6N2E2_222 0.72 +0.8
282,701 - Pf6N2E2_222 0.72 +0.2
282,742 + Pf6N2E2_222 0.73 +0.2
282,742 + Pf6N2E2_222 0.73 -0.0
282,743 - Pf6N2E2_222 0.73 -0.7
282,743 - Pf6N2E2_222 0.73 -1.2
282,859 + Pf6N2E2_222 0.77 -0.3
282,860 - Pf6N2E2_222 0.77 -0.5
282,884 - Pf6N2E2_222 0.78 -0.7
283,018 + Pf6N2E2_222 0.82 -0.2
283,019 - Pf6N2E2_222 0.82 -0.5
283,057 + Pf6N2E2_222 0.83 +0.2
283,080 - Pf6N2E2_222 0.84 -0.2
283,204 + Pf6N2E2_222 0.88 +0.9
283,382 + -1.1
283,650 + -0.2
283,650 + +0.2
283,651 - +1.4
283,651 - +0.3
283,959 + Pf6N2E2_223 0.27 +0.2
283,959 + Pf6N2E2_223 0.27 -0.9
283,959 + Pf6N2E2_223 0.27 +0.5
283,959 + Pf6N2E2_223 0.27 +0.6
283,986 + Pf6N2E2_223 0.29 -0.1
283,987 - Pf6N2E2_223 0.29 -1.0
283,987 - Pf6N2E2_223 0.29 +0.3
283,987 - Pf6N2E2_223 0.29 +0.5
283,987 - Pf6N2E2_223 0.29 -0.5
284,043 + Pf6N2E2_223 0.33 -0.4
284,044 - Pf6N2E2_223 0.33 +0.5
284,181 + Pf6N2E2_223 0.43 -0.4
284,181 + Pf6N2E2_223 0.43 -0.6
284,249 + Pf6N2E2_223 0.48 +0.7
284,367 + Pf6N2E2_223 0.56 -0.3

Or see this region's nucleotide sequence