Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2037 and Pf6N2E2_2038 are separated by 105 nucleotides Pf6N2E2_2038 and Pf6N2E2_2039 overlap by 1 nucleotides Pf6N2E2_2039 and Pf6N2E2_2040 are separated by 302 nucleotides
Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704
_2037
Pf6N2E2_2038 - Transcriptional regulator, TetR family, at 2,426,810 to 2,427,469
_2038
Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884
_2039
Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578
_2040
Position (kb)
2427
2428
2429 Strain fitness (log2 ratio)
-2
-1
0
1 at 2426.677 kb on + strand at 2426.869 kb on + strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.872 kb on - strand at 2426.872 kb on - strand at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.216 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.496 kb on + strand at 2427.496 kb on + strand at 2427.610 kb on + strand at 2427.610 kb on + strand at 2427.862 kb on + strand, within Pf6N2E2_2039 at 2427.925 kb on + strand, within Pf6N2E2_2039 at 2427.926 kb on - strand, within Pf6N2E2_2039 at 2428.228 kb on - strand, within Pf6N2E2_2039 at 2428.306 kb on + strand, within Pf6N2E2_2039 at 2428.417 kb on + strand, within Pf6N2E2_2039 at 2428.553 kb on + strand, within Pf6N2E2_2039 at 2428.554 kb on - strand, within Pf6N2E2_2039 at 2428.554 kb on - strand, within Pf6N2E2_2039 at 2428.677 kb on - strand, within Pf6N2E2_2039 at 2428.843 kb on + strand at 2428.844 kb on - strand at 2428.844 kb on - strand at 2428.882 kb on + strand at 2428.904 kb on + strand at 2428.906 kb on + strand at 2428.983 kb on - strand at 2429.167 kb on - strand at 2429.371 kb on - strand, within Pf6N2E2_2040 at 2429.468 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.517 kb on + strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.518 kb on - strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.588 kb on + strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.589 kb on - strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.591 kb on + strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040 at 2429.592 kb on - strand, within Pf6N2E2_2040
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 2,426,677 + -1.2 2,426,869 + -0.9 2,426,870 - -1.3 2,426,870 - +0.1 2,426,870 - +0.1 2,426,870 - +1.7 2,426,870 - -1.1 2,426,872 - -0.5 2,426,872 - -0.1 2,427,197 + Pf6N2E2_2038 0.59 +0.4 2,427,197 + Pf6N2E2_2038 0.59 +0.3 2,427,215 + Pf6N2E2_2038 0.61 -1.0 2,427,215 + Pf6N2E2_2038 0.61 -1.6 2,427,216 - Pf6N2E2_2038 0.62 +0.5 2,427,393 - Pf6N2E2_2038 0.88 +0.1 2,427,393 - Pf6N2E2_2038 0.88 -0.2 2,427,393 - Pf6N2E2_2038 0.88 +1.3 2,427,393 - Pf6N2E2_2038 0.88 -0.3 2,427,496 + -0.1 2,427,496 + -1.0 2,427,610 + +0.5 2,427,610 + +0.1 2,427,862 + Pf6N2E2_2039 0.28 -0.5 2,427,925 + Pf6N2E2_2039 0.32 -0.7 2,427,926 - Pf6N2E2_2039 0.32 -1.7 2,428,228 - Pf6N2E2_2039 0.54 -0.9 2,428,306 + Pf6N2E2_2039 0.59 +0.9 2,428,417 + Pf6N2E2_2039 0.67 -2.5 2,428,553 + Pf6N2E2_2039 0.77 -1.1 2,428,554 - Pf6N2E2_2039 0.77 -0.7 2,428,554 - Pf6N2E2_2039 0.77 -1.7 2,428,677 - Pf6N2E2_2039 0.85 +0.6 2,428,843 + -0.2 2,428,844 - -1.2 2,428,844 - -2.0 2,428,882 + -0.1 2,428,904 + +0.1 2,428,906 + -0.9 2,428,983 - -0.3 2,429,167 - -1.1 2,429,371 - Pf6N2E2_2040 0.13 -1.1 2,429,468 + Pf6N2E2_2040 0.20 +1.2 2,429,517 + Pf6N2E2_2040 0.24 +0.8 2,429,517 + Pf6N2E2_2040 0.24 +1.1 2,429,517 + Pf6N2E2_2040 0.24 -0.3 2,429,518 - Pf6N2E2_2040 0.24 -0.6 2,429,518 - Pf6N2E2_2040 0.24 +0.3 2,429,518 - Pf6N2E2_2040 0.24 +0.3 2,429,588 + Pf6N2E2_2040 0.29 -0.0 2,429,588 + Pf6N2E2_2040 0.29 +0.0 2,429,589 - Pf6N2E2_2040 0.29 +0.3 2,429,589 - Pf6N2E2_2040 0.29 +1.3 2,429,591 + Pf6N2E2_2040 0.29 +0.3 2,429,591 + Pf6N2E2_2040 0.29 -0.8 2,429,591 + Pf6N2E2_2040 0.29 +0.0 2,429,591 + Pf6N2E2_2040 0.29 -0.7 2,429,592 - Pf6N2E2_2040 0.29 +0.3 2,429,592 - Pf6N2E2_2040 0.29 +1.5
Or see this region's nucleotide sequence