Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1870 and Pf6N2E2_1871 are separated by 83 nucleotides Pf6N2E2_1871 and Pf6N2E2_1872 are separated by 3 nucleotides
Pf6N2E2_1870 - hypothetical protein, at 2,216,728 to 2,217,258
_1870
Pf6N2E2_1871 - Cytochrome c oxidase polypeptide III (EC 1.9.3.1), at 2,217,342 to 2,217,875
_1871
Pf6N2E2_1872 - hypothetical protein, at 2,217,879 to 2,218,154
_1872
Position (kb)
2217
2218 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2216.429 kb on + strand at 2216.430 kb on - strand at 2216.469 kb on + strand at 2216.470 kb on - strand at 2216.578 kb on + strand at 2216.578 kb on + strand at 2216.579 kb on - strand at 2216.579 kb on - strand at 2216.610 kb on - strand at 2216.683 kb on + strand at 2216.684 kb on - strand at 2216.684 kb on - strand at 2216.739 kb on + strand at 2216.740 kb on - strand at 2216.740 kb on - strand at 2216.740 kb on - strand at 2216.750 kb on - strand at 2216.750 kb on - strand at 2216.938 kb on + strand, within Pf6N2E2_1870 at 2216.946 kb on - strand, within Pf6N2E2_1870 at 2216.946 kb on - strand, within Pf6N2E2_1870 at 2217.263 kb on - strand at 2217.350 kb on + strand at 2217.497 kb on - strand, within Pf6N2E2_1871 at 2217.621 kb on + strand, within Pf6N2E2_1871 at 2217.621 kb on + strand, within Pf6N2E2_1871 at 2217.621 kb on + strand, within Pf6N2E2_1871 at 2217.622 kb on - strand, within Pf6N2E2_1871 at 2217.678 kb on + strand, within Pf6N2E2_1871 at 2217.679 kb on - strand, within Pf6N2E2_1871 at 2217.679 kb on - strand, within Pf6N2E2_1871 at 2217.739 kb on + strand, within Pf6N2E2_1871 at 2217.768 kb on + strand, within Pf6N2E2_1871 at 2217.768 kb on + strand, within Pf6N2E2_1871 at 2217.769 kb on - strand, within Pf6N2E2_1871 at 2217.814 kb on - strand, within Pf6N2E2_1871 at 2217.861 kb on + strand at 2217.861 kb on + strand at 2217.861 kb on + strand at 2217.862 kb on - strand at 2217.862 kb on - strand at 2217.873 kb on + strand at 2217.873 kb on + strand at 2217.874 kb on - strand at 2217.874 kb on - strand at 2217.876 kb on + strand at 2217.876 kb on + strand at 2217.876 kb on + strand at 2217.876 kb on + strand at 2217.876 kb on + strand at 2217.876 kb on + strand at 2217.877 kb on - strand at 2217.877 kb on - strand at 2217.877 kb on - strand at 2217.877 kb on - strand at 2217.877 kb on - strand at 2217.877 kb on - strand at 2217.877 kb on - strand at 2218.026 kb on - strand, within Pf6N2E2_1872 at 2218.128 kb on + strand at 2218.129 kb on - strand at 2218.365 kb on + strand at 2218.366 kb on - strand at 2218.366 kb on - strand at 2218.369 kb on + strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.370 kb on - strand at 2218.381 kb on + strand at 2218.478 kb on + strand at 2218.478 kb on + strand at 2218.478 kb on + strand at 2218.478 kb on + strand at 2218.479 kb on - strand at 2218.479 kb on - strand at 2218.479 kb on - strand at 2218.479 kb on - strand at 2218.479 kb on - strand at 2218.482 kb on + strand at 2218.482 kb on + strand at 2218.482 kb on + strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.483 kb on - strand at 2218.535 kb on + strand at 2218.535 kb on + strand at 2218.535 kb on + strand at 2218.535 kb on + strand at 2218.536 kb on - strand at 2218.536 kb on - strand at 2218.584 kb on + strand at 2218.675 kb on + strand at 2218.675 kb on + strand at 2218.685 kb on + strand at 2218.686 kb on - strand at 2218.702 kb on + strand at 2218.702 kb on + strand at 2218.702 kb on + strand at 2218.857 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 2,216,429 + +0.1 2,216,430 - -0.1 2,216,469 + -0.8 2,216,470 - +1.7 2,216,578 + +0.3 2,216,578 + -0.2 2,216,579 - +1.1 2,216,579 - -0.1 2,216,610 - +0.5 2,216,683 + +1.1 2,216,684 - +0.0 2,216,684 - +0.2 2,216,739 + +0.1 2,216,740 - +0.7 2,216,740 - +0.5 2,216,740 - -0.5 2,216,750 - -0.4 2,216,750 - +0.8 2,216,938 + Pf6N2E2_1870 0.40 -1.0 2,216,946 - Pf6N2E2_1870 0.41 -0.6 2,216,946 - Pf6N2E2_1870 0.41 +0.5 2,217,263 - +0.8 2,217,350 + +2.3 2,217,497 - Pf6N2E2_1871 0.29 +1.4 2,217,621 + Pf6N2E2_1871 0.52 -0.5 2,217,621 + Pf6N2E2_1871 0.52 -1.4 2,217,621 + Pf6N2E2_1871 0.52 -0.5 2,217,622 - Pf6N2E2_1871 0.52 -0.5 2,217,678 + Pf6N2E2_1871 0.63 -1.6 2,217,679 - Pf6N2E2_1871 0.63 -0.1 2,217,679 - Pf6N2E2_1871 0.63 -0.5 2,217,739 + Pf6N2E2_1871 0.74 -2.8 2,217,768 + Pf6N2E2_1871 0.80 -1.1 2,217,768 + Pf6N2E2_1871 0.80 +0.4 2,217,769 - Pf6N2E2_1871 0.80 -0.3 2,217,814 - Pf6N2E2_1871 0.88 +1.1 2,217,861 + -0.5 2,217,861 + -0.2 2,217,861 + +0.9 2,217,862 - +0.7 2,217,862 - -0.7 2,217,873 + -0.5 2,217,873 + +0.4 2,217,874 - -0.6 2,217,874 - -0.1 2,217,876 + -0.1 2,217,876 + +0.2 2,217,876 + +0.3 2,217,876 + -0.6 2,217,876 + -0.3 2,217,876 + +0.3 2,217,877 - +0.1 2,217,877 - -1.0 2,217,877 - +0.7 2,217,877 - -0.8 2,217,877 - -0.6 2,217,877 - -1.0 2,217,877 - -1.3 2,218,026 - Pf6N2E2_1872 0.53 +0.4 2,218,128 + +0.1 2,218,129 - -0.9 2,218,365 + +0.2 2,218,366 - +0.4 2,218,366 - +0.8 2,218,369 + +1.4 2,218,370 - +1.3 2,218,370 - +0.2 2,218,370 - +0.1 2,218,370 - +0.5 2,218,370 - -0.5 2,218,370 - -0.2 2,218,370 - +0.5 2,218,370 - +0.7 2,218,381 + +0.4 2,218,478 + +1.7 2,218,478 + -0.5 2,218,478 + +0.6 2,218,478 + -0.2 2,218,479 - +0.5 2,218,479 - -0.1 2,218,479 - -0.5 2,218,479 - +0.8 2,218,479 - -0.6 2,218,482 + +1.5 2,218,482 + +0.5 2,218,482 + +0.2 2,218,483 - +0.7 2,218,483 - +0.2 2,218,483 - +0.0 2,218,483 - -0.5 2,218,483 - +1.1 2,218,483 - -1.9 2,218,483 - -0.3 2,218,483 - -0.1 2,218,535 + +1.0 2,218,535 + +0.1 2,218,535 + +0.2 2,218,535 + +1.1 2,218,536 - -2.5 2,218,536 - +1.1 2,218,584 + -0.4 2,218,675 + -1.1 2,218,675 + +0.6 2,218,685 + +0.1 2,218,686 - -0.4 2,218,702 + +0.7 2,218,702 + +0.3 2,218,702 + -0.2 2,218,857 - +0.7
Or see this region's nucleotide sequence