Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1667 and Pf6N2E2_1668 are separated by 43 nucleotides Pf6N2E2_1668 and Pf6N2E2_1669 are separated by 134 nucleotides Pf6N2E2_1669 and Pf6N2E2_1670 are separated by 62 nucleotides Pf6N2E2_1670 and Pf6N2E2_1671 are separated by 24 nucleotides
Pf6N2E2_1667 - Fumarylacetoacetate hydrolase family protein, at 1,973,376 to 1,974,557
_1667
Pf6N2E2_1668 - Xylonate dehydratase (EC 4.2.1.82) (from data) , at 1,974,601 to 1,976,385
_1668
Pf6N2E2_1669 - Probable tautomerase, at 1,976,520 to 1,976,738
_1669
Pf6N2E2_1670 - conserved membrane protein, at 1,976,801 to 1,977,310
_1670
Pf6N2E2_1671 - Transcriptional regulator, TetR family, at 1,977,335 to 1,978,012
_1671
Position (kb)
1974
1975
1976
1977 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1973.865 kb on - strand, within Pf6N2E2_1667 at 1974.224 kb on - strand, within Pf6N2E2_1667 at 1974.525 kb on - strand at 1974.582 kb on + strand at 1974.583 kb on - strand at 1974.583 kb on - strand at 1974.679 kb on + strand at 1974.691 kb on + strand at 1974.700 kb on + strand at 1974.701 kb on - strand at 1974.701 kb on - strand at 1974.919 kb on + strand, within Pf6N2E2_1668 at 1974.920 kb on - strand, within Pf6N2E2_1668 at 1974.920 kb on - strand, within Pf6N2E2_1668 at 1974.949 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.106 kb on + strand, within Pf6N2E2_1668 at 1975.206 kb on + strand, within Pf6N2E2_1668 at 1975.207 kb on - strand, within Pf6N2E2_1668 at 1975.315 kb on + strand, within Pf6N2E2_1668 at 1975.315 kb on + strand, within Pf6N2E2_1668 at 1975.315 kb on + strand, within Pf6N2E2_1668 at 1975.316 kb on - strand, within Pf6N2E2_1668 at 1975.556 kb on - strand, within Pf6N2E2_1668 at 1975.556 kb on - strand, within Pf6N2E2_1668 at 1975.825 kb on + strand, within Pf6N2E2_1668 at 1975.825 kb on + strand, within Pf6N2E2_1668 at 1975.825 kb on + strand, within Pf6N2E2_1668 at 1975.826 kb on - strand, within Pf6N2E2_1668 at 1975.826 kb on - strand, within Pf6N2E2_1668 at 1975.826 kb on - strand, within Pf6N2E2_1668 at 1975.851 kb on + strand, within Pf6N2E2_1668 at 1975.852 kb on - strand, within Pf6N2E2_1668 at 1975.852 kb on - strand, within Pf6N2E2_1668 at 1975.874 kb on + strand, within Pf6N2E2_1668 at 1975.874 kb on + strand, within Pf6N2E2_1668 at 1975.894 kb on + strand, within Pf6N2E2_1668 at 1975.895 kb on - strand, within Pf6N2E2_1668 at 1976.247 kb on + strand at 1976.282 kb on - strand at 1976.441 kb on + strand at 1976.441 kb on + strand at 1976.441 kb on + strand at 1976.442 kb on - strand at 1976.501 kb on + strand at 1976.535 kb on + strand at 1976.536 kb on - strand at 1976.536 kb on - strand at 1976.536 kb on - strand at 1976.613 kb on + strand, within Pf6N2E2_1669 at 1976.744 kb on - strand at 1976.750 kb on + strand at 1976.922 kb on - strand, within Pf6N2E2_1670 at 1976.922 kb on - strand, within Pf6N2E2_1670 at 1977.182 kb on + strand, within Pf6N2E2_1670 at 1977.183 kb on - strand, within Pf6N2E2_1670 at 1977.211 kb on - strand, within Pf6N2E2_1670 at 1977.258 kb on - strand, within Pf6N2E2_1670 at 1977.284 kb on + strand at 1977.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 1,973,865 - Pf6N2E2_1667 0.41 +0.2 1,974,224 - Pf6N2E2_1667 0.72 -0.4 1,974,525 - -1.0 1,974,582 + -0.0 1,974,583 - +0.6 1,974,583 - -0.3 1,974,679 + +0.6 1,974,691 + +0.2 1,974,700 + +2.0 1,974,701 - -0.4 1,974,701 - +1.0 1,974,919 + Pf6N2E2_1668 0.18 -0.3 1,974,920 - Pf6N2E2_1668 0.18 +1.2 1,974,920 - Pf6N2E2_1668 0.18 -0.3 1,974,949 + Pf6N2E2_1668 0.19 -0.7 1,975,106 + Pf6N2E2_1668 0.28 -0.3 1,975,106 + Pf6N2E2_1668 0.28 -0.4 1,975,106 + Pf6N2E2_1668 0.28 -0.2 1,975,106 + Pf6N2E2_1668 0.28 -1.3 1,975,206 + Pf6N2E2_1668 0.34 -0.7 1,975,207 - Pf6N2E2_1668 0.34 -0.3 1,975,315 + Pf6N2E2_1668 0.40 -0.6 1,975,315 + Pf6N2E2_1668 0.40 -0.7 1,975,315 + Pf6N2E2_1668 0.40 -0.2 1,975,316 - Pf6N2E2_1668 0.40 -0.7 1,975,556 - Pf6N2E2_1668 0.54 -1.5 1,975,556 - Pf6N2E2_1668 0.54 +1.8 1,975,825 + Pf6N2E2_1668 0.69 -1.6 1,975,825 + Pf6N2E2_1668 0.69 +0.8 1,975,825 + Pf6N2E2_1668 0.69 -1.4 1,975,826 - Pf6N2E2_1668 0.69 +1.0 1,975,826 - Pf6N2E2_1668 0.69 +0.6 1,975,826 - Pf6N2E2_1668 0.69 -0.4 1,975,851 + Pf6N2E2_1668 0.70 +0.5 1,975,852 - Pf6N2E2_1668 0.70 +0.3 1,975,852 - Pf6N2E2_1668 0.70 -0.4 1,975,874 + Pf6N2E2_1668 0.71 -0.3 1,975,874 + Pf6N2E2_1668 0.71 +0.4 1,975,894 + Pf6N2E2_1668 0.72 +1.0 1,975,895 - Pf6N2E2_1668 0.72 -2.1 1,976,247 + +0.8 1,976,282 - +0.2 1,976,441 + +0.8 1,976,441 + -0.3 1,976,441 + -0.7 1,976,442 - -1.2 1,976,501 + +0.3 1,976,535 + -1.1 1,976,536 - +0.3 1,976,536 - +0.1 1,976,536 - -1.0 1,976,613 + Pf6N2E2_1669 0.42 -0.8 1,976,744 - +1.0 1,976,750 + +1.2 1,976,922 - Pf6N2E2_1670 0.24 -0.6 1,976,922 - Pf6N2E2_1670 0.24 +0.4 1,977,182 + Pf6N2E2_1670 0.75 +0.3 1,977,183 - Pf6N2E2_1670 0.75 +0.1 1,977,211 - Pf6N2E2_1670 0.80 +0.5 1,977,258 - Pf6N2E2_1670 0.90 +0.9 1,977,284 + +1.1 1,977,284 + +0.0
Or see this region's nucleotide sequence