Experiment: Magnesium chloride 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_130 and Pf6N2E2_131 are separated by 31 nucleotides Pf6N2E2_131 and Pf6N2E2_132 are separated by 219 nucleotides Pf6N2E2_132 and Pf6N2E2_133 are separated by 42 nucleotides Pf6N2E2_133 and Pf6N2E2_134 are separated by 342 nucleotides
Pf6N2E2_130 - hypothetical protein, at 180,710 to 180,952
_130
Pf6N2E2_131 - hypothetical protein, at 180,984 to 181,157
_131
Pf6N2E2_132 - hcp, at 181,377 to 181,658
_132
Pf6N2E2_133 - hypothetical protein, at 181,701 to 181,850
_133
Pf6N2E2_134 - FIG00960493: hypothetical protein, at 182,193 to 183,569
_134
Position (kb) 181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 180.773 kb on + strand, within Pf6N2E2_130 at 181.179 kb on - strand at 181.186 kb on + strand at 181.205 kb on - strand at 181.221 kb on + strand at 181.221 kb on + strand at 181.221 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.224 kb on + strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.225 kb on - strand at 181.255 kb on + strand at 181.256 kb on - strand at 181.312 kb on - strand at 181.321 kb on + strand at 181.321 kb on + strand at 181.428 kb on + strand, within Pf6N2E2_132 at 181.428 kb on + strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.429 kb on - strand, within Pf6N2E2_132 at 181.519 kb on + strand, within Pf6N2E2_132 at 181.519 kb on + strand, within Pf6N2E2_132 at 181.564 kb on + strand, within Pf6N2E2_132 at 181.565 kb on - strand, within Pf6N2E2_132 at 181.565 kb on - strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.567 kb on + strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.568 kb on - strand, within Pf6N2E2_132 at 181.683 kb on - strand at 181.692 kb on + strand at 181.693 kb on - strand at 181.693 kb on - strand at 181.705 kb on - strand at 181.705 kb on - strand at 181.790 kb on + strand, within Pf6N2E2_133 at 181.811 kb on + strand, within Pf6N2E2_133 at 181.829 kb on + strand, within Pf6N2E2_133 at 181.851 kb on + strand at 181.851 kb on + strand at 181.851 kb on + strand at 181.851 kb on + strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.852 kb on - strand at 181.879 kb on + strand at 181.879 kb on + strand at 181.879 kb on + strand at 181.880 kb on - strand at 182.188 kb on + strand at 182.320 kb on + strand at 182.320 kb on + strand at 182.392 kb on + strand, within Pf6N2E2_134 at 182.392 kb on + strand, within Pf6N2E2_134 at 182.393 kb on - strand, within Pf6N2E2_134 at 182.393 kb on - strand, within Pf6N2E2_134 at 182.416 kb on + strand, within Pf6N2E2_134 at 182.416 kb on + strand, within Pf6N2E2_134 at 182.417 kb on - strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.506 kb on + strand, within Pf6N2E2_134 at 182.581 kb on + strand, within Pf6N2E2_134 at 182.582 kb on - strand, within Pf6N2E2_134 at 182.582 kb on - strand, within Pf6N2E2_134
Per-strain Table
Position Strand Gene LocusTag Fraction Magnesium chloride 0.125 M remove 180,773 + Pf6N2E2_130 0.26 -0.2 181,179 - +0.4 181,186 + +0.8 181,205 - +0.0 181,221 + -0.7 181,221 + +0.0 181,221 + -1.2 181,224 + +0.6 181,224 + -0.0 181,224 + -0.6 181,224 + -0.2 181,224 + +0.4 181,224 + -0.9 181,224 + -0.0 181,224 + -1.1 181,225 - +0.8 181,225 - +1.2 181,225 - -2.1 181,225 - +0.2 181,255 + +1.8 181,256 - +1.8 181,312 - +1.2 181,321 + -0.8 181,321 + -0.5 181,428 + Pf6N2E2_132 0.18 -1.6 181,428 + Pf6N2E2_132 0.18 -0.2 181,429 - Pf6N2E2_132 0.18 +0.2 181,429 - Pf6N2E2_132 0.18 -0.7 181,429 - Pf6N2E2_132 0.18 +0.3 181,519 + Pf6N2E2_132 0.50 +1.4 181,519 + Pf6N2E2_132 0.50 +0.8 181,564 + Pf6N2E2_132 0.66 +0.1 181,565 - Pf6N2E2_132 0.67 -1.0 181,565 - Pf6N2E2_132 0.67 +0.3 181,567 + Pf6N2E2_132 0.67 +1.1 181,567 + Pf6N2E2_132 0.67 -1.9 181,567 + Pf6N2E2_132 0.67 +2.5 181,568 - Pf6N2E2_132 0.68 +0.2 181,568 - Pf6N2E2_132 0.68 +0.0 181,568 - Pf6N2E2_132 0.68 -2.8 181,683 - -0.7 181,692 + +0.8 181,693 - +2.4 181,693 - -0.1 181,705 - -0.5 181,705 - -0.1 181,790 + Pf6N2E2_133 0.59 -0.7 181,811 + Pf6N2E2_133 0.73 -0.3 181,829 + Pf6N2E2_133 0.85 -0.9 181,851 + +0.1 181,851 + -0.5 181,851 + +0.5 181,851 + +0.4 181,852 - -0.3 181,852 - -0.6 181,852 - +0.8 181,852 - +0.1 181,852 - +1.3 181,879 + +0.4 181,879 + -0.5 181,879 + -1.5 181,880 - -1.0 182,188 + +0.0 182,320 + -0.2 182,320 + -1.1 182,392 + Pf6N2E2_134 0.14 +0.4 182,392 + Pf6N2E2_134 0.14 -0.3 182,393 - Pf6N2E2_134 0.15 +0.4 182,393 - Pf6N2E2_134 0.15 +0.8 182,416 + Pf6N2E2_134 0.16 +0.0 182,416 + Pf6N2E2_134 0.16 +0.2 182,417 - Pf6N2E2_134 0.16 -0.7 182,506 + Pf6N2E2_134 0.23 +1.0 182,506 + Pf6N2E2_134 0.23 +0.3 182,506 + Pf6N2E2_134 0.23 -0.7 182,506 + Pf6N2E2_134 0.23 -0.0 182,581 + Pf6N2E2_134 0.28 +0.1 182,582 - Pf6N2E2_134 0.28 -0.9 182,582 - Pf6N2E2_134 0.28 +0.2
Or see this region's nucleotide sequence