Experiment: LB_Aerobic_18HR_ZN18A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_15520 and CSW01_15525 are separated by 153 nucleotides CSW01_15525 and CSW01_15530 are separated by 0 nucleotides CSW01_15530 and CSW01_15535 are separated by 243 nucleotides CSW01_15535 and CSW01_15540 are separated by 56 nucleotides CSW01_15540 and CSW01_15545 overlap by 52 nucleotides CSW01_15545 and CSW01_15550 overlap by 25 nucleotides CSW01_15550 and CSW01_15555 are separated by 123 nucleotides
CSW01_15520: CSW01_15520 - hypothetical protein, at 320,347 to 320,712
_15520
CSW01_15525: CSW01_15525 - type II toxin-antitoxin system RelE/ParE family toxin, at 320,866 to 321,201
_15525
CSW01_15530: CSW01_15530 - type II toxin-antitoxin system Phd/YefM family antitoxin, at 321,202 to 321,444
_15530
CSW01_15535: CSW01_15535 - NUDIX hydrolase, at 321,688 to 322,074
_15535
CSW01_15540: CSW01_15540 - acetyltransferase, at 322,131 to 322,244
_15540
CSW01_15545: CSW01_15545 - DUF1289 domain-containing protein, at 322,193 to 322,474
_15545
CSW01_15550: CSW01_15550 - DUF1196 domain-containing protein, at 322,450 to 322,608
_15550
CSW01_15555: CSW01_15555 - N-acetyltransferase, at 322,732 to 323,268
_15555
Position (kb) 321
322
323 Strain fitness (log2 ratio) -1
0
1 at 320.705 kb on + strand at 320.713 kb on - strand at 320.723 kb on - strand at 320.723 kb on - strand at 321.661 kb on + strand at 321.669 kb on - strand at 321.788 kb on + strand, within CSW01_15535 at 321.788 kb on + strand, within CSW01_15535 at 321.930 kb on - strand, within CSW01_15535 at 321.949 kb on - strand, within CSW01_15535 at 321.989 kb on + strand, within CSW01_15535
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Aerobic_18HR_ZN18A remove 320,705 + -0.5 320,713 - +0.2 320,723 - -1.4 320,723 - -0.4 321,661 + -0.3 321,669 - -0.3 321,788 + CSW01_15535 0.26 -0.2 321,788 + CSW01_15535 0.26 -1.0 321,930 - CSW01_15535 0.63 -1.6 321,949 - CSW01_15535 0.67 -0.3 321,989 + CSW01_15535 0.78 +0.2
Or see this region's nucleotide sequence