Experiment: Recovery broth
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13695 and BBR_RS13700 are separated by 130 nucleotides BBR_RS13700 and BBR_RS13705 are separated by 68 nucleotides BBR_RS13705 and BBR_RS13710 are separated by 126 nucleotides
BBR_RS13695: BBR_RS13695 - glutamate racemase, at 846,186 to 846,980
_RS13695
BBR_RS13700: BBR_RS13700 - diaminopimelate epimerase, at 847,111 to 848,010
_RS13700
BBR_RS13705: BBR_RS13705 - Conserved hypothetical membrane spanning protein, contains a vitamin K epoxide reductase domain, at 848,079 to 848,798
_RS13705
BBR_RS13710: BBR_RS13710 - PHP domain-containing protein, at 848,925 to 849,818
_RS13710
Position (kb)
847
848
849 Strain fitness (log2 ratio)
-1
0
1
2 at 846.117 kb on + strand at 846.173 kb on - strand at 846.208 kb on - strand at 846.212 kb on + strand at 846.220 kb on - strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.043 kb on + strand at 847.051 kb on - strand at 847.058 kb on + strand at 847.077 kb on + strand at 847.098 kb on - strand at 847.098 kb on - strand at 847.169 kb on + strand at 847.216 kb on - strand, within BBR_RS13700 at 847.252 kb on + strand, within BBR_RS13700 at 847.266 kb on + strand, within BBR_RS13700 at 847.266 kb on + strand, within BBR_RS13700 at 847.269 kb on - strand, within BBR_RS13700 at 847.283 kb on + strand, within BBR_RS13700 at 847.288 kb on + strand, within BBR_RS13700 at 847.338 kb on - strand, within BBR_RS13700 at 847.360 kb on + strand, within BBR_RS13700 at 847.412 kb on - strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.419 kb on + strand, within BBR_RS13700 at 847.447 kb on - strand, within BBR_RS13700 at 847.480 kb on + strand, within BBR_RS13700 at 847.489 kb on + strand, within BBR_RS13700 at 847.500 kb on + strand, within BBR_RS13700 at 847.507 kb on - strand, within BBR_RS13700 at 847.514 kb on + strand, within BBR_RS13700 at 847.514 kb on + strand, within BBR_RS13700 at 847.522 kb on - strand, within BBR_RS13700 at 847.522 kb on - strand, within BBR_RS13700 at 847.568 kb on + strand, within BBR_RS13700 at 847.568 kb on + strand, within BBR_RS13700 at 847.568 kb on + strand, within BBR_RS13700 at 847.576 kb on - strand, within BBR_RS13700 at 847.576 kb on - strand, within BBR_RS13700 at 847.639 kb on - strand, within BBR_RS13700 at 847.688 kb on - strand, within BBR_RS13700 at 847.713 kb on + strand, within BBR_RS13700 at 847.726 kb on - strand, within BBR_RS13700 at 847.771 kb on + strand, within BBR_RS13700 at 847.783 kb on + strand, within BBR_RS13700 at 847.826 kb on + strand, within BBR_RS13700 at 847.834 kb on - strand, within BBR_RS13700 at 847.852 kb on - strand, within BBR_RS13700 at 847.854 kb on + strand, within BBR_RS13700 at 847.870 kb on + strand, within BBR_RS13700 at 847.878 kb on + strand, within BBR_RS13700 at 847.900 kb on - strand, within BBR_RS13700 at 847.925 kb on + strand at 847.952 kb on + strand at 847.953 kb on + strand at 847.974 kb on - strand at 847.993 kb on + strand at 848.003 kb on + strand at 848.006 kb on - strand at 848.020 kb on + strand at 848.101 kb on - strand at 848.103 kb on - strand at 848.145 kb on - strand at 848.291 kb on - strand, within BBR_RS13705 at 848.291 kb on - strand, within BBR_RS13705 at 848.291 kb on - strand, within BBR_RS13705 at 848.305 kb on + strand, within BBR_RS13705 at 848.305 kb on + strand, within BBR_RS13705 at 848.321 kb on - strand, within BBR_RS13705 at 848.350 kb on + strand, within BBR_RS13705 at 848.368 kb on - strand, within BBR_RS13705 at 848.387 kb on - strand, within BBR_RS13705 at 848.388 kb on + strand, within BBR_RS13705 at 848.391 kb on - strand, within BBR_RS13705 at 848.396 kb on - strand, within BBR_RS13705 at 848.481 kb on - strand, within BBR_RS13705 at 848.505 kb on - strand, within BBR_RS13705 at 848.610 kb on + strand, within BBR_RS13705 at 848.618 kb on - strand, within BBR_RS13705 at 848.694 kb on + strand, within BBR_RS13705 at 848.707 kb on - strand, within BBR_RS13705 at 848.721 kb on + strand, within BBR_RS13705 at 848.728 kb on - strand at 848.819 kb on - strand at 848.927 kb on + strand at 848.938 kb on - strand at 848.940 kb on + strand at 848.940 kb on - strand at 848.940 kb on - strand at 848.953 kb on + strand at 848.953 kb on + strand at 848.963 kb on + strand at 848.971 kb on - strand at 848.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth remove 846,117 + +0.5 846,173 - -0.1 846,208 - -0.5 846,212 + +1.4 846,220 - +0.5 847,043 + -0.5 847,043 + +0.5 847,043 + -0.0 847,043 + -0.1 847,043 + +0.2 847,043 + +1.8 847,043 + -0.2 847,051 - +0.6 847,058 + +0.3 847,077 + +0.7 847,098 - -0.4 847,098 - +0.4 847,169 + -0.1 847,216 - BBR_RS13700 0.12 -0.1 847,252 + BBR_RS13700 0.16 +0.2 847,266 + BBR_RS13700 0.17 +0.1 847,266 + BBR_RS13700 0.17 -0.9 847,269 - BBR_RS13700 0.18 +0.2 847,283 + BBR_RS13700 0.19 +0.8 847,288 + BBR_RS13700 0.20 -0.3 847,338 - BBR_RS13700 0.25 +0.7 847,360 + BBR_RS13700 0.28 +0.5 847,412 - BBR_RS13700 0.33 +0.2 847,419 + BBR_RS13700 0.34 -1.6 847,419 + BBR_RS13700 0.34 +0.3 847,419 + BBR_RS13700 0.34 -0.7 847,419 + BBR_RS13700 0.34 +0.7 847,447 - BBR_RS13700 0.37 +1.2 847,480 + BBR_RS13700 0.41 +0.6 847,489 + BBR_RS13700 0.42 +0.8 847,500 + BBR_RS13700 0.43 -0.0 847,507 - BBR_RS13700 0.44 -0.1 847,514 + BBR_RS13700 0.45 -0.1 847,514 + BBR_RS13700 0.45 +0.0 847,522 - BBR_RS13700 0.46 -0.8 847,522 - BBR_RS13700 0.46 +0.5 847,568 + BBR_RS13700 0.51 +1.5 847,568 + BBR_RS13700 0.51 +2.1 847,568 + BBR_RS13700 0.51 +0.5 847,576 - BBR_RS13700 0.52 +0.5 847,576 - BBR_RS13700 0.52 +0.4 847,639 - BBR_RS13700 0.59 +0.2 847,688 - BBR_RS13700 0.64 +1.2 847,713 + BBR_RS13700 0.67 +0.2 847,726 - BBR_RS13700 0.68 +0.2 847,771 + BBR_RS13700 0.73 -0.4 847,783 + BBR_RS13700 0.75 +0.3 847,826 + BBR_RS13700 0.79 +1.0 847,834 - BBR_RS13700 0.80 -0.8 847,852 - BBR_RS13700 0.82 +0.9 847,854 + BBR_RS13700 0.83 +1.3 847,870 + BBR_RS13700 0.84 +0.1 847,878 + BBR_RS13700 0.85 -0.8 847,900 - BBR_RS13700 0.88 +0.8 847,925 + -0.2 847,952 + -0.4 847,953 + -0.3 847,974 - +0.1 847,993 + -0.1 848,003 + +0.2 848,006 - +0.2 848,020 + -1.3 848,101 - +0.2 848,103 - -0.1 848,145 - +1.1 848,291 - BBR_RS13705 0.29 +0.2 848,291 - BBR_RS13705 0.29 +0.4 848,291 - BBR_RS13705 0.29 -0.8 848,305 + BBR_RS13705 0.31 -0.3 848,305 + BBR_RS13705 0.31 -1.2 848,321 - BBR_RS13705 0.34 +1.0 848,350 + BBR_RS13705 0.38 -0.6 848,368 - BBR_RS13705 0.40 -0.7 848,387 - BBR_RS13705 0.43 -0.3 848,388 + BBR_RS13705 0.43 -1.1 848,391 - BBR_RS13705 0.43 -0.1 848,396 - BBR_RS13705 0.44 -0.3 848,481 - BBR_RS13705 0.56 -0.9 848,505 - BBR_RS13705 0.59 -0.8 848,610 + BBR_RS13705 0.74 +0.5 848,618 - BBR_RS13705 0.75 -0.2 848,694 + BBR_RS13705 0.85 -1.1 848,707 - BBR_RS13705 0.87 +0.4 848,721 + BBR_RS13705 0.89 +1.2 848,728 - -0.6 848,819 - -0.1 848,927 + +0.0 848,938 - +0.2 848,940 + +1.6 848,940 - +0.2 848,940 - +1.2 848,953 + +0.3 848,953 + +0.2 848,963 + +0.9 848,971 - -0.2 848,971 - -0.3
Or see this region's nucleotide sequence